Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97 [PDF]
Viruses, 2018 The nuclear phase of herpesvirus replication is regulated through the formation of regulatory multi-component protein complexes. Viral genomic replication is followed by nuclear capsid assembly, DNA encapsidation and nuclear egress.
Jens Milbradt +11 more
doaj +8 more sources
Expression, Purification and Crystallization of the Herpesvirus Nuclear Egress Complex (NEC) [PDF]
Bio-Protocol, 2016 The protocol describes the production and crystallization of the soluble form of the nuclear egress complex (NEC) from Herpes simplex virus 1 and Pseudorabies virus. The NEC is a heterodimer that consists of conserved proteins UL31 and UL34.
Janna Bigalke, Ekaterina Heldwein
doaj +5 more sources
Virus-derived peptide inhibitors of the herpes simplex virus type 1 nuclear egress complex [PDF]
Scientific Reports, 2021 Herpesviruses infect a majority of the human population, establishing lifelong latent infections for which there is no cure. Periodic viral reactivation spreads infection to new hosts while causing various disease states particularly detrimental in the ...
Elizabeth B. Draganova +1 more
doaj +6 more sources
pUL21 regulation of pUs3 kinase activity influences the nature of nuclear envelope deformation by the HSV-2 nuclear egress complex. [PDF]
PLoS Pathogens, 2021 It is well established that the herpesvirus nuclear egress complex (NEC) has an intrinsic ability to deform membranes. During viral infection, the membrane-deformation activity of the NEC must be precisely regulated to ensure efficient nuclear egress of ...
Jamil H Muradov +4 more
doaj +6 more sources
Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling [PDF]
Cell Reports, 2015 Although nucleo-cytoplasmic transport is typically mediated through nuclear pore complexes, herpesvirus capsids exit the nucleus via a unique vesicular pathway.
Tzviya Zeev-Ben-Mordehai +15 more
doaj +7 more sources
Properties of Oligomeric Interaction of the Cytomegalovirus Core Nuclear Egress Complex (NEC) and Its Sensitivity to an NEC Inhibitory Small Molecule [PDF]
Viruses, 2021 Herpesviral nuclear egress is a regulated process shared by all family members, ensuring the efficient cytoplasmic release of viral capsids. In the case of human cytomegalovirus (HCMV), the core of the nuclear egress complex (NEC) consists of the pUL50 ...
Jintawee Kicuntod +9 more
doaj +4 more sources
The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus [PDF]
Cells, 2021 The regulation of the nucleocytoplasmic release of herpesviral capsids is defined by the process of nuclear egress. Due to their large size, nuclear capsids are unable to traverse via nuclear pores, so that herpesviruses evolved to develop a vesicular ...
Sigrun Häge +10 more
doaj +7 more sources
Patterns of Autologous and Nonautologous Interactions between Core Nuclear Egress Complex (NEC) Proteins of α-, β- and γ-Herpesviruses [PDF]
Viruses, 2020 Nuclear egress is a regulated process shared by α-, β- and γ-herpesviruses. The core nuclear egress complex (NEC) is composed of the membrane-anchored protein homologs of human cytomegalovirus (HCMV) pUL50, murine cytomegalovirus (MCMV ...
Sigrun Häge +11 more
doaj +8 more sources
Functional Identification and Characterization of the Nuclear Egress Complex of a Gammaherpesvirus. [PDF]
J Virol, 2019 Increasing amounts of knowledge indicate that the nuclear egress complex (NEC) is critical for the nuclear egress of herpesvirus capsids, which can be viewed as a vesicle-mediated transport pathway through the nuclear membrane. In this study, we identified open reading frame 67 (ORF67) and ORF69 as components of the NEC in murine gammaherpesvirus 68 ...
Lv Y +6 more
europepmc +6 more sources
Structural basis of membrane budding by the nuclear egress complex of herpesviruses. [PDF]
EMBO J, 2015 AbstractDuring nuclear egress, herpesvirus capsids bud at the inner nuclear membrane forming perinuclear viral particles that subsequently fuse with the outer nuclear membrane, releasing capsids into the cytoplasm. This unusual budding process is mediated by the nuclear egress complex (NEC) composed of two conserved viral proteins, UL31 and UL34 ...
Bigalke JM, Heldwein EE.
europepmc +5 more sources