Results 91 to 100 of about 7,628 (191)

Enhancing Clinical Detection Accuracy of Large Structured Viral RNA via DNAzyme Cleavage and Antisense‐Assisted Rolling Circle Amplification

open access: yesAngewandte Chemie, Volume 137, Issue 33, August 11, 2025.
We show that antisense oligonucleotides can be used to restructure secondary structural elements around the circular DNA template binding sites of the genomic RNA for subsequent rolling circle amplification. This strategy was employed to identify otherwise COVID “false negative” saliva samples by accurately detecting the presence of SARS‐CoV‐2 genomic ...
Amal Mathai   +4 more
wiley   +2 more sources

Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth. [PDF]

open access: yes, 2021
The lack of efficient tools to label multiple endogenous targets in cell lines without staining or fixation has limited our ability to track physiological and pathological changes in cells over time via live-cell studies.

core   +1 more source

The neutrophil‐to‐lymphocyte ratio as a marker of immunosenescence and COVID‐19 outcomes in the elderly: A narrative review

open access: yesPhysiological Reports, Volume 14, Issue 3, February 2026.
Abstract Older adults are highly vulnerable to severe COVID‐19. Unlike our previous work on broad immunosenescence, this review focuses on peripheral hematological markers as practical indicators of risk. To examine lymphopenia, neutrophilia, and the neutrophil‐to‐lymphocyte ratio (NLR) as clinically accessible markers of immune aging and COVID‐19 ...
Maha Gasmi   +4 more
wiley   +1 more source

Evaluation of Antigen Expression and Early Immune Response Following Cutaneous Suction‐Mediated DNA Delivery

open access: yesAdvanced Therapeutics, Volume 9, Issue 1, January 2026.
In vivo suction‐based cutaneous DNA vaccination promotes robust immunogenic responses. The spatiotemporal patterns of antigen expression following suction‐based SARS‐CoV‐2 DNA vaccine delivery are evaluated in rat skin via immunofluorescence staining.
Nandita C. Jhumur   +12 more
wiley   +1 more source

HIF-1α promotes SARS-CoV-2 infection and aggravates inflammatory responses to COVID-19

open access: yesSignal Transduction and Targeted Therapy, 2021
Cytokine storm induced by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a major pathological feature of Coronavirus Disease 2019 (COVID-19) and a crucial determinant in COVID-19 prognosis. Understanding the mechanism underlying the SARS-
Mingfu Tian   +17 more
doaj   +1 more source

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells

open access: yesiScience, 2021
Summary: We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of ...
Thushan I. de Silva   +1012 more
doaj   +1 more source

Tetherin antagonism by SARS-CoV-2 ORF3a and spike protein enhances virus release [PDF]

open access: yes, 2023
The antiviral restriction factor, tetherin, blocks the release of several different families of enveloped viruses, including the Coronaviridae. Tetherin is an interferon‐induced protein that forms parallel homodimers between the host cell and viral ...
Brown, Jonathan C   +14 more
core  

Genomic and Phylogenetic Dissection of SARS‐CoV‐2 Transmission Networks in Healthcare Workers

open access: yesInternational Journal of Microbiology, Volume 2026, Issue 1, 2026.
Understanding how SARS‐CoV‐2 circulates among healthcare workers (HCWs) is critical for maintaining safe hospitals and protecting communities. We retrospectively examined 93 archived nasopharyngeal swabs collected from SARS‐CoV‐2 positive HCWs and their close contacts at a Turkish university hospital between March 2020 and November 2021.
Irmak Güzel   +10 more
wiley   +1 more source

Unique and complementary suppression of cGAS-STING and RNA sensing- triggered innate immune responses by SARS-CoV-2 proteins

open access: yesSignal Transduction and Targeted Therapy, 2021
The emergence of SARS-CoV-2 has resulted in the COVID-19 pandemic, leading to millions of infections and hundreds of thousands of human deaths. The efficient replication and population spread of SARS-CoV-2 indicates an effective evasion of human innate ...
Yajuan Rui   +15 more
doaj   +1 more source

Home - About - Disclaimer - Privacy