Results 21 to 30 of about 10,967 (201)

Highly Basic Clusters in the Herpes Simplex Virus 1 Nuclear Egress Complex Drive Membrane Budding by Inducing Lipid Ordering [PDF]

mBio, 2021
During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This unusual process is mediated by the viral nuclear egress complex (NEC) that deforms the membrane around the capsid by ...
Michael K. Thorsen, Alex Lai, Michelle W. Lee, David P. Hoogerheide, Gerard C. L. Wong, Jack H. Freed, Ekaterina E. Heldwein   +6 more
doaj   +2 more sources

The HSV1 Tail-Anchored Membrane Protein pUL34 Contains a Basic Motif That Supports Active Transport to the Inner Nuclear Membrane Prior to Formation of the Nuclear Egress Complex [PDF]

Viruses, 2021
Herpes simplex virus type 1 nucleocapsids are released from the host nucleus by a budding process through the nuclear envelope called nuclear egress. Two viral proteins, the integral membrane proteins pUL34 and pUL31, form the nuclear egress complex at ...
Christina Funk, Débora Marques da Silveira e Santos, Melanie Ott, Verena Raschbichler, Susanne M. Bailer   +4 more
doaj   +2 more sources

Host and Viral Factors Involved in Nuclear Egress of Herpes Simplex Virus 1

Viruses, 2021
Herpes simplex virus 1 (HSV-1) replicates its genome and packages it into capsids within the nucleus. HSV-1 has evolved a complex mechanism of nuclear egress whereby nascent capsids bud on the inner nuclear membrane to form perinuclear virions that ...
Jun Arii
doaj   +3 more sources

Patterns of Autologous and Nonautologous Interactions between Core Nuclear Egress Complex (NEC) Proteins of α-, β- and γ-Herpesviruses [PDF]

Viruses, 2020
Nuclear egress is a regulated process shared by α-, β- and γ-herpesviruses. The core nuclear egress complex (NEC) is composed of the membrane-anchored protein homologs of human cytomegalovirus (HCMV) pUL50, murine cytomegalovirus (MCMV ...
Sigrun Häge, Eric Sonntag, Eva Maria Borst, Pierre Tannig, Lisa Seyler, Tobias Bäuerle, Susanne M. Bailer, Chung-Pei Lee, Regina Müller, Christina Wangen, Jens Milbradt, Manfred Marschall   +11 more
doaj   +2 more sources

The universal suppressor mutation restores membrane budding defects in the HSV-1 nuclear egress complex by stabilizing the oligomeric lattice. [PDF]

PLoS Pathogens
Nuclear egress is an essential process in herpesvirus replication whereby nascent capsids translocate from the nucleus to the cytoplasm. This initial step of nuclear egress-budding at the inner nuclear membrane-is coordinated by the nuclear egress ...
Elizabeth B Draganova, Hui Wang, Melanie Wu, Shiqing Liao, Amber Vu, Gonzalo L Gonzalez-Del Pino, Z Hong Zhou, Richard J Roller, Ekaterina E Heldwein   +8 more
doaj   +2 more sources

The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding [PDF]

Scientific Reports, 2017
The herpes simplex virus (HSV) UL31 gene encodes a conserved member of the herpesvirus nuclear egress complex that not only functions in the egress of DNA containing capsids from the nucleus, but is also required for optimal replication of viral DNA and ...
Maxwell R. Sherry, Thomas J. M. Hay, Michael A. Gulak, Arash Nassiri, Renée L. Finnen, Bruce W. Banfield   +5 more
doaj   +2 more sources

GRWD1 enhances HSV-1 replication by facilitating nuclear egress [PDF]

Microbiology Spectrum
Herpes simplex virus 1 (HSV-1) depends on host factors for its replication. Here, we identify glutamate-rich WD40 repeat-containing 1 (GRWD1) as a novel pro-viral host factor that facilitates HSV-1 nuclear egress. GRWD1 knockdown trapped nucleocapsids in
Zimeng Kong, Chao Gao, Xingyun Liu, Teng Xue, Xinxin Liang, Peijie Yan, Rui Zhang, Jun Tang   +7 more
doaj   +2 more sources

Proteomic analysis of the multimeric nuclear egress complex of human cytomegalovirus. [PDF]

Mol Cell Proteomics, 2014
Herpesviral capsids are assembled in the host cell nucleus before being translocated into the cytoplasm for further maturation. The crossing of the nuclear envelope represents a major event that requires the formation of the nuclear egress complex (NEC).
Milbradt J, Kraut A, Hutterer C, Sonntag E, Schmeiser C, Ferro M, Wagner S, Lenac T, Claus C, Pinkert S, Hamilton ST, Rawlinson WD, Sticht H, Couté Y, Marschall M.   +14 more
europepmc   +6 more sources

NECing goes: flexibility of the herpesvirus nuclear egress complex

, 2023
Abstract The nuclear egress complex (NEC) allows herpesvirus capsids to escape from the nucleus without breaking the nuclear envelope barrier. It assembles into a lattice on the inner nuclear membrane enveloping newly assembled nucleocapsids, which bud into the perinuclear space.
Pražák V, Mironova Y, Vasishtan D, Hagen C, Laugks U, Jensen Y, Sanders S, Heumann JM, Bosse JB, Klupp B, Mettenleiter TC, Grange M, Grünewald K.   +12 more
europepmc   +2 more sources

Human cytomegalovirus UL97 phosphorylates the viral nuclear egress complex. [PDF]

J Virol, 2015
ABSTRACT Herpesvirus nucleocapsids exit the host cell nucleus in an unusual process known as nuclear egress. The human cytomegalovirus (HCMV) UL97 protein kinase is required for efficient nuclear egress, which can be explained by its phosphorylation of the nuclear lamina component lamin A/C, which disrupts the nuclear lamina.
Sharma M, Bender BJ, Kamil JP, Lye MF, Pesola JM, Reim NI, Hogle JM, Coen DM.   +7 more
europepmc   +6 more sources