Results 91 to 100 of about 55,866 (243)

Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein

, 2012
The detection of proteinaceous antigens generally relies on traditional immunoassays and, more recently, on immuno-PCR (polymerase chain reaction) assays and their derivatives, which do not take advantage of the intrinsic function or binding property of ...
Patrick M. Schaeffer, Schaeffer, Patrick M., Morin, Isabelle, Isabelle Morin   +3 more
core   +1 more source

Detection of Rabies’ ribo-nucleoprotein complex using Direct Immunofluorescence Assay.

, 2023
Negative (A) and Positive (B) brain samples were fixed, analysed for the presence of the Rabies virus antigen and visualized under a fluorescence microscope (100X objective). Arrows indicate viral ribo-nucleoprotein complex.
Isabelle Conclois (17271348), Rodrigue Simonet Poueme Namegni (17271336), Laurent God-Yang (17271354), Mohamed Moctar Mouiche Mouliom (17271339), Ranyl Nguena Guefack Noumedem (17271345), Julius Awah-Ndukum (7280063), Simon Dickmu Jumbo (17271342), Jean Marc Kameni Feussom (17328445), Abel Wade (383313), Isaac Dah (17271333), Dorothée Missé (233159), Liegeois Florian (17271351)   +11 more
core   +1 more source

Nucleoprotein Autoantibodies in Lupus Erythematosus

Journal of Investigative Dermatology, 1985
Autoantibodies against deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) proteins are commonly detected in patients with lupus erythematosus (LE). Antibodies against native DNA are frequently detected in a subset of LE patients with a high prevalence of renal disease.
Provost, Thomas T., Herrera-Esparza, Rafael, Diaz, Luis A.   +2 more
openaire   +2 more sources

SOX30 Governs Synaptonemal Complex Assembly and Homologous Recombination in Male Meiosis

Cell Proliferation, Volume 59, Issue 6, June 2026.
The transcription factor SOX30 directly binds promoter regions of SYCE1 and SYCE2 to mediate their transcriptional activation, thereby enabling proper assembly of central elements within the synaptonemal complex. Structural destabilisation of the synaptonemal complex in Sox30 KK spermatocytes triggers synaptic discontinuity, impairs homologous ...
Kangle Liu, Wenfeng Zhang, Xiao Jiang, Jianping Chen, Lei Zhu, Zhonghao Zhang, Jing Gu, Lulu Guo, Lin Ao, Qing Chen, Lei Sun, Yuhan Hu, Xin Wang, Yaxin Liu, Jia Cao, Fei Han, Jinyi Liu   +16 more
wiley   +1 more source

Tyrosine kinase LYN restricts the replication and virulence of influenza A virus by directly phosphorylating viral nucleoprotein

mBio
The continuous antigenic shift and drift of influenza A virus (IAV) result in the emergence of novel strains and drug resistance. Accordingly, it is urgent to develop novel antiviral drugs that target host factors.
Junwen Liu, Jiaxin Huang, Qian Wang, Yanli Wei, Lebin Han, Xin Li, Caoqi Lei, Guohua Deng, Hualan Chen, Qiyun Zhu, Shuai Xu   +10 more
doaj   +1 more source

Phenotypic Consequence of Rearranging the N Gene of RABV HEP-Flury

Viruses, 2019
Nucleoprotein (N) is a key element in rabies virus (RABV) replication. To further investigate the effect of N on RABV, we manipulated an infectious cDNA clone of the RABV HEP-Flury to rearrange the N gene from its wild-type position of 1 (N-P-M-G-L) to 2
Mingzhu Mei, Teng Long, Qiong Zhang, Jing Zhao, Qin Tian, Jiaojiao Peng, Jun Luo, He Jiang, Yingyi Lin, Zhixiong Lin, Xiaofeng Guo   +10 more
doaj   +1 more source

Separation of nucleoprotein complexes with antioxidant activity from yeast Saccharomyces cerevisiae

, 2007
In this study, complexes of nucleic acids and acidic nuclear proteins were isolated from baker's yeast cells of Saccharomyces cerevisiae by mild alkaline extraction followed by precipitation with acetic acid. The optimal composition of the buffer applied
Butylina, Svetlana,, Shataeva, Larisa K.,, Nyström, Marianne,   +2 more
core   +1 more source

Automodification of N‐terminal serine residues facilitates PARP2 release from DNA

The FEBS Journal, Volume 293, Issue 11, Page 3274-3287, June 2026.
PARP2 is involved in detecting DNA damage and its N‐terminal role is largely unknown. Based on biochemical and biophysical data, our findings suggest that in the presence of HPF1, N‐terminal serine 8 and 73 are enriched in auto‐ADP‐ribosylation. Our results provide insight into the mechanistic role of PARP2 N‐terminus in the PARylation‐dependent ...
Saurabh Singh Dhakar, Albert Galera‐Prat, Jin Cai, Julia Preisser, Tiila‐Riikka Kiema, Andreas Ladurner, Lari Lehtiö   +6 more
wiley   +1 more source

The PACS-2 protein and trafficking motifs in CCHFV Gn and Gc cytoplasmic domains govern CCHFV assembly

Emerging Microbes and Infections
The Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne bunyavirus that causes high mortality in humans. This enveloped virus harbors two surface glycoproteins (GP), Gn and Gc, that are released by processing of a glycoprotein precursor complex
Anupriya Gautam, Alexandre Lalande, Maureen Ritter, Natalia Freitas, Solène Lerolle, Lola Canus, Fouzia Amirache, Vincent Lotteau, Vincent Legros, François-Loïc Cosset, Cyrille Mathieu, Bertrand Boson   +11 more
doaj   +1 more source

The aging paradox of Cushing's syndrome: Stress without clear senescence?

Journal of Neuroendocrinology, Volume 38, Issue 6, June 2026.
Abstract Cushing's syndrome (CS), characterized by chronic endogenous hypercortisolism and disruption of the normal circadian cortisol rhythm, represents a unique human model for investigating the interplay between stress endocrinology and biological aging.
Israel Nunes Silveira, Alexandro Guterres   +1 more
wiley   +1 more source