Results 1 to 10 of about 74,179 (293)
Histone dynamics mediate DNA unwrapping and sliding in nucleosomes
Nature Communications, 2021 Nucleosomes are elementary building blocks of chromatin in eukaryotes. They tightly wrap ∼147 DNA base pairs around an octamer of histone proteins. How nucleosome structural dynamics affect genome functioning is not completely clear.
Grigoriy A Armeev +2 more
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Structural basis for the inhibition of cGAS by nucleosomes
Science, 2020 Saving a host cell from itself A fundamental mammalian defense mechanism against pathogens and damaged cellular DNA is to recognize DNA fragments in the cytosol and trigger an inflammatory response.
Tomoya Kujirai +2 more
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Mechanisms of OCT4-SOX2 motif readout on nucleosomes
Science, 2020 Engaging the nucleosome Cell identity is defined by gene expression patterns that are established through the binding of specific transcription factors.
Alicia K Michael +2 more
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Nucleosomes impede Cas9 access to DNA in vivo and in vitro
eLife, 2016 The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes.
Max A Horlbeck +8 more
doaj +2 more sources
DNA damage detection in nucleosomes involves DNA register shifting
Nature, 2019 Access to DNA packaged in nucleosomes is critical for gene regulation, DNA replication and DNA repair. In humans, the UV-damaged DNA-binding protein (UV-DDB) complex detects UV-light-induced pyrimidine dimers throughout the genome; however, it remains ...
Syota Matsumoto +2 more
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Nucleic Acids Research, 2019 Chromatin organization is crucial for regulating gene expression. Previously, we showed that nucleosomes form groups, termed clutches. Clutch size correlated with the pluripotency grade of mouse embryonic stem cells and human induced pluripotent stem ...
Jason J Otterstrom +2 more
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A core viral protein binds host nucleosomes to sequester immune danger signals
Nature, 2016 Viral proteins mimic host protein structure and function to redirect cellular processes and subvert innate defenses. Small basic proteins compact and regulate both viral and cellular DNA genomes.
Daphne C Avgousti +2 more
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BRD4 is a histone acetyltransferase that evicts nucleosomes from chromatin
Nature Structural and Molecular Biology, 2016 Bromodomain protein 4 (BRD4) is a chromatin-binding protein implicated in cancer and autoimmune diseases that functions as a scaffold for transcription factors at promoters and super-enhancers.
Daoud Meerzaman, Keiko Ozato
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A method for cryo-EM analysis of eukaryotic nucleosomes reconstituted in bacterial cells [PDF]
iScienceSummary: Conventional methods for preparing nucleosomes are time-consuming and technically demanding. In the present study, we extended the approach of generating nucleosomes in Escherichia coli by the co-expression of all four histones, allowing ...
Cheng-Han Ho +4 more
doaj +2 more sources
Structural basis for retroviral integration into nucleosomes
Nature, 2015 Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome. How the intasome interfaces with chromosomal DNA, which exists in the form of nucleosomal arrays, is currently ...
Daniel P Maskell +2 more
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