Results 21 to 30 of about 69,179 (216)

Nucleosome Positioning [PDF]

ISRN Molecular Biology, 2012
Nucleosome positioning is not only related to genomic DNA compaction but also to other biological functions. After the chromatin is digested by micrococcal nuclease, nucleosomal (nucleosome-bound) DNA fragments can be sequenced and mapped on the genomic DNA sequence.
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Nucleosomes in serum as a marker for cell death [PDF]

, 2001
The concentration of nucleosomes is elevated in blood of patients with diseases which are associated with enhanced cell death. In order to detect these circulating nucleosomes, we used the Cell Death Detection-ELISA(Plus) (CDDE) from Roche Diagnostics ...
Anker P, Arends MJ, Burlingame RW, Du Clos TW, Emlen W, Gauthier VJ, Holdenrieder S, Jahr S, Leist M, Leon SA, Maebo A, Majno G, Mannherz HG, Rumore P, Stroun M, Watson RW   +15 more
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Nucleosomes in pancreatic cancer patients during radiochemotherapy [PDF]

, 2005
Nucleosomes appear spontaneously in elevated concentrations in the serum of patients with malignant diseases as well as during chemo- and radiotherapy. We analyzed whether their kinetics show typical characteristics during radiochemotherapy and enable an
Amoura Z, Andreas Kremer, Arends MJ, Dietrich Seidel, Dorothea Nagel, Gauthier VJ, Heinemann V, Holdenrieder S, Ishii H, Kerr JF, Kuroi K, Lo YM, Meyn RE, Petra Stieber, Ralf Wilkowski, Rumore P, Stefan Holdenrieder, Szumiel I, Wilkowski R, Wyllie AH   +19 more
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High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier. [PDF]

, 2019
Nucleosomes represent mechanical and energetic barriers that RNA Polymerase II (Pol II) must overcome during transcription. A high-resolution description of the barrier topography, its modulation by epigenetic modifications, and their effects on Pol II ...
Brown, Aidan I, Bustamante, Carlos, Chen, Zhijie, Díaz-Celis, César, Gabizon, Ronen, Kaplan, Craig D, Koslover, Elena F, Lee, Antony, Song, Aixin, Yao, Tingting   +9 more
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Nucleosomes and neutrophil extracellular traps in septic and burn patients [PDF]

, 2017
NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1
Carestia, Agostina, D'Atri, Lina Paola, Fandiño, María Eugenia, Fondevila, Carlos, Guzman, María Alejandra, Kaufman, Tomás, Magosevich, Débora, Moreno, María Carolina, Schattner, Mirta Ana   +8 more
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Structural characterization of H3K56Q nucleosomes and nucleosomal arrays [PDF]

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010
The post-translational modification of histones is a key mechanism for the modulation of DNA accessibility. Acetylated lysine 56 in histone H3 is associated with nucleosome assembly during replication and DNA repair, and is thus likely to predominate in regions of chromatin containing nucleosome-free regions.
Karolin Luger, Karolin Luger, Michael G. Resch, Nicholas J. Clark, Shinya Watanabe, Wayne Lilyestrom, Jeffrey C. Hansen, Craig L. Peterson   +7 more
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DNA folding: structural and mechanical properties of the two-angle model for chromatin [PDF]

, 2001
We present a theoretical analysis of the structural and mechanical properties of the 30-nm chromatin fiber. Our study is based on the two-angle model introduced by Woodcock et al. (Woodcock, C. L., S. A. Grigoryev, R. A. Horowitz, and N. Whitaker.
Bednar, Bednar, Butler, Cluzel, Crothers, Cui, de Gennes, Doi, Finch, Fraden, Hagerman, Helmut Schiessel, Horowitz, Katritch, Khrapunov, Klenin, Leuba, Livolant, Luger, Marko, Marko, Nyrkova, Plewa, Raspaud, Robijn Bruinsma, Schlick, Smith, Stryer, Thoma, van Holde, van Holde, van Holde, Widom, Widom, Widom, Widom, Widom, William M. Gelbart, Woodcock, Yao   +39 more
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Nucleosome packaging and nucleosome positioning of genomic DNA [PDF]

Proceedings of the National Academy of Sciences, 1997
The goals of this study were to assess the extent to which bulk genomic DNA sequences contribute to their own packaging in nucleosomes and to reveal the relationship between nucleosome packaging and positioning. Using a competitive nucleosome reconstitution assay, we found that at least 95% of bulk DNA sequences have an affinity for ...
J. Widom, P. T. Lowary
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The Snf2 Homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme [PDF]

, 2010
The Saccharomyces cerevisiae Fun30 (Function unknown now 30) protein shares homology with an extended family of Snf2-related ATPases. Here we report the purification of Fun30 principally as a homodimer with a molecular mass of about 250 kDa.
Adra, Ahmed H. Hassan, Barton, Becker, Bruno, Cairns, Clark, Collins, Daniel Ryan, Dillingham, Ferreira, Ferreira, Flaus, Flaus, Gavin, Hartley, Hassan, Hassan, Kouzarides, Krogan, Lowary, Luger, Mizuguchi, Neves-Costa, Okazaki, Ouspenski, Papamichos-Chronakis, Philippe Prochasson, Puig, Racki, Salma Awad, Schoor, Schoor, Shih, Smith, Soininen, Stockdale, Strohner, Suter, Thåström, Tom Owen-Hughes, Ubersax, Whitehouse, Zofall   +43 more
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Nucleosomes and Cisplatin

Chemistry & Biology, 2010
In order to form a covalent complex with DNA inside human cells, cisplatin has to overcome the protective environment of a nucleosome, where DNA is complexed with histone proteins. Todd and Lippard (2010) expand our understanding of this process by describing the structure of a nucleosome containing a Pt-DNA adduct, which has important implications for
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