Results 31 to 40 of about 74,179 (293)
The Role of Histone Variants in the Epithelial-To-Mesenchymal Transition
Cells, 2020 The epithelial-to-mesenchymal transition (EMT) is a physiological process activated during early embryogenesis, which continues to shape tissues and organs later on. It is also hijacked by tumor cells during metastasis. The regulation of EMT has been the
Imtiaz Nisar Lone +4 more
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The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome. [PDF]
PLoS Genetics, 2008 Chromatin structure plays an important role in modulating the accessibility of genomic DNA to regulatory proteins in eukaryotic cells. We performed an integrative analysis on dozens of recent datasets generated by deep-sequencing and high-density tiling ...
Yutao Fu +3 more
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Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes
Nature Structural & Molecular Biology, 2018 Epigenetic regulation is mediated by protein complexes that couple recognition of chromatin marks to activity or recruitment of chromatin-modifying enzymes. Polycomb repressive complex 2 (PRC2), a gene silencer that methylates lysine 27 of histone H3, is
S. Poepsel, V. Kasinath, E. Nogales
semanticscholar +1 more source
Dissociation rate compensation mechanism for budding yeast pioneer transcription factors
eLife, 2019 Nucleosomes restrict the occupancy of most transcription factors (TF) by reducing binding and accelerating dissociation, while a small group of TFs have high affinities to nucleosome-embedded sites and facilitate nucleosome displacement.
Benjamin T Donovan +4 more
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Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes
eLife, 2020 SWI/SNF-family chromatin remodeling complexes, such as S. cerevisiae RSC, slide and eject nucleosomes to regulate transcription. Within nucleosomes, stiff DNA sequences confer spontaneous partial unwrapping, prompting whether and how SWI/SNF-family ...
Alisha Schlichter +3 more
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Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
Proceedings of the National Academy of Sciences of the United States of America, 2018 Significance The efficiency of genome editing with CRISPR-Cas9 can vary widely at different targets and in different cells. Some of this variability may be due to the inherent quality of different guide RNAs, but it may also depend on the cellular ...
R. Yarrington +4 more
semanticscholar +1 more source
Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation
eLife, 2021 In meiosis, DNA double-strand break (DSB) formation by Spo11 initiates recombination and enables chromosome segregation. Numerous factors are required for Spo11 activity, and couple the DSB machinery to the development of a meiosis-specific ‘axis ...
Dorota Rousová +10 more
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Precise genome-wide mapping of single nucleosomes and linkers in vivo
Genome Biology, 2018 We developed a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high accuracy genome-wide in yeast.
Răzvan V. Chereji +3 more
semanticscholar +1 more source
Epichromatin and chromomeres: a ‘fuzzy’ perspective [PDF]
Open Biology, 2018 ‘Epichromatin’, the surface of chromatin beneath the interphase nuclear envelope (NE) or at the surface of mitotic chromosomes, was discovered by immunostaining with a specific bivalent mouse monoclonal anti-nucleosome antibody (mAb PL2-6). ‘Chromomeres’,
Donald E. Olins, Ada L. Olins
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Biomolecules, 2022 Background: Neutrophil extracellular traps’ (NETs’) formation is a mechanism of defense that neutrophils deploy as an alternative to phagocytosis, to constrain the spread of microorganisms.
Laure Morimont +14 more
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