Results 151 to 160 of about 20,124 (257)

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

Synthesis and Characterization of Thermostable Antimicrobial Peptide-DNAzyme Conjugates for the Proof-of-Concept Detection of <i>E. coli</i>. [PDF]

ACS Omega
Mutter N, Savini F, Ban Ž, Saftić DP, Hloušek-Kasun A, Ahmadi Y, Bertoša B, Piantanida I, Barišić I.   +8 more
europepmc   +1 more source

Investigating transthyretin variants H88R and I107V in amyloid priming: From destabilization to complete dissociation

The FEBS Journal, EarlyView.
Investigated mutations in transthyretin (TTR) disrupt the F87‐centered hydrophobic core that stabilizes its tetrameric structure. The mild I107V mutation weakens inter‐chain packing, while H88R fully abolishes tetramer formation, yielding a monomeric, aggregation‐prone form. Structural, biophysical, and computational analyses reveal that both mutations
István L. Bódy, Zsolt Fazekas, Nóra Wágner, Anna J. Kiss‐Szemán, Veronika Harmat, Zoltán Pozsonyi, Dóra K. Menyhárd, András Perczel   +7 more
wiley   +1 more source

Highly efficient expression of DNA-peptide conjugates in growth-arrested cells. [PDF]

Nat Commun
Mohamedshah ZY, Chi CC, Tota EM, Komor AC, Devaraj NK.   +4 more
europepmc   +1 more source

Cryo‐EM structures of multiple‐peptide resistance factor (MprF) from Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
Cryo‐EM analysis of MprF from Pseudomonas aeruginosa reveals a dimeric enzyme, distinct from Rhizobium homologues. The soluble GNAT domain contains a dynamic amphipathic helix (TTH) that adopts different positions in detergent micelle and nanodisc, relative to the membrane.
Shaileshanand Jha, Kutti R. Vinothkumar
wiley   +1 more source

Improving the Cellular Accumulation of Folate-Conjugated Fully Chemically Modified siRNAs via 3' Terminal Conjugation. [PDF]

ACS Omega
Motosawa K, Iwano J, Harumoto T, Yabuuchi H, Hatanaka K, Koda Y, Kubo T, Kodaira H, Uehara K.   +8 more
europepmc   +1 more source

ALOX15 mediates thrombin‐induced myristoylation, trafficking, and interaction of protease‐activated receptors, leading to platelet activation and hemostasis

The FEBS Journal, EarlyView.
The role of PARs in platelet function is fairly known; however, the underlying mechanisms are not fully understood. We report that thrombin myristoylates PAR4 in mouse platelets and PAR1 and PAR4 in human platelets, facilitating their membrane transport and interaction, leading to PAR3/4 heterodimers in mice and PAR1/4 heterodimers in humans.
Suresh Govatati, Chandra Sekhar Yadavalli, Md Monirul Hoque, Swapna Thota, Gadiparthi N. Rao   +4 more
wiley   +1 more source

Harnessing oligonucleotide architecture for potent multivalent inhibition of human carbonic anhydrases. [PDF]

RSC Med Chem
Abbass EM, Ladu F, Mansot J, Noël M, Nocentini A, Sechi M, Supuran CT, Smietana M, Winum JY.   +8 more
europepmc   +1 more source

Aptamer‐mediated outer membrane destabilization overcomes low permeability resistance in Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
DNA aptamers, Apt 60 and Apt 173, target the BamA protein to compromise outer membrane integrity in Pseudomonas aeruginosa, making it susceptible to Azithromycin, an antibiotic that is otherwise poorly effective due to the low‐permeability membrane.
Rupany Selvam, Michelle Khai Khun Yap, Rafael Giacomazzi, Sérgio F. Sousa, Hock Siew Tan   +4 more
wiley   +1 more source

Advances in antisense oligonucleotide treatment for cancer. [PDF]

Jpn J Clin Oncol
Zhu L, Muhtar P, Goyama S, Yoshimi A.
europepmc   +1 more source