Results 1 to 10 of about 4,329 (140)

Retroviral nucleocapsid proteins possess potent nucleic acid strand renaturation activity [PDF]

Protein Science, 1993
AbstractThe nucleocapsid protein (NC) is the major genomic RNA binding protein that plays integral roles in the structure and replication of all animal retroviruses. In this report, select biochemical properties of recombinant Mason‐Pfizer monkey virus (MPMV) and HIV‐1 NCs are compared.
David P Giedroc
exaly   +4 more sources

Studies on nucleic acid reassociation kinetics: rate of hybridization of excess RNA with DNA, compared to the rate of DNA renaturation. [PDF]

Proceedings of the National Academy of Sciences, 1977
The rate of reaction of double-stranded replicative form (RF) [3H]DNA of bacteriophage phiX174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA-DNA reaction differs less than 25% from that of the DNA ...
Galau, Glenn A., Britten, Roy J., Davidson, Eric H.   +2 more
openaire   +4 more sources

Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation. [PDF]

Proceedings of the National Academy of Sciences, 1975
The reassociation kinetics of Escherichia coli DNA were measured by S1 nuclease resistance and hydroxyapatite binding. While the reaction assayed by hydroxyapatite displays second order kinetics, the S1 nuclease measurements follow a non-second order from, as previously reported by Morrow (Ph.D. Dissertation, Stanford University. 1974).
Smith, Michael J., Britten, Roy J., Davidson, Eric H.   +2 more
openaire   +4 more sources

Microarray results improve significantly as hybridization approaches equilibrium

BioTechniques, 2004
Dual-channel long oligonucleotide microarrays are in widespread use. Although much attention has been given to proper experimental design and analysis regarding long oligonucleotide microarrays, relatively little information is available concerning the ...
Maureen Sartor, Jennifer Schwanekamp, Danielle Halbleib, Ismail Mohamed, Saikumar Karyala, Mario Medvedovic, Craig R. Tomlinson   +6 more
doaj   +2 more sources

Production of recombinant norovirus VP1 protein and its antigenic and immunogenic properties

Журнал микробиологии, эпидемиологии и иммунобиологии
Introduction. The importance of noroviruses in human infectious pathology and the danger of large epidemic outbreaks in organized groups determine the need to develop means of specific prevention of infection.
Vladislav A. Lapin, Dmitry V. Novikov, Ekaterina V. Mokhonova, Dmitry A. Melentyev, Maria I. Tsyganova, Dmitry E. Zaitsev, Viktor V. Novikov   +6 more
doaj   +2 more sources

Mechanism of Thermal Renaturation and Hybridization of Nucleic Acids: Kramers’ Process and Universality in Watson−Crick Base Pairing [PDF]

The Journal of Physical Chemistry B, 2009
Renaturation and hybridization reactions lead to the pairing of complementary single-stranded nucleic acids. We present here a theoretical investigation of the mechanism of these reactions in vitro under thermal conditions (dilute solutions of single-stranded chains, in the presence of molar concentrations of monovalent salts and at elevated ...
Sikorav, Jean-Louis, Orland, Henri, Braslau, Alan   +2 more
openaire   +3 more sources

Nucleic acid renaturation and restriction endonuclease cleavage analyses show that the DNAs of a transforming and a nontransforming strain of Epstein-Barr virus share approximately 90% of their nucleotide sequences [PDF]

Journal of Virology, 1976
Viral DNA molecules were purified from a nontransforming and a transforming strain of Epstein-Barr virus. Each viral DNA was labeled in vitro and renatured in the presence of an excess of either one or the other unlabeled viral DNA. Both viral DNAs were also digested with the Eco R1 restriction endonuclease and subsequently labeled by using avian ...
B, Sugden, W C, Summers, G, Klein
openaire   +2 more sources

Programmable DNA Folding Modulates Phase Behavior and Dynamics of DNA/Peptide Condensates. [PDF]

ACS Nano
Katzir I, Wen Y, Razi I, Skoi V, Beck R, Dong H, Lampel A.   +6 more
europepmc   +1 more source

High-throughput measurement and prediction of the i-motif DNA stability landscape. [PDF]

Nucleic Acids Res
Chu C, Zhang S, Guan Z, Shen J, Luo T, Zhou J, Mergny JL, Cheng M.   +7 more
europepmc   +1 more source

Impact of synonymous mutations in the <i>bla</i>_TEM-3 gene on gene expression and <i>Escherichia coli</i> fitness. [PDF]

Microbiol Spectr
Dhungana R, Kaadan H, Paudel A, Oelschlaeger P.   +3 more
europepmc   +1 more source