Results 11 to 20 of about 26,611 (238)

Expression, Purification and Crystallization of the Herpesvirus Nuclear Egress Complex (NEC) [PDF]

Bio-Protocol, 2016
The protocol describes the production and crystallization of the soluble form of the nuclear egress complex (NEC) from Herpes simplex virus 1 and Pseudorabies virus. The NEC is a heterodimer that consists of conserved proteins UL31 and UL34.
Janna Bigalke, Ekaterina Heldwein
doaj   +5 more sources

Virus-derived peptide inhibitors of the herpes simplex virus type 1 nuclear egress complex [PDF]

Scientific Reports, 2021
Herpesviruses infect a majority of the human population, establishing lifelong latent infections for which there is no cure. Periodic viral reactivation spreads infection to new hosts while causing various disease states particularly detrimental in the ...
Elizabeth B. Draganova, Ekaterina E. Heldwein   +1 more
doaj   +6 more sources

Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97 [PDF]

Viruses, 2018
The nuclear phase of herpesvirus replication is regulated through the formation of regulatory multi-component protein complexes. Viral genomic replication is followed by nuclear capsid assembly, DNA encapsidation and nuclear egress.
Jens Milbradt, Eric Sonntag, Sabrina Wagner, Hanife Strojan, Christina Wangen, Tihana Lenac Rovis, Berislav Lisnic, Stipan Jonjic, Heinrich Sticht, William J. Britt, Ursula Schlötzer-Schrehardt, Manfred Marschall   +11 more
doaj   +6 more sources

pUL21 regulation of pUs3 kinase activity influences the nature of nuclear envelope deformation by the HSV-2 nuclear egress complex. [PDF]

PLoS Pathogens, 2021
It is well established that the herpesvirus nuclear egress complex (NEC) has an intrinsic ability to deform membranes. During viral infection, the membrane-deformation activity of the NEC must be precisely regulated to ensure efficient nuclear egress of ...
Jamil H Muradov, Renée L Finnen, Michael A Gulak, Thomas J M Hay, Bruce W Banfield   +4 more
doaj   +6 more sources

Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling [PDF]

Cell Reports, 2015
Although nucleo-cytoplasmic transport is typically mediated through nuclear pore complexes, herpesvirus capsids exit the nucleus via a unique vesicular pathway.
Tzviya Zeev-Ben-Mordehai, Marion Weberruß, Michael Lorenz, Juliana Cheleski, Teresa Hellberg, Cathy Whittle, Kamel El Omari, Daven Vasishtan, Kyle C. Dent, Karl Harlos, Kati Franzke, Christoph Hagen, Barbara G. Klupp, Wolfram Antonin, Thomas C. Mettenleiter, Kay Grünewald   +15 more
doaj   +7 more sources

Patterns of Autologous and Nonautologous Interactions between Core Nuclear Egress Complex (NEC) Proteins of α-, β- and γ-Herpesviruses [PDF]

Viruses, 2020
Nuclear egress is a regulated process shared by α-, β- and γ-herpesviruses. The core nuclear egress complex (NEC) is composed of the membrane-anchored protein homologs of human cytomegalovirus (HCMV) pUL50, murine cytomegalovirus (MCMV ...
Sigrun Häge, Eric Sonntag, Eva Maria Borst, Pierre Tannig, Lisa Seyler, Tobias Bäuerle, Susanne M. Bailer, Chung-Pei Lee, Regina Müller, Christina Wangen, Jens Milbradt, Manfred Marschall   +11 more
doaj   +8 more sources

Properties of Oligomeric Interaction of the Cytomegalovirus Core Nuclear Egress Complex (NEC) and Its Sensitivity to an NEC Inhibitory Small Molecule [PDF]

Viruses, 2021
Herpesviral nuclear egress is a regulated process shared by all family members, ensuring the efficient cytoplasmic release of viral capsids. In the case of human cytomegalovirus (HCMV), the core of the nuclear egress complex (NEC) consists of the pUL50 ...
Jintawee Kicuntod, Sewar Alkhashrom, Sigrun Häge, Benedikt Diewald, Regina Müller, Friedrich Hahn, Peter Lischka, Heinrich Sticht, Jutta Eichler, Manfred Marschall   +9 more
doaj   +2 more sources

The universal suppressor mutation restores membrane budding defects in the HSV-1 nuclear egress complex by stabilizing the oligomeric lattice. [PDF]

PLoS Pathogens
Nuclear egress is an essential process in herpesvirus replication whereby nascent capsids translocate from the nucleus to the cytoplasm. This initial step of nuclear egress-budding at the inner nuclear membrane-is coordinated by the nuclear egress ...
Elizabeth B Draganova, Hui Wang, Melanie Wu, Shiqing Liao, Amber Vu, Gonzalo L Gonzalez-Del Pino, Z Hong Zhou, Richard J Roller, Ekaterina E Heldwein   +8 more
doaj   +2 more sources

The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus [PDF]

Cells, 2021
The regulation of the nucleocytoplasmic release of herpesviral capsids is defined by the process of nuclear egress. Due to their large size, nuclear capsids are unable to traverse via nuclear pores, so that herpesviruses evolved to develop a vesicular ...
Sigrun Häge, Nicole Büscher, Victoria Pakulska, Friedrich Hahn, Annie Adrait, Steffi Krauter, Eva Maria Borst, Ursula Schlötzer-Schrehardt, Yohann Couté, Bodo Plachter, Manfred Marschall   +10 more
doaj   +5 more sources

The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding [PDF]

Scientific Reports, 2017
The herpes simplex virus (HSV) UL31 gene encodes a conserved member of the herpesvirus nuclear egress complex that not only functions in the egress of DNA containing capsids from the nucleus, but is also required for optimal replication of viral DNA and ...
Maxwell R. Sherry, Thomas J. M. Hay, Michael A. Gulak, Arash Nassiri, Renée L. Finnen, Bruce W. Banfield   +5 more
doaj   +2 more sources