Results 251 to 260 of about 78,833 (285)

Chemiluminescent sensors based on nucleic acid amplification techniques and functional nucleic acids

Journal of Luminescence
Yaru Liang, Xu Teng
exaly   +2 more sources

A netlike rolling circle nucleic acid amplification technique

The Analyst, 2015
An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.
Xiaoli, Zhu, Chang, Feng, Bin, Zhang, Hui, Tong, Tao, Gao, Genxi, Li   +5 more
openaire   +2 more sources

Validation criteria for nucleic acid amplification techniques for bacterial infections

Clinical Chemistry and Laboratory Medicine, 2008
Nucleic acid techniques (NATs), such as species-specific and universal polymerase chain reactions (PCRs), are finding ever wider use in the diagnosis of bacterial infection. However, although universal PCR assays, in particular, approach a type of modern Petri dish, they have a number of limitations which restrict their applicability.
Gerd, Döring, Klaus, Unertl, Alexandra, Heininger   +2 more
openaire   +2 more sources

New nucleic acid amplification techniques in diagnostic pathology

Journal of the European Academy of Dermatology and Venereology, 1994
AbstractIn 1985, Mullis discovered an exponentially increasing amplification system for nucleic acids, which became known as the polymerase chain reaction (PCR). This method permits the selective in vitro amplification of a particular DNA region by mimicking phenomena of in vivo DNA replication.
J.J. O'Leary, G. Browne, R. Landers, M. Crowley, I. Healy, M.I. Johnson, C.T. Doyle   +6 more
openaire   +1 more source

Nucleic acid amplification and sequencing techniques

1993
While nucleic acid hybridization procedures have now developed to the extent that they can be used readily for identification and typing of a wide range of microorganisms, the target nucleic acid is available only in very limited quantities unless the organism is first cultivated. Culture methods have not, however, been devised for some microorganisms,
K. J. Towner, A. Cockayne
openaire   +1 more source

Concordance between nucleic acid amplification technique and culture for the diagnosis of gonorrhoea

International Journal of STD & AIDS, 2009
The objective of the study was to evaluate the concordance between nucleic acid amplification technique (NAAT) and culture for the diagnosis of Neisseria gonorrhoeae among attendees at a genitourinary medicine clinic in East London. All patients testing positive for N.
S, Creighton, B, Revell, A, Barrow
openaire   +2 more sources

An Introduction to In Vitro Nucleic Acid Amplification Techniques

2012
A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and optimization of a series of in vitro nucleic acid amplification (NAA) technologies has opened new avenues in diagnostic microbiology for the ...
Haijing Li, Yi-Wei Tang
openaire   +1 more source

Isothermal Amplification Techniques for the Detection of Nucleic Acids and Proteins

2020
The early detection of disease is beneficial for improved patient prognoses. One major challenge in early disease detection is the small, undetectable amounts of biomarkers. Detection strategies that confer amplification of the biomarker itself or of the detection signal are therefore desirable. Furthermore, emerging strategies conferring amplification
openaire   +1 more source

Amplification-Based Nucleic Acid Scanning Techniques to Assess Genetic Polymorphism inCandida

Critical Reviews in Microbiology, 2003
Opportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C.
Dassanayake, RS, Samaranayake, LP
openaire   +6 more sources

Quantitative Reverse Transcription Strand Displacement Amplification: Quantitation of Nucleic Acids Using an Isothermal Amplification Technique

Analytical Biochemistry, 1998
Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent testing is carried out for HIV viral load. Strand displacement amplification (SDA) is an isothermal DNA amplification system utilizing a restriction enzyme and a DNA polymerase with strand ...
C M, Nycz, C H, Dean, P D, Haaland, C A, Spargo, G T, Walker   +4 more
openaire   +2 more sources