Results 151 to 160 of about 723,122 (201)
Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics
Interdisciplinary Medicine, EarlyView.This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li +7 more
wiley +1 more source
Ion Activation Methods for Top‐Down Proteomics
Mass Spectrometry Reviews, EarlyView.ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
Mass Spectrometry Reviews, EarlyView.ABSTRACT Top‐down proteomics (TDP) characterizes proteoforms in cells, tissues, and biofluids, in discovery mode and on a global scale, requiring analytical tools with high peak capacity for proteoform separation and high sensitivity for proteoform detection, given the extremely high proteoform complexity and wide proteoform concentration dynamic range.
Guijie Zhu +5 more
wiley +1 more source
Molecular Carcinogenesis, EarlyView.ABSTRACT Long noncoding RNAs (lncRNAs) play an important role in tumor progression. However, most of the previously discovered lncRNAs that promote or inhibit tumor progression were derived from bulk RNA‐seq results. Data from single‐cell RNA sequencing (scRNA‐seq) and single‐cell assay for transposase‐accessible chromatin using sequencing (scATAC‐seq)
Jizhen Cen +12 more
wiley +1 more source
KAT8‐Low Osteosarcoma Suppresses Cisplatin Sensitivity via H4K16ac‐Mediated Cell Apoptosis
Med Research, EarlyView.The scientific significance of this study is highlighted below: (1) Data mining and clinical discovery: These findings establish a direct clinical link between the KAT8–H4K16ac axis and chemotherapy response in osteosarcoma. (2) Multi‐omics and machine‐learning integration: We integrated proteomics and CUT&Tag multi‐omics to elucidate how chromatin ...
Zhenqun Zhao +12 more
wiley +1 more source
Organoid Coculture Models for Cancer Research and Immunotherapy
Med Research, EarlyView.This review summarizes organoid coculture platforms, including submerged Matrigel culture, air–liquid interface, microfluidic/organoid‐on‐a‐chip, and 3D bioprinting, for reconstructing the tumor microenvironment to study tumor–stroma/immune crosstalk, evaluate immunotherapies, and enable drug screening.
Zhuo Yang +9 more
wiley +1 more source
Medicinal Research Reviews, EarlyView.ABSTRACT Targeted elimination of pathogenic proteins remains a fundamental challenge in therapeutic intervention, particularly for proteins considered “undruggable” by conventional inhibition‐based approaches. Aptamer–based targeted protein degradation (TPD) has emerged as a versatile strategy that leverages the high specificity, structural ...
Ambrin Malik +3 more
wiley +1 more source
ISAba1‐mediated crp‐osmC amplification enhances host‐associated fitness in Acinetobacter baumannii
mLife, EarlyView.Abstract Acinetobacter baumannii is a pathogen, highly adaptable to both hospital‐ and host‐associated environments. Understanding the genetic mechanisms underlying its adaptability is critical for controlling its persistence. Our study identified the crp‐osmC gene cluster, flanked by ISAba1 elements, as a significant genetic structure undergoing ...
Da‐Wei Wei +7 more
wiley +1 more source
Small, EarlyView.Structure‐guided internal twin‐Strep tagging of the tetraspanins CD63 or CD9 enables rapid and efficient antibody‐free affinity isolation of engineered extracellular vesicles (EVs) using Strep technology while allowing luminal protein loading. The approach removes over 96% of contaminants, separates tagged EVs from coexisting viral particles, and ...
Minh‐Tu Pham +14 more
wiley +1 more source
Small, EarlyView.A three‐residue peptide bioconjugated onto a carbon nanotube quantum defect turns the near‐infrared emission of quantum defects into a reporter of integrins on intact extracellular vesicles, directly in human plasma. The sensor reveals that healthy fibroblast vesicles carry as much RGD‐binding integrin as cancer‐derived ones, an adhesion‐associated ...
In‐Jun Hwang +9 more
wiley +1 more source