Results 51 to 60 of about 12,120 (223)

BMSCs-derived exosomes overexpressing TGM2 inhibit lactate-induced oxidative stress related apoptosis in nucleus pulposus cells

陆军军医大学学报
Objective To explore the role and mechanism of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) overexpressing transglutaminase 2 (TGM2) in lactate-mediated oxidative stress-induced apoptosis in nucleus pulposus cells.
LOU Jinhui, GAO Xiaoxin, ZHANG Yuyao
doaj   +1 more source

Transforming growth factor β controls CCN3 expression in nucleus pulposus cells of the intervertebral disc [PDF]

, 2011
Objective To investigate transforming growth factor β (TGFβ) regulation of CCN3 expression in cells of the nucleus pulposus. Methods Real‐time reverse transcription–polymerase chain reaction and Western blot analyses were used to measure CCN3 ...
Rittié, Laure, Symes, Aviva, Irving M. Shapiro, Risbud, Makarand V., Makarand V. Risbud, Cassie M. Tran, Bernard Perbal, Shapiro, Irving M., Smith, Harvey E., Harvey E. Smith, Perbal, Bernard, Laure Rittié, Tran, Cassie M., Aviva Symes   +13 more
core   +1 more source

Cell Adhesion by Design: Engineering Tissue Culture Scaffolds With Adhesion Cues

Advanced Materials Interfaces, EarlyView.
ABSTRACT In scaffold‐based tissue engineering, the matrix should provide adequate adhesion cues for cell attachment, spreading, and function. Given the multitude of adhesion receptors and the diversity of scaffolds, there are many approaches to render scaffolds adhesive, even though they are not all equivalent.
Dalia Dranseike, Dhananjay V. Deshmukh, Mark W. Tibbitt, Céline Labouesse   +3 more
wiley   +1 more source

Piezo2 Mediates a Vicious Cycle of “Mechanical Homeostasis Imbalance—Inflammation” in Sensory Nerves and the Cartilage Endplate

Advanced Science, EarlyView.
In a lumbar spine instability model, dorsal root ganglion cells mediate the perception of relevant mechanical stresses through Piezo2 and subsequently release CGRP. CGRP activates the NF‐κB signaling pathway in cartilage endplate cells through the receptor RAMP1.
Hanpeng Xu, Wen Geng, Zhi Du, Yifan Du, Di Wu, Bide Tong, Huaizhen Liang, Xingyu Zhou, Zixuan Ou, Junyu Wei, Kun Wang, Yu Song, Wenbin Hua, Yan Xu, Wencan Ke, Bingjin Wang, Cao Yang   +16 more
wiley   +1 more source

Alterations in gene expression in response to compression of nucleus pulposus cells [PDF]

The Spine Journal, 2011
It is clear that mechanical forces are involved in initiating disc degeneration but also have the potential to exert beneficial effects. However, the signaling pathways initiated by mechanical stress and thresholds for these responses have not been elucidated. We have developed a metabolically active compression system with the advantages of having the
Gwendolyn A, Sowa, J Paulo, Coelho, Kevin M, Bell, Andrew S, Zorn, Nam V, Vo, Patrick, Smolinski, Christian, Niyonkuru, Robert, Hartman, Rebecca K, Studer, James D, Kang   +9 more
openaire   +2 more sources

Senolytic Therapy as a Preventive Strategy for Spine Degeneration and Pain

Advanced Science, EarlyView.
Cellular senescence promotes inflammation, tissue degeneration, and chronic back pain. In sparc‐null mice, early oral administration of the senolytic agents o‐vanillin and RG‐7112 reduced senescent cell burden and pro‐inflammatory SASP signaling across intervertebral discs, endplates, vertebral bone, and spinal cord.
Saber Ghazizadeh, Hosni Cherif, Matthew Mannarino, Juiena Sagir, Magali Millecamps, Jean A. Ouellet, Laura S. Stone, Lisbet Haglund   +7 more
wiley   +1 more source

In Situ Stress‐Dispersing Hydrogel Millispheres via Load Redistribution to Restore Nucleus Pulposus Metabolic Homeostasis

Advanced Science, EarlyView.
Local stress concentration disrupts metabolic homeostasis and induces inflammation in the nucleus pulposus (NP), thereby accelerating intervertebral disc degeneration (IDD). A biomimetic HA/ChS hydrogel millimeter sphere (ChS@HM) is developed to enable synergistic stress dispersion and sustained hydration lubrication.
Ang Li, Hui Yuan, Honglei Xiao, Xiaodong Liu, Wenguo Cui   +4 more
wiley   +1 more source

Systematic analysis of lysine lactylation in nucleus pulposus cells

iScience
Nucleus pulposus (NP) resides in hypoxic microenvironment and NP cells (NPCs), primarily reply on glycolysis and producing high levels of lactate. Intracellular lactate drives lysine lactylation (Kla) as a newly epigenetic modification. However, the impact of Kla on NPCs remains unknown.
Lei Sheng, Haoran Xu, Yuexing Wang, Jinhao Ni, Taiyang Xiang, Huanhuan Xu, Xiaozhong Zhou, Kang Wei, Jun Dai   +8 more
openaire   +3 more sources

Regulation of human nucleus pulposus cells by peptide-coupled substrates [PDF]

Acta Biomaterialia, 2017
Nucleus pulposus (NP) cells are derived from the notochord and differ from neighboring cells of the intervertebral disc in phenotypic marker expression and morphology. Adult human NP cells lose this phenotype and morphology with age in a pattern that contributes to progressive disc degeneration and pathology.
Devin T, Bridgen, Bailey V, Fearing, Liufang, Jing, Johannah, Sanchez-Adams, Megan C, Cohan, Farshid, Guilak, Jun, Chen, Lori A, Setton   +7 more
openaire   +2 more sources

Leptin Activates RhoA/ROCK Pathway to Induce Cytoskeleton Remodeling in Nucleus Pulposus Cells

, 2014
Hyperleptinemia is implicated in obesity-associated lumbar disc degeneration. Nevertheless, the effect of leptin on the intracellular signaling of nucleus pulposus cells is not clear. The current study sought to delineate the possible involvement of the
Jun Yu, Xisheng Weng, Zheng Li, William Wu, Jinqian Liang, Jianxiong Shen, Xin Yu   +6 more
core   +1 more source