Results 91 to 100 of about 4,788 (197)
Журнал микробиологии, эпидемиологии и иммунобиологииRelevance. Studies on model systems have confirmed the effectiveness of antisense oligonucleotides, including those that contain photoactive groups, for the modification of nucleic acids.
Asya S. Levina +5 more
doaj +1 more source
A microfluidic oligonucleotide synthesizer [PDF]
, 2010 De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods.
Lee, Cheng-Chung +2 more
core +4 more sources
Reactive oxygen species are signaling molecules that modulate plant reproduction
Plant, Cell &Environment, Volume 47, Issue 5, Page 1592-1605, May 2024.Abstract Reactive oxygen species (ROS) can serve as signaling molecules that are essential for plant growth and development but abiotic stress can lead to ROS increases to supraoptimal levels resulting in cellular damage. To ensure efficient ROS signaling, cells have machinery to locally synthesize ROS to initiate cellular responses and to scavenge ROS
Mohammad Foteh Ali, Gloria K. Muday
wiley +1 more source
Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling [PDF]
, 2012 The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps.
Biertuempfel, Christian +6 more
core +1 more source
Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides
Nucleic Acids Research, 1988 The Rp- and Sp-diastereomers of the phosphorothioate-containing oligonucleotide d[ApAp(S)ApA] have been synthesized. They and the tetramer d[ApApApA] were tested as substrates for staphylococcal nuclease, DNase II and spleen phosphodiesterase. For digestions with DNase I these oligonucleotides were converted to the 5'-phosphorylated derivates.
S, Spitzer, F, Eckstein
openaire +3 more sources
Stimulus‐Responsive Hydrogels as Drug Delivery Systems for Inflammation Targeted Therapy
Advanced Science, Volume 11, Issue 1, January 5, 2024.Deregulated Inflammation is one of the most common diseases in human health, and its management remains a significant challenge. Notably, stimulus‐responsive hydrogels have great potential to break through the predicament faced by severe inflammations treatment.
Haoyu Yu +5 more
wiley +1 more source
Next Generation Gene Synthesis by targeted retrieval of bead-immobilized, sequence verified DNA clones from a high throughput pyrosequencing device [PDF]
, 2013 The setup of synthetic biological systems involving millions of bases is still limited by the required high quality of synthetic DNA. Important drivers to further open up the field are the accuracy and scale of chemical DNA synthesis and the downstream ...
Babrzadeh, Farbod +11 more
core +1 more source
Plant ribosome shunting in vitro [PDF]
, 2017 It has been proposed that cauliflower mosaic virus 35S RNA with its 600 nt long leader uses an unusual translation process (the translational shunt). A wheat germ in vitro translation assay was used to improve the study of this mechanism.
Dominguez, Diana +3 more
core
Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides
Nucleic Acids Research, 1985 Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.
Tomas Kempe +3 more
openaire +3 more sources
HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N6-methyladenosine. [PDF]
, 2019 Small-molecule inhibitors for the 90-kDa heat shock protein (HSP90) have been extensively exploited in preclinical studies for the therapeutic interventions of human diseases accompanied with proteotoxic stress.
Chen, Xuemei +5 more
core +1 more source