Results 111 to 120 of about 4,980,963 (235)

Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

, 2021
Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from ...
Surtel, Donatus A M, Voncken, Willem, Cremers, Andy, van den Akker, Guus G H, Welting, Tim J M   +4 more
core   +1 more source

A step‐by‐step protocol for isolation of murine nucleus pulposus cells

JOR SPINE, 2019
AbstractThe intervertebral disc (IVD) is composed of three separate tissues with distinct origins and properties. Elucidating changes occurring in these tissues in response to injury or age is paramount to identify new therapies to better manage disc and spine degenerative conditions, including low back pain.
Andrew Bratsman, Greig Couasnay, Florent Elefteriou   +2 more
openaire   +3 more sources

Characterization of the age-dependent intervertebral disc changes in rabbit by correlation between MRI, histology and gene expression

BMC Musculoskeletal Disorders, 2011
Background The present study was conducted to address whether the intervertebral disc of rabbit could be considered (i) as a valuable model to provide new insights into the tissue and cellular changes of Nucleus pulposus aging and (ii) as an appropriate ...
Gauthier Olivier, Fellah Borhane H, Lesoeur Julie, Masson Martial, Grimandi Gaël, Pot-Vaucel Marianne, Clouet Johann, Fusellier Marion, Cherel Yan, Maugars Yves, Guicheux Jérôme, Vinatier Claire   +11 more
doaj   +1 more source

Understanding extracellular vesicles in antiaging

Periodontology 2000, EarlyView.
Abstract Background Aging is a multifactorial process marked by gradual cellular dysregulation, regulated by hallmarks, including genomic instability, mitochondrial dysregulation, and chronic inflammation. Extracellular vesicles (EVs), especially exosomes, have emerged as pivotal mediators of cell‐to‐cell communication with potential roles in both ...
Paras Ahmad, Nathan E. Estrin, Anton Sculean, Richard J. Miron   +3 more
wiley   +1 more source

The role of fenestration in acute canine thoracolumbar intervertebral disc extrusion: Part 2—The biological and biomechanical effects of fenestration

Veterinary Surgery, EarlyView.
Abstract Background Prophylactic fenestration is widely performed to prevent recurrence of canine acute thoracolumbar intervertebral disc extrusion (IVDE); however, there is a paucity of specific data regarding the biological and biomechanical effect of this procedure on the intervertebral disc and vertebral column.
James D. Crowley, Paul Freeman, William R. Walsh   +2 more
wiley   +1 more source

Canonical and non‐canonical functions of proteins regulating mitochondrial dynamics in mammalian physiology

The Journal of Physiology, EarlyView.
Abstract figure legend Mitochondria are highly dynamic organelles that continuously remodel their architecture through coordinated cycles of fusion and fission. This review examines the four key GTPases that orchestrate mitochondrial dynamics in mammals: MFN1, MFN2, OPA1, and DRP1.
Rémi Chaney, Vincent Marcangeli, Krystel Desjardins, Shima Taherkhani, Gilles Gouspillou   +4 more
wiley   +1 more source

Hybrid PVA-xanthan gum hydrogels as nucleus pulposus substitutes

, 2019
Hybrid hydrogels were synthesized mixing poly(vinyl alcohol) (PVA) and xanthan gum (XG) in different molar ratios and using trisodium trimetaphosphate, as crosslinking agent, to obtain potential nucleus pulposus substitutes.
Leone, Gemma, Bonechi, Claudia, Consumi, Marco, Lamponi, Stefania, Donati, Alessandro, Magnani, Agnese, Rossi, Claudio, Tamasi, Gabriella   +7 more
core   +1 more source

Biomechanical, Structural, and Composition Characterization of the Vertebra‐Disc Interface

JOR SPINE, Volume 9, Issue 4, December 2026.
ABSTRACT Background The spinal endplate region consists of the cartilage endplate (CEP), bony endplate (BEP), and subchondral trabecular bone (STB). Although this region is often involved in vertebral fractures and disrupted with disc degeneration, the biomechanics of these tissues are incompletely understood. The goal of this study was to characterize
Neilesh R. Frings, Harrah R. Newman, Faysal M. Haque, Veronica A. Caruso, Dawn M. Elliott, Elise F. Morgan   +5 more
wiley   +1 more source

TIE2-positive cells in the nucleus pulposus with a purpose: the who, what and why

Journal of Biomedical Science
Abstract Once thought to be solely involved in vasculogenesis, tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (TIE2) has emerged as a crucial marker of progenitor-like cells in the avascular nucleus pulposus (NP), a tissue with notoriously limited regenerative capacity.
Schol, Jordy, Ambrosio, Luca, Ruiz-Fernandez, Clara, Schlagenhof, Leon, Voskamp, Chantal, Laagland, Lisanne T, Matsushita, Erika, Soma, Hazuki, Warita, Takayuki, Vadalà, Gianluca, Tryfonidou, Marianna A, Gantenbein, Benjamin, Sakai, Daisuke   +12 more
openaire   +3 more sources

Fibro-NPC: a pathogenic subtype identified at single-cell resolution with secreted SFRP4 as a biomarker in intervertebral disc degeneration

Journal of Translational Medicine
Background Intervertebral disc degeneration (IDD) is a primary cause of low back pain and, in severe cases, can lead to disability. Current treatments for low back pain remain limited in efficacy, underscoring the need for a deeper understanding of the ...
Yili Xu, Zuozhi Xie, Shubo Gu, Xiang Zhao, Di Zhao, Zhengzheng Wu, Jinfeng Wang, Tao Xu, Ruxue Xu, Zhenhua Feng, Yong Qiu, Zezhang Zhu, Yang Yu, Bo Shi, Jun Jiang   +14 more
doaj   +1 more source