Results 61 to 70 of about 64,059 (299)

Asymmetrically Modified Nucleosomes [PDF]

Cell, 2012
Mononucleosomes, the basic building blocks of chromatin, contain two copies of each core histone. The associated posttranslational modifications regulate essential chromatin-dependent processes, yet whether each histone copy is identically modified in vivo is unclear.
Benjamin A. Garcia, Danny Reinberg, David B. Beck, Barry M. Zee, Nicolas L. Young, William J. Drury, Jinsook Son, Gary LeRoy, Philipp Voigt   +8 more
openaire   +3 more sources

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500‐times more potent and odorless alternative to butyrate

FEBS Open Bio, EarlyView.
Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann   +4 more
wiley   +1 more source

Emerging roles of BET proteins in transcription and co‐transcriptional RNA processing

WIREs RNA, Volume 14, Issue 1, January/February 2023., 2023
Roles of BET proteins in RNA polymerase II transcription and co‐transcriptional RNA processing. Abstract Transcription by RNA polymerase II (Pol II) gives rise to all nuclear protein‐coding and a large set of non‐coding RNAs, and is strictly regulated and coordinated with RNA processing.
Nicole Eischer, Mirjam Arnold, Andreas Mayer   +2 more
wiley   +1 more source

Nucleosome footprinting in plasma cell-free DNA for the pre-surgical diagnosis of ovarian cancer

npj Genomic Medicine, 2022
Fragmentation patterns of plasma cell-free DNA (cfDNA) are known to reflect nucleosome positions of cell types contributing to cfDNA. Based on cfDNA fragmentation patterns, the deviation in nucleosome footprints was quantified between diagnosed ovarian ...
Adriaan Vanderstichele, Pieter Busschaert, Chiara Landolfo, Siel Olbrecht, An Coosemans, Wouter Froyman, Liselore Loverix, Nicole Concin, Elena Ioana Braicu, Pauline Wimberger, Els Van Nieuwenhuysen, Sileny N. Han, Toon Van Gorp, Tom Venken, Ruben Heremans, Patrick Neven, Tom Bourne, Ben Van Calster, Dirk Timmerman, Diether Lambrechts, Ignace Vergote   +20 more
doaj   +1 more source

The dynamics of individual nucleosomes controls the chromatin condensation pathway: direct AFM visualization of variant chromatin [PDF]

, 2009
Chromatin organization and dynamics is studied in this work at scales ranging from single nucleosome to nucleosomal array by using a unique combination of biochemical assays, single molecule imaging technique and numerical modeling. We demonstrate that a subtle modification in the nucleosome structure induced by the histone variant H2A.Bbd drastically ...
arxiv   +1 more source

Long non‐coding RNAs as therapeutic targets in head and neck squamous cell carcinoma and clinical application

FEBS Open Bio, EarlyView.
Long non‐coding RNAs (lncRNAs) occupy an abundant fraction of the eukaryotic transcriptome and an emerging area in cancer research. Regulation by lncRNAs is based on their subcellular localization in HNSCC. This cartoon shows the various functions of lncRNAs in HNSCC discussed in this review.
Ellen T. Tran, Ruchi A. Patel, Amogh Chariyamane, Ratna B. Ray   +3 more
wiley   +1 more source

Large‐scale genetic screens identify BET‐1 as a cytoskeleton regulator promoting actin function and life span

Aging Cell, Volume 22, Issue 1, January 2023., 2023
Using a cross‐species screening approach combining genome‐wide CRISPR‐Cas9 screening in human fibroblasts (1) and synthetic lethality screening in Caenorhabditis elegans (2), we identified BET‐1/BRD4 as a novel regulator of the actin cytoskeleton (3). BET‐1/BRD4 promotes actin filament stability in C.
Gilberto Garcia, Raz Bar‐Ziv, Maxim Averbukh, Nirmalya Dasgupta, Naibedya Dutta, Hanlin Zhang, Wudi Fan, Darius Moaddeli, C. Kimberly Tsui, Toni Castro Torres, Athena Alcala, Erica A. Moehle, Sally Hoang, Ophir Shalem, Peter D. Adams, Max A. Thorwald, Ryo Higuchi‐Sanabria   +16 more
wiley   +1 more source

FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation. [PDF]

PLoS ONE, 2011
Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer.
Jennifer R Stevens, Allyson F O'Donnell, Troy E Perry, Jeremy J R Benjamin, Christine A Barnes, Gerald C Johnston, Richard A Singer   +6 more
doaj   +1 more source

Chromatin Organization Governs Transcriptional Response and Plasticity of Cancer Stem Cells

Advanced Science, EarlyView.
Through a combination of transcriptomic and histone mapping studies integrated with chromatin imaging, we define how chromatin organization in nanoscale packing domains fine tunes the plasticity of cancer stem cells (CSCs) driving evasion from and survival after chemotherapy.
Yinu Wang, Jane Frederick, Karla Isabel Medina, Elizabeth Thomas Bartom, Luay Matthew Almassalha, Yaqi Zhang, Greta Wodarcyk, Hao Huang, I Chae Ye, Ruyi Gong, Cody Levi Dunton, Alex Duval, Paola Carrillo Gonzalez, Joshua Pritchard, John Carinato, Iuliia Topchu, Junzui Li, Zhe Ji, Mazhar Adli, Vadim Backman, Daniela Matei   +20 more
wiley   +1 more source

Tension-dependent Free Energies of Nucleosome Unwrapping [PDF]

, 2016
Nucleosomes form the basic unit of compaction within eukaryotic genomes and their locations represent an important, yet poorly understood, mechanism of genetic regulation. Quantifying the strength of interactions within the nucleosome is a central problem in biophysics and is critical to understanding how nucleosome positions influence gene expression.
arxiv   +1 more source