Results 21 to 30 of about 71,049 (222)
Biomolecules, 2022 Background: Neutrophil extracellular traps’ (NETs’) formation is a mechanism of defense that neutrophils deploy as an alternative to phagocytosis, to constrain the spread of microorganisms.
Laure Morimont +14 more
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Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes
Nature Structural & Molecular Biology, 2018 Epigenetic regulation is mediated by protein complexes that couple recognition of chromatin marks to activity or recruitment of chromatin-modifying enzymes. Polycomb repressive complex 2 (PRC2), a gene silencer that methylates lysine 27 of histone H3, is
S. Poepsel, V. Kasinath, E. Nogales
semanticscholar +1 more source
Cell Reports, 2021 Summary: The ARID1A subunit of SWI/SNF chromatin remodeling complexes is a potent tumor suppressor. Here, a degron is applied to detect rapid loss of chromatin accessibility at thousands of loci where ARID1A acts to generate accessible minidomains of ...
Seraina Blümli +13 more
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Mechanical properties of nucleoprotein complexes determined by nanoindentation spectroscopy
Nucleus, 2020 The interplay between transcription factors, chromatin remodelers, 3-D organization, and mechanical properties of the chromatin fiber controls genome function in eukaryotes.
Tatini Rakshit +3 more
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Quantitative test of the barrier nucleosome model for statistical positioning of nucleosomes up- and downstream of transcription start sites [PDF]
PLoS Comp. Biol. 6, e1000891 (2010), 2010 The positions of nucleosomes in eukaryotic genomes determine which parts of the DNA sequence are readily accessible for regulatory proteins and which are not. Genome-wide maps of nucleosome positions have revealed a salient pattern around transcription start sites, involving a nucleosome-free region (NFR) flanked by a pronounced periodic pattern in the
arxiv +1 more source
Dissociation rate compensation mechanism for budding yeast pioneer transcription factors
eLife, 2019 Nucleosomes restrict the occupancy of most transcription factors (TF) by reducing binding and accelerating dissociation, while a small group of TFs have high affinities to nucleosome-embedded sites and facilitate nucleosome displacement.
Benjamin T Donovan +4 more
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Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
Proceedings of the National Academy of Sciences of the United States of America, 2018 Significance The efficiency of genome editing with CRISPR-Cas9 can vary widely at different targets and in different cells. Some of this variability may be due to the inherent quality of different guide RNAs, but it may also depend on the cellular ...
R. Yarrington +4 more
semanticscholar +1 more source
BRD4 is a Histone Acetyltransferase that Evicts Nucleosomes from Chromatin
Nature Structural &Molecular Biology, 2016 Bromodomain protein 4 (BRD4) is a chromatin-binding protein implicated in cancer and autoimmune diseases that functions as a scaffold for transcription factors at promoters and super-enhancers.
B. N. Devaiah +8 more
semanticscholar +1 more source
Precise genome-wide mapping of single nucleosomes and linkers in vivo
Genome Biology, 2018 We developed a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high accuracy genome-wide in yeast.
Răzvan V. Chereji +3 more
semanticscholar +1 more source
Epigenetics, 2018 Previous work in Saccharomyces cerevisiae identified three residues located in close proximity to each other on the side of the nucleosome whose integrity is required for proper association of the Spt16 component of the FACT complex across transcribed ...
Eugene Nyamugenda +8 more
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