Expression, Purification and Crystallization of the Herpesvirus Nuclear Egress Complex (NEC) [PDF]
Bio-Protocol, 2016 The protocol describes the production and crystallization of the soluble form of the nuclear egress complex (NEC) from Herpes simplex virus 1 and Pseudorabies virus. The NEC is a heterodimer that consists of conserved proteins UL31 and UL34.
Janna Bigalke, Ekaterina Heldwein
doaj +5 more sources
pUL21 regulation of pUs3 kinase activity influences the nature of nuclear envelope deformation by the HSV-2 nuclear egress complex. [PDF]
PLoS Pathogens, 2021 It is well established that the herpesvirus nuclear egress complex (NEC) has an intrinsic ability to deform membranes. During viral infection, the membrane-deformation activity of the NEC must be precisely regulated to ensure efficient nuclear egress of ...
Jamil H Muradov +4 more
doaj +6 more sources
Virus-derived peptide inhibitors of the herpes simplex virus type 1 nuclear egress complex [PDF]
Scientific Reports, 2021 Herpesviruses infect a majority of the human population, establishing lifelong latent infections for which there is no cure. Periodic viral reactivation spreads infection to new hosts while causing various disease states particularly detrimental in the ...
Elizabeth B. Draganova +1 more
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The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus [PDF]
Cells, 2021 The regulation of the nucleocytoplasmic release of herpesviral capsids is defined by the process of nuclear egress. Due to their large size, nuclear capsids are unable to traverse via nuclear pores, so that herpesviruses evolved to develop a vesicular ...
Sigrun Häge +10 more
doaj +7 more sources
Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling [PDF]
Cell Reports, 2015 Although nucleo-cytoplasmic transport is typically mediated through nuclear pore complexes, herpesvirus capsids exit the nucleus via a unique vesicular pathway.
Tzviya Zeev-Ben-Mordehai +15 more
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Functional Identification and Characterization of the Nuclear Egress Complex of a Gammaherpesvirus. [PDF]
J Virol, 2019 Increasing amounts of knowledge indicate that the nuclear egress complex (NEC) is critical for the nuclear egress of herpesvirus capsids, which can be viewed as a vesicle-mediated transport pathway through the nuclear membrane. In this study, we identified open reading frame 67 (ORF67) and ORF69 as components of the NEC in murine gammaherpesvirus 68 ...
Lv Y +6 more
europepmc +5 more sources
Mutational Functional Analysis of the Pseudorabies Virus Nuclear Egress Complex-Nucleocapsid Interaction. [PDF]
J Virol, 2020 Herpesviruses encode an exceptional vesicle formation and scission machinery, which operates at the inner nuclear membrane, translocating the viral nucleocapsid from the nucleus into the perinuclear space. The conserved herpesviral nuclear egress complex (NEC) orchestrates this process.
Rönfeldt S +4 more
europepmc +5 more sources
Structural basis of membrane budding by the nuclear egress complex of herpesviruses. [PDF]
EMBO J, 2015 During nuclear egress, herpesvirus capsids bud at the inner nuclear membrane forming perinuclear viral particles that subsequently fuse with the outer nuclear membrane, releasing capsids into the cytoplasm. This unusual budding process is mediated by the nuclear egress complex (NEC) composed of two conserved viral proteins, UL31 and UL34.
Bigalke JM, Heldwein EE.
europepmc +4 more sources
Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins. [PDF]
Methods Enzymol, 2016 Herpesviruses, like most DNA viruses, replicate their genomes in the host cell nucleus. Their DNA is then packaged and assembled into viral nucleocapsids, which, in most cases, are too large to pass through the nuclear pore complex. Instead, herpesviruses use a complex multistep pathway, termed nuclear egress, to exit the nucleus.
Sharma M, Kamil JP, Coen DM.
europepmc +5 more sources
Unexpected features and mechanism of heterodimer formation of a herpesvirus nuclear egress complex. [PDF]
EMBO J, 2015 Herpesvirus nucleocapsids escape from the nucleus in a process orchestrated by a highly conserved, viral nuclear egress complex. In human cytomegalovirus, the complex consists of two proteins, UL50 and UL53. We solved structures of versions of UL53 and the complex by X-ray crystallography.
Lye MF +6 more
europepmc +5 more sources