β-1,3 Glucan Microparticles & Nanoparticles: Fabrication Methods & Applications in Immunomodulation & Targeted Drug Delivery. [PDF]
Adv Healthc MaterBeta‐glucans have gained significant attention as an immunomodulatory biomaterial with cell‐targeting capabilities. This review comprehensively outlines the design and fabrication methods for producing beta‐glucan particles at both the micro and nanoscale and their applications in immune cell targeting, immunomodulation, and drug delivery ...
Dowdall N, Hoare T.
europepmc +2 more sources
An efficient strategy for producing RNA-free Nucleocapsid protein of SARS-CoV-2 for biochemical and structural investigations. [PDF]
FEBS Open BioCleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Singh S, Gupta GD.
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Development and Validation of a Three-Step Screening Strategy for Extracellular Salt-Tolerant Nucleases From Marine Bacteria. [PDF]
Biotechnol JGraphical Abstract and Lay Summary A three‐step screen for quick discovery of extracellular salt‐active/tolerant nucleases from halophiles–a Biotechnological Gold Mine. ABSTRACT This study reports the development of a 3‐step strategy that is both cost‐effective and quick to screen marine organisms and validate the presence of extracellular nucleases ...
Achar A, Sen S.
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Stochastic Simulation of the Kinetics of Multiple Interacting Nucleic Acid Strands [PDF]
, 2013 DNA nanotechnology is an emerging field which utilizes the unique structural properties of nucleic acids in order to build nanoscale devices, such as logic gates, motors, walkers, and algorithmic structures. Predicting the structure and interactions of a
Schaeffer, Joseph Malcolm
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Pseudovibrio axinellae sp. nov., isolated from an Irish marine sponge [PDF]
, 2012 A Gram-negative, motile, rod-shaped bacterial strain, designated Ad2T, was isolated from a marine sponge, Axinella dissimilis, which was collected from a semi-enclosed marine lake in Ireland.
Teresa M. Barbosa +5 more
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Accurate quantification of nucleic acids using hypochromicity measurements in conjunction with UV spectrophotometry [PDF]
, 2017 UV absorbance spectrophotometry is widely used for the quantification of nucleic acids. For accurate quantification it is important to determine the hypochromocity of the oligonucleotide or complex nucleic acid structure.
David E. Portwood +7 more
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Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
, 2010 The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
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Unique and conserved MicroRNAs in wheat chromosome 5D revealed by next-generation sequencing [PDF]
, 2013 MicroRNAs are a class of short, non-coding, single-stranded RNAs that act as post-transcriptional regulators in gene expression. miRNA analysis of Triticum aestivum chromosome 5D was performed on 454 GS FLX Titanium sequences of flow sorted chromosome 5D
Hikmet Budak (150694) +15 more
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, 1967 The contents of nucleic acids in rat liver and hepatoma mitochondria and the physico-chemical properties on DNA's isolated from these mitochondria were comparatively investigated. The results are briefly summarized as follows. 1.
Inaba, Kozo
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, 2009 A hydrogenotrophic, sulfate-reducing bacterium, designated strain SB1(T), was isolated from sulfidogenic sludge of a full-scale synthesis-gas-fed bioreactor used to remediate wastewater from a zinc smelter. Strain SB1(T) was found to be an abundant micro-
Houten, B.H.G.W., van +5 more
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