Results 81 to 90 of about 97,181 (355)

Isolation and Culture of Oligodendrocytes

, 2019
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons.
Weil, Marie-Theres, Schulz-Ëberlin, Giselheid, Mukherjee, Chaitali, Kuo-Elsner, Wen Ping, Schäfer, Isabelle, Müller, Christina, Simons, Mikael, Lyons, David A., Kegel, Linde   +8 more
openaire   +3 more sources

Metabolic aspects of Neuronal – Oligodendrocytic - Astrocytic (NOA) interactions

Frontiers in Endocrinology, 2013
Whereas astrocytes have been in the limelight on the metabolic glucose interaction scene for a while, oligodendrocytes are still waiting for a place.
Ana I Amaral, Ana I Amaral, Tore Wergeland Meisingset, Mark R Kotter, Mark R Kotter, Ursula eSonnewald   +5 more
doaj   +1 more source

Signalling through AMPA receptors on oligodendrocyte precursors promotes myelination by enhancing oligodendrocyte survival

eLife, 2017
Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development is unclear. Murine OPs in
E. Kougioumtzidou, Takahiro Shimizu, N. Hamilton, K. Tohyama, R. Sprengel, H. Monyer, D. Attwell, W. Richardson   +7 more
semanticscholar   +1 more source

Investigating the Cellular Effects of GALC Dosing in Enzyme Replacement Therapy for Krabbe Disease Supports the Role of Nanomedicine

Advanced Biology, EarlyView.
A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso, Sara Carpi, Laura Colagiorgio, Miriam De Sarlo, Mariacristina Gagliardi, Marco Cecchini   +5 more
wiley   +1 more source

Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells

Stem Cells and Cloning: Advances and Applications, 2010
Yi-Jang Lee1, Shih-Chieh Hung2–5, Mien-Sheng Chu41Department of Biomedical Imaging and Radiological Sciences, 2Institute of Clinical Medicine, 3Institute of Pharmacology, Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; 4Stem
Yi-Jang Lee, Shih-Chieh Hung, Mien-Sheng Chu   +2 more
doaj  

How Do Cells of the Oligodendrocyte Lineage Affect Neuronal Circuits to Influence Motor Function, Memory and Mood?

Frontiers in Cellular Neuroscience, 2018
Oligodendrocyte progenitor cells (OPCs) are immature cells in the central nervous system (CNS) that can rapidly respond to changes within their environment by modulating their proliferation, motility and differentiation.
Renee E. Pepper, Kimberley A. Pitman, C. L. Cullen, K. Young   +3 more
semanticscholar   +1 more source

Infrared Neural Stimulation Elicits Distinct Molecular Pathways in Astrocytes Based on Laser Pulse Parameters

Advanced Biology, EarlyView.
Infrared (IR) light evokes distinct calcium and water transport responses in astrocytes, depending on stimulation duration and protocol. This study uses widefield imaging and pharmacology to reveal differential engagement of astroglial signaling pathways.
Wilson R. Adams, Ana I Borrachero‐Conejo, Pratheepa Kumari Rasiah, Emanuella Saracino, Roberto Zamboni, Eric Duco Jansen, Valentina Benfenati, Anita Mahadevan‐Jansen   +7 more
wiley   +1 more source

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models

Stem Cell Reports, 2015
In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson’s disease (PD).
Mehdi Djelloul, Staffan Holmqvist, Antonio Boza-Serrano, Carla Azevedo, Maggie S. Yeung, Stefano Goldwurm, Jonas Frisén, Tomas Deierborg, Laurent Roybon   +8 more
doaj   +1 more source

Machine Learning Guided Design of Nerve‐On‐A‐Chip Platforms with Promoted Neurite Outgrowth

Advanced Functional Materials, EarlyView.
Compared to labor‐intensive trial‐and‐error experimentation, a machine learning (ML)‐guided workflow, incorporating cell viability assays, data augmentation, ensemble modeling, and model interpretation, is developed to accelerate nerve‐on‐a‐chip optimization and uncover data‐driven design principles.
Tsai‐Chun Chung, Chu‐Chun Liang, Min‐Wei Huang, Tianle Chen, Joshua M. Little, Snehi Shrestha, Yi‐Chen Ethan Li, I‐Chi Lee, Po‐Yen Chen   +8 more
wiley   +1 more source

Engineering Topographical Cues to Enhance Neural Regeneration in Spinal Cord Injury: Overcoming Challenges and Advancing Therapies

Advanced Functional Materials, EarlyView.
Spinal cord injury (SCI) poses significant challenges for regeneration due to a series of secondary injury mechanisms. How to use biomaterial approach to target the failed regeneration after SCI remains a critical challenge. This review systematically evaluates current strategies to optimize biomaterial topographies for neurite outgrowth, axonal ...
Wei Xu, Fenghui Wang, Joshua Stein, Siqiao Wang, Pengfei Jiang, Letao Yang, Liming Cheng, Ki‐Bum Lee   +7 more
wiley   +1 more source