The Primary Enveloped Virion of Herpes Simplex Virus 1: Its Role in Nuclear Egress
mBio, 2017 Many viruses migrate between different cellular compartments for successive stages of assembly. The HSV-1 capsid assembles in the nucleus and then transfers into the cytoplasm.
William W. Newcomb +5 more
doaj +3 more sources
Function of Torsin AAA+ ATPases in Pseudorabies Virus Nuclear Egress [PDF]
Cells, 2020 Newly assembled herpesvirus nucleocapsids traverse the intact nuclear envelope by a vesicle-mediated nucleo-cytoplasmic transport for final virion maturation in the cytoplasm.
Julia E. Hölper +4 more
doaj +2 more sources
The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding [PDF]
Scientific Reports, 2017 The herpes simplex virus (HSV) UL31 gene encodes a conserved member of the herpesvirus nuclear egress complex that not only functions in the egress of DNA containing capsids from the nucleus, but is also required for optimal replication of viral DNA and ...
Maxwell R. Sherry +5 more
doaj +2 more sources
GRWD1 enhances HSV-1 replication by facilitating nuclear egress [PDF]
Microbiology SpectrumHerpes simplex virus 1 (HSV-1) depends on host factors for its replication. Here, we identify glutamate-rich WD40 repeat-containing 1 (GRWD1) as a novel pro-viral host factor that facilitates HSV-1 nuclear egress. GRWD1 knockdown trapped nucleocapsids in
Zimeng Kong +7 more
doaj +2 more sources
Cells, 2022 Herpesviruses replicate their genomes and assemble their capsids in the host cell nucleus. To progress towards morphogenesis in the cytoplasm, herpesviruses evolved the strategy of nuclear egress as a highly regulated process of nucleo-cytoplasmic capsid
Josephine Lösing +6 more
doaj +3 more sources
Identification of conserved amino acids in pUL34 which are critical for function of the pseudorabies virus nuclear egress complex. [PDF]
J Virol, 2014 Nuclear egress of herpesvirus capsids is mediated by a conserved heterodimeric complex of two viral proteins, designated pUL34 and pUL31 in herpes simplex virus and pseudorabies virus (PrV).
Paßvogel L +4 more
europepmc +5 more sources
Reconstitution of the Kaposi's Sarcoma-Associated Herpesvirus Nuclear Egress Complex and Formation of Nuclear Membrane Vesicles by Coexpression of ORF67 and ORF69 Gene Products [PDF]
Journal of Virology, 2012 The Kaposi's sarcoma-associated herpesvirus nuclear egress complex is composed of two proteins, ORF67 and ORF69. In this study, we have recapitulated the KSHV complex by coexpression of these two proteins in insect cells using expression from recombinant
Eric M. Luitweiler +4 more
core +3 more sources
Viruses and Nuclear Egress [PDF]
, 2022 This Special Issue of Viruses focuses on the topic of viruses and nuclear egress. Nuclear egress is a fascinating process by which herpesvirus nucleocapsids make their way from the nuclear interior to the cytoplasm.
core +2 more sources
Proteomic analysis of the multimeric nuclear egress complex of human cytomegalovirus. [PDF]
Mol Cell Proteomics, 2014 Herpesviral capsids are assembled in the host cell nucleus before being translocated into the cytoplasm for further maturation. The crossing of the nuclear envelope represents a major event that requires the formation of the nuclear egress complex (NEC).
Milbradt J +14 more
europepmc +6 more sources
Human cytomegalovirus UL97 phosphorylates the viral nuclear egress complex. [PDF]
J Virol, 2015 ABSTRACT Herpesvirus nucleocapsids exit the host cell nucleus in an unusual process known as nuclear egress. The human cytomegalovirus (HCMV) UL97 protein kinase is required for efficient nuclear egress, which can be explained by its phosphorylation of the nuclear lamina component lamin A/C, which disrupts the nuclear lamina.
Sharma M +7 more
europepmc +6 more sources