Results 261 to 270 of about 187,051 (303)

New nucleic acid amplification techniques in diagnostic pathology

Journal of the European Academy of Dermatology and Venereology, 1994
AbstractIn 1985, Mullis discovered an exponentially increasing amplification system for nucleic acids, which became known as the polymerase chain reaction (PCR). This method permits the selective in vitro amplification of a particular DNA region by mimicking phenomena of in vivo DNA replication.
J.J. O'Leary, G. Browne, R. Landers, M. Crowley, I. Healy, M.I. Johnson, C.T. Doyle   +6 more
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Validation criteria for nucleic acid amplification techniques for bacterial infections

Clinical Chemistry and Laboratory Medicine, 2008
Nucleic acid techniques (NATs), such as species-specific and universal polymerase chain reactions (PCRs), are finding ever wider use in the diagnosis of bacterial infection. However, although universal PCR assays, in particular, approach a type of modern Petri dish, they have a number of limitations which restrict their applicability.
Gerd, Döring, Klaus, Unertl, Alexandra, Heininger   +2 more
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Nucleic acid amplification and sequencing techniques

1993
While nucleic acid hybridization procedures have now developed to the extent that they can be used readily for identification and typing of a wide range of microorganisms, the target nucleic acid is available only in very limited quantities unless the organism is first cultivated. Culture methods have not, however, been devised for some microorganisms,
K. J. Towner, A. Cockayne
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Quantitative Reverse Transcription Strand Displacement Amplification: Quantitation of Nucleic Acids Using an Isothermal Amplification Technique

Analytical Biochemistry, 1998
Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent testing is carried out for HIV viral load. Strand displacement amplification (SDA) is an isothermal DNA amplification system utilizing a restriction enzyme and a DNA polymerase with strand ...
C M, Nycz, C H, Dean, P D, Haaland, C A, Spargo, G T, Walker   +4 more
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Hairpin DNA-mediated isothermal amplification techniques for nucleic acid testing

2021
Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
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Amplification-Based Nucleic Acid Scanning Techniques to Assess Genetic Polymorphism inCandida

Critical Reviews in Microbiology, 2003
Opportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C.
Dassanayake, RS, Samaranayake, LP
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An Introduction to In Vitro Nucleic Acid Amplification Techniques

2012
A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and optimization of a series of in vitro nucleic acid amplification (NAA) technologies has opened new avenues in diagnostic microbiology for the ...
Haijing Li, Yi-Wei Tang
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Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques

Chinese Journal of Analytical Chemistry, 2016
Abstract Recently, micro/nano-scaled fluidic control technologies have been developed to be the alternative to traditional analysis approaches due to the capability of realizing miniaturized multiphase and multistep reactions. The perfect combination of these techniques and nucleic acid amplification methods effectively promotes the establishment and
Xiong DING, Ying MU
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Concordance between nucleic acid amplification technique and culture for the diagnosis of gonorrhoea

International Journal of STD & AIDS, 2009
The objective of the study was to evaluate the concordance between nucleic acid amplification technique (NAAT) and culture for the diagnosis of Neisseria gonorrhoeae among attendees at a genitourinary medicine clinic in East London. All patients testing positive for N.
S, Creighton, B, Revell, A, Barrow
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Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics

Analytical Biochemistry, 2020
The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry ...
Omar, Mukama, Chengrong, Nie, Jean de Dieu, Habimana, Xiaogao, Meng, Yuan, Ting, Fanuel, Songwe, Ammar, Al Farga, Samson, Mugisha, Peter, Rwibasira, Yinghui, Zhang, Lingwen, Zeng   +10 more
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