Results 21 to 30 of about 4,329 (140)
Techniques to Detect and Quantify the Bacterial Metalloprotease AprX in Bovine Milk: A Review
Comprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 2, March 2026.ABSTRACT The heat‐stable metalloprotease AprX, secreted by psychrotrophic Pseudomonas spp., is a major cause of quality deterioration in dairy products, particularly ultrahigh temperature (UHT) milk. This review synthesizes the evolution and current state of detection and quantification techniques for AprX in bovine milk, covering traditional ...
Aritra Sinha, Alan L. Kelly
wiley +1 more source
The FEBS Journal, Volume 293, Issue 3, Page 781-805, February 2026.Expression of the LEA‐K peptide in E. coli BL21 (DE3) improved bacterial viability under acidic stress (pH4), suggesting a protective function. RNA‐sequencing of LEA‐K‐expressing vs. non‐expressing cells revealed 283 differentially expressed genes (DEGs). In silico docking linked LEA‐K to two‐component systems (TCS) proteins (Rcs, BtsS/R, and EnvZ/OmpR)
Khaled Metwally, Shinya Ikeno
wiley +1 more source
, 2016 Hybridization of complementary nucleic acid strands is fundamental to nearly all molecular bioanalytical methods ranging from polymerase chain reaction and DNA biosensors to next generation sequencing.
Andrew R. Mount (1718773) +5 more
core +1 more source
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
FEBS Open Bio, Volume 16, Issue 1, Page 25-40, January 2026.We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
Oligopyrenotides: Abiotic, Polyanionic Oligomers with Nucleic Acid-like Structural Properties
, 2016 Oligopyrenotides, abiotic oligomers that exhibit significant structural analogies to the nucleic acids, are described. They are composed of achiral, phosphodiester-linked pyrene building blocks and a single chiral 1,2-diaminocyclohexane unit.
Florian Garo (317037) +3 more
core +1 more source
Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials
Advanced Healthcare Materials, Volume 15, Issue 3, 19 January 2026.The production of recombinant proteins represents a fundamental step in the characterisation of marine invertebrate adhesives and in the development of bio‐inspired glues. The association of these proteins with other components such as ions, proteins, polysaccharides, or polymers enables the fabrication of biomaterials for various healthcare ...
Alessandra Whaite +4 more
wiley +1 more source
Electrochemical control of reversible DNA hybridisation : for future use in nucleic acid amplification [PDF]
, 2014 Denaturation and renaturation is indispensable for the biological function of nucleic acids in many cellular processes, such as for example transcription for the synthesis of RNA and DNA replication during cell division.
Syed, Shahida Nina
core
Processing of DNA Topoisomerase II–DNA–Protein Crosslinks Associated With Anticancer Drugs
Genes to Cells, Volume 31, Issue 1, January 2026.Topoisomerase II (TOP2) relieving DNA torsional stress is trapped by TOP2 poisons, such as etoposide, inducing DNA double‐strand breaks (DSBs) bearing TOP2–DNA–protein crosslinks (TOP2–DPCs), which must be removed before DSB repair. This review summarizes the TOP2–DPC resolution processes mediated by multiple pathways, including proteolysis and ...
Ryo Sakasai, Kuniyoshi Iwabuchi
wiley +1 more source
ChemBioChem, Volume 26, Issue 17, September 15, 2025.When placed in the middle of a double helix as a nucleobase surrogate, pyren‐1‐ylpyridine and its palladacyclic derivative show fluorescence emission sensitive to the identity of the canonical nucleobase opposite to it. Emission of the palladacycle is weaker but the base discrimination stronger, possibly because of Pd(II)‐mediated base pairing.
Dattatraya Uttam Ukale, Tuomas Lönnberg
wiley +1 more source
Profiling RNA G‐Quadruplexes In vivo
Current Protocols, Volume 5, Issue 9, September 2025.Abstract RNA G‐quadruplexes (RG4s) are specific and complex tertiary structures that form in guanine‐rich regions of RNA and can be detected in vitro. A recently developed transcriptome‐wide technique, SHALiPE‐Seq, enables the assessment of RG4 folding status within living cells in a quantitative manner.
Bibo Yang, Yiliang Ding, Yueying Zhang
wiley +1 more source