Patterns of Autologous and Nonautologous Interactions between Core Nuclear Egress Complex (NEC) Proteins of α-, β- and γ-Herpesviruses [PDF]
Viruses, 2020 Nuclear egress is a regulated process shared by α-, β- and γ-herpesviruses. The core nuclear egress complex (NEC) is composed of the membrane-anchored protein homologs of human cytomegalovirus (HCMV) pUL50, murine cytomegalovirus (MCMV ...
Sigrun Häge +11 more
doaj +2 more sources
Expression, Purification and Crystallization of the Herpesvirus Nuclear Egress Complex (NEC) [PDF]
Bio-Protocol, 2016 The protocol describes the production and crystallization of the soluble form of the nuclear egress complex (NEC) from Herpes simplex virus 1 and Pseudorabies virus. The NEC is a heterodimer that consists of conserved proteins UL31 and UL34.
Janna Bigalke, Ekaterina Heldwein
doaj +3 more sources
Nuclear entry and egress of parvoviruses
Molecular Microbiology, 2022 AbstractParvoviruses are small non‐enveloped single‐stranded DNA viruses, which depend on host cell nuclear transcriptional and replication machinery. After endosomal exposure of nuclear localization sequence and a phospholipase A2 domain on the capsid surface, and escape into the cytosol, parvovirus capsids enter the nucleus.
Mattola, Salla +5 more
+7 more sources
Molecular plasticity of herpesvirus nuclear egress analysed in situ. [PDF]
Nat MicrobiolThe viral nuclear egress complex (NEC) allows herpesvirus capsids to escape from the nucleus without compromising the nuclear envelope integrity. The NEC lattice assembles on the inner nuclear membrane and mediates the budding of nascent nucleocapsids into the perinuclear space and their subsequent release into the cytosol.
Pražák V +12 more
europepmc +4 more sources
A beta-herpesvirus with fluorescent capsids to study transport in living cells. [PDF]
, 2012 Fluorescent tagging of viral particles by genetic means enables the study of virus dynamics in living cells. However, the study of beta-herpesvirus entry and morphogenesis by this method is currently limited.
Bauerfeind, Rudolf +11 more
core +9 more sources
The Herpes Simplex Virus Protein pUL31 Escorts Nucleocapsids to Sites of Nuclear Egress, a Process Coordinated by Its N-Terminal Domain. [PDF]
PLoS Pathogens, 2015 Progeny capsids of herpesviruses leave the nucleus by budding through the nuclear envelope. Two viral proteins, the membrane protein pUL34 and the nucleo-phosphoprotein pUL31 form the nuclear egress complex that is required for capsid egress out of the ...
Christina Funk +7 more
doaj +1 more source
Differentiating the Roles of UL16, UL21, and Us3 in the Nuclear Egress of Herpes Simplex Virus Capsids. [PDF]
J Virol, 2020 Gao J +4 more
europepmc +2 more sources
Baculovirus Actin-Based Motility Drives Nuclear Envelope Disruption and Nuclear Egress [PDF]
Current Biology, 2018 Viruses that replicate in the host cell nucleus face challenges in usurping cellular pathways to enable passage through the nuclear envelope [1]. Baculoviruses are enveloped, double-stranded DNA viruses that infect lepidopteran insects and are tools for protein expression, cell transduction, and pest management [2-4].
Ohkawa, Taro, Welch, Matthew D
openaire +4 more sources
Nuclear envelope budding and its cellular functions
Nucleus, 2023 The nuclear pore complex (NPC) has long been assumed to be the sole route across the nuclear envelope, and under normal homeostatic conditions it is indeed the main mechanism of nucleo-cytoplasmic transport.
Katharina S. Keuenhof +6 more
doaj +1 more source
Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress [PDF]
Journal of Virology, 2016 ABSTRACT Human cytomegalovirus (HCMV) pUL93 and pUL77 are both essential for virus growth, but their functions in the virus life cycle remain mostly unresolved. Homologs of pUL93 and pUL77 in herpes simplex virus 1 (HSV-1) and pseudorabies virus (PRV) are known to interact to form a complex at capsid vertices known as the capsid vertex ...
Bernadette M, DeRussy +2 more
openaire +2 more sources