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Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance
Journal of Agricultural and Food Chemistry, 2021An immunoassay is mostly employed for the direct detection of food contaminants, and a molecular assay for targeting nucleic acids employs amplification techniques for distinguishing genes. The integration of an immunoassay with nucleic acid amplification techniques inherits the direct and rapid performance of an immunoassay and the ultrasensitive ...
Yuliang Cheng, Hang Yu, Yunfei Xie
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Bioanalytical applications of isothermal nucleic acid amplification techniques
Analytica Chimica Acta, 2015The most popular in vitro nucleic acid amplification techniques like polymerase chain reaction (PCR) including real-time PCR are costly and require thermocycling, rendering them unsuitable for uses at point-of-care. Highly efficient in vitro nucleic acid amplification techniques using simple, portable and low-cost instruments are crucial in disease ...
Huimin Deng, Zhiqiang Gao
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Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing
Talanta, 2021 Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
Idorenyin A. Iwe +3 more
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Biosensors based on functional nucleic acids and isothermal amplification techniques
Talanta, 2023In the past few years, with the in-depth research of functional nucleic acids and isothermal amplification techniques, their applications in the field of biosensing have attracted great interest. Since functional nucleic acids have excellent flexibility and convenience in their structural design, they have significant advantages as recognition elements
Qubo Zhu
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Mycoses, 2020
AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Thales Domingos Arantes +1 more
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AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Thales Domingos Arantes +1 more
exaly +3 more sources
Hairpin DNA-mediated isothermal amplification techniques for nucleic acid testing
, 2021 Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
Iwe, Idorenyin Asuquo
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Analytical Biochemistry, 2020
The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry ...
Omar Mukama +2 more
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The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry ...
Omar Mukama +2 more
exaly +3 more sources
Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques
Chinese Journal of Analytical Chemistry, 2016Abstract Recently, micro/nano-scaled fluidic control technologies have been developed to be the alternative to traditional analysis approaches due to the capability of realizing miniaturized multiphase and multistep reactions. The perfect combination of these techniques and nucleic acid amplification methods effectively promotes the establishment and
Xiong Ding, Ying Mu
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Nucleic acid-aided molecular amplification techniques for food microorganism detection
TrAC - Trends in Analytical Chemistry, 2023Xinyue Lan, Longjiao Zhu, Haiyan Liu
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