Results 101 to 110 of about 12,120 (223)

Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration [PDF]

, 2015
INTRODUCTION: Early degenerative changes in the nucleus pulposus (NP) are observed after the disappearance of notochordal cells (NCs). Thus, it has been suggested that NCs play an important role in maintaining the NP and may have a regenerative potential
Björn P Meij, Smolders, Lucas A, Potier, Esther, Lucas A Smolders, LS Algemene chirurgie, Arkesteijn, ITM Irene, Potier, E Esther, Spillekom, S, Spillekom, S., Tryfonidou, M.A., Meij, Björn P, Riemers, Frank M, Riemers, F.M., Spillekom, Sandra, Arkesteijn, I.T.M., Sub Neuro/Tandheelkunde, Irene TM Arkesteijn, Marianna A Tryfonidou, Ito, K Keita, Sandra Spillekom, Esther Potier, Keita Ito, Meij, B.P., Frank M Riemers, CSCA TR1, Smolders, LA, Sub Neuro Chirurgie, Smolders, L.A., Biochemisch laboratorium, Ito, K., Ito, K, Potier, E., Arkesteijn, Irene Tm, Ito, Keita, Tryfonidou, Marianna A, Regenerative Medicine, Stem Cells & Cancer, Meij, BP, Tryfonidou, MA   +37 more
core   +1 more source

Minimal Sustainability of Dedifferentiation by ROCK Inhibitor on Rat Nucleus Pulposus Cells In Vitro

Spine Surgery and Related Research, 2019
Introduction: Intervertebral disc degeneration is strongly associated with low back pain. Cell transplantation has been extensively studied as a treatment option for intervertebral disc degeneration. It is often necessary to perform cell culture prior to
Tadashi Nukaga, Daisuke Sakai, Jordy Schol, Kaori Suyama, Tomoko Nakai, Akihiko Hiyama, Masahiko Watanabe   +6 more
doaj   +1 more source

TIE2-positive cells in the nucleus pulposus with a purpose: the who, what and why

Journal of Biomedical Science
Abstract Once thought to be solely involved in vasculogenesis, tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (TIE2) has emerged as a crucial marker of progenitor-like cells in the avascular nucleus pulposus (NP), a tissue with notoriously limited regenerative capacity.
Schol, Jordy, Ambrosio, Luca, Ruiz-Fernandez, Clara, Schlagenhof, Leon, Voskamp, Chantal, Laagland, Lisanne T, Matsushita, Erika, Soma, Hazuki, Warita, Takayuki, Vadalà, Gianluca, Tryfonidou, Marianna A, Gantenbein, Benjamin, Sakai, Daisuke   +12 more
openaire   +2 more sources

Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cells

, 2011
There is evidence that mesenchymal stem cells (MSCs) can differentiate towards an intervertebral disc (IVD)-like phenotype. We compared the standard chondrogenic protocol using transforming growth factor beta-1 (TGFß) to the effects of hypoxia, growth ...
Aebli, N, M Alini, M Baur, N Aebli, Bertolo, A, Grad, S, B Gantenbein-Ritter, Gantenbein-Ritter, Benjamin, Alini, M, JV Stoyanov, A Bertolo, Stoyanov, JV, Baur, M, S Grad   +13 more
core  

Effect of electroacupuncture on expression of autophagy-related proteins in nucleus pulposus of cervical spondylosis rabbits

针刺研究
ObjectiveTo observe the effects of electroacupuncture （EA） on the morphological changes of intervertebral disc tissues， apoptosis of nucleus pulposus cells， and the protein expression of Unc-51 like autophagy-activated kinase 1 （ULK1）， homologous series ...
CHEN Jia-li, ZHAO Zi-ying, GAO Hang, XU Yin-qing, WANG Guang-yi   +4 more
doaj  

Fibro-NPC: a pathogenic subtype identified at single-cell resolution with secreted SFRP4 as a biomarker in intervertebral disc degeneration

Journal of Translational Medicine
Background Intervertebral disc degeneration (IDD) is a primary cause of low back pain and, in severe cases, can lead to disability. Current treatments for low back pain remain limited in efficacy, underscoring the need for a deeper understanding of the ...
Yili Xu, Zuozhi Xie, Shubo Gu, Xiang Zhao, Di Zhao, Zhengzheng Wu, Jinfeng Wang, Tao Xu, Ruxue Xu, Zhenhua Feng, Yong Qiu, Zezhang Zhu, Yang Yu, Bo Shi, Jun Jiang   +14 more
doaj   +1 more source

A step‐by‐step protocol for isolation of murine nucleus pulposus cells

JOR SPINE, 2019
AbstractThe intervertebral disc (IVD) is composed of three separate tissues with distinct origins and properties. Elucidating changes occurring in these tissues in response to injury or age is paramount to identify new therapies to better manage disc and spine degenerative conditions, including low back pain.
Andrew Bratsman, Greig Couasnay, Florent Elefteriou   +2 more
openaire   +3 more sources

Autoimmune properties of nucleus pulposus: an experimental study in pigs

, 2007
STUDY DESIGN: Assessment of activated T and B cells in a subcutaneous chamber filled with autologous nucleus pulposus using flow cytometry and immunohistochemistry.
Rydevik, Björn,, Olmarker, Kjell,, Larsson, Karin,, Takahashi, I., Geiss, A.   +4 more
core  

Co-culture of Notochordal Cells with Nucleus pulposus and Annulus fibrosus cells under normoxia and hypoxia

, 2014
Introduction Notochordal cells (NC) are shifted back into focus due to their apparent action of activating other disc cells via indirect release of yet unknown factors into the medium (conditioned medium = CM).1,2 Recent evidence confirms the results ...
Calandriello, Elena, Keel, Marius, Gantenbein, Benjamin, Chan, Samantha, Benneker, Lorin Michael, Wuertz, K.   +5 more
core   +1 more source

The long non-coding RNA NEAT1 contributes to extracellular matrix degradation in degenerative human nucleus pulposus cells

, 2018
Intervertebral disc degeneration is a complex disease involving genetic and environmental factors and multiple cellular processes. The role and expression of the lncRNA NEAT1 were assessed in intervertebral disc degeneration.
Jing Li, Hui Ma, Huiyong Liu, Haoruo Jia, Zhi Ruan, Feng Li   +5 more
core   +1 more source