Results 11 to 20 of about 6,344 (144)

Variations in Orf3a protein of SARS-CoV-2 alter its structure and function

Biochemistry and Biophysics Reports, 2021
Severe acquired respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spread worldwide and acquired multiple mutations in its genome. Orf3a, an accessory protein encoded by the genome of SARS-CoV-2, plays a significant role in viral infection and ...
Gajendra Kumar Azad, Parimal Kumar Khan
exaly   +3 more sources

Reprogramming of lipid metabolism by ORF3a-induced microlipophagy enhances biogenesis of SARS-CoV-2 replication organelle. [PDF]

PLoS Pathog
Infection by positive-strand RNA viruses necessitates membrane expansion and elevated phospholipid biosynthesis, whereby fatty acids stored as triacylglycerols in lipid droplets (LDs) are mobilized to promote metabolic processes and membrane biogenesis ...
Li Z, Hui X, Zheng M, He Z, Huai W, Ding B, Jin M, Qin Y, Chen M.   +8 more
europepmc   +2 more sources

The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins

eLife, 2023
The severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) and SARS-CoV-1 accessory protein Orf3a colocalizes with markers of the plasma membrane, endocytic pathway, and Golgi apparatus.
Alexandria N Miller, Patrick R Houlihan, Ella Matamala, Deny Cabezas-Bratesco, Gi Young Lee, Ben Cristofori-Armstrong, Tanya L Dilan, Silvia Sanchez-Martinez, Doreen Matthies, Rui Yan, Zhiheng Yu, Dejian Ren, Sebastian E Brauchi, David E Clapham   +13 more
doaj   +2 more sources

SARS-CoV-2 virulence factor ORF3a blocks lysosome function by modulating TBC1D5-dependent Rab7 GTPase cycle

Nature Communications
SARS-CoV-2, the causative agent of COVID-19, uses the host endolysosomal system for entry, replication, and egress. Previous studies have shown that the SARS-CoV-2 virulence factor ORF3a interacts with the lysosomal tethering factor HOPS complex and ...
Kshitiz Walia, Abhishek Sharma, Sankalita Paul, Priya Chouhan, Gaurav Kumar, Rajesh Ringe, Mahak Sharma, Amit Tuli   +7 more
doaj   +2 more sources

SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity. [PDF]

Nat Commun
SARS-CoV-2 hijacks multiple organelles for virion assembly, of which the mechanisms have not been fully understood. Here, we identified a SARS-CoV-2-driven membrane structure named the 3a dense body (3DB).
Hartmann S, Radochonski L, Ye C, Martinez-Sobrido L, Chen J.   +4 more
europepmc   +3 more sources

ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress

Developmental Cell, 2021
Viral entry and egress are important determinants of virus infectivity and pathogenicity. β-coronaviruses, including the COVID-19 virus SARS-CoV-2 and mouse hepatitis virus (MHV), exploit the lysosomal exocytosis pathway for egress.
Qiaoxia Zheng, Long Sun, Mingming Ji
exaly   +2 more sources

The role of JNK in the proinflammatory action of the SARS-CoV-2 protein ORF3a [PDF]

Medicinski Podmladak
Introduction: Coronavirus disease 2019 (COVID-19) is a contagious respiratory infection caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Aranđelović Lena, Mandić Miloš, Vučićević Ljubica, Misirkić Marjanović Maja, Trajković Vladimir   +4 more
doaj   +2 more sources

Activity and cellular distribution of ORF3a mutants of SARS-CoV-2 variants of concern. [PDF]

J Gen Virol
Infection with SARS-CoV-2 continues to be a threat to human health. Despite successful immunization campaigns, effective treatment of COVID-19 remains an essential need to help patients and prevent the spread of new virus strains.
Breitinger U, Zakaria ZIS, Mahgoub HA, Wiessler AL, Tuerker E, Villmann C, Breitinger HG.   +6 more
europepmc   +2 more sources

SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathway

Virology, 2022
Heightened inflammatory response is a prominent feature of severe COVID-19 disease. We report that the SARS-CoV-2 ORF3a viroporin activates the NLRP3 inflammasome, the most promiscuous of known inflammasomes.
Huanzhou Xu, Ibukun A Akinyemi, Julia C Loeb   +2 more
exaly   +2 more sources

Examining the feasibility of replacing ORF3a with fluorescent genes to construct SARS-CoV-2 reporter viruses. [PDF]

J Gen Virol
The SARS-CoV-2 genome encodes at least nine accessory proteins, including innate immune antagonist and putative viroporin ORF3a. ORF3a plays a role in many stages of the viral replication cycle, including immune modulation. We constructed two recombinant
Webb I, Erdmann M, Milligan R, Savage M, Matthews DA, Davidson AD.   +5 more
europepmc   +2 more sources