Results 31 to 40 of about 6,597 (167)

A Recent Origin of Orf3a From M Protein Across the Coronavirus Lineage Arising by Sharp Divergence

Computational and Structural Biotechnology Journal, 2020
The genome of SARS-CoV-2, the coronavirus responsible for the Covid-19 pandemic, encodes a number of accessory genes. The longest accessory gene, Orf3a, plays important roles in the virus lifecycle indicated by experimental findings, known polymorphisms,
Christos Ouzounis
exaly   +3 more sources

Inhibition of PERK Kinase, an Orchestrator of the Unfolded Protein Response (UPR), Significantly Reduces Apoptosis and Inflammation of Lung Epithelial Cells Triggered by SARS-CoV-2 ORF3a Protein

Biomedicines, 2023
SARS-CoV-2 ORF3a accessory protein was found to be involved in virus release, immunomodulation and exhibited a pro-apoptotic character. In order to unravel a potential ORF3a-induced apoptotic and inflammatory death mechanism, lung epithelial cells (A549)
Panagiotis Keramidas, Eleni Papachristou, Rigini M. Papi, Aglaia Mantsou, Theodora Choli-Papadopoulou   +4 more
doaj   +2 more sources

SARS-CoV-2 ORF3a accessory protein is a water-permeable channel that induces lysosome swelling [PDF]

Communications Biology
ORF3a, the most abundantly expressed accessory protein of SARS-CoV-2, plays an essential role in virus egress by inactivating lysosomes through their deacidification. However, the mechanism underlying this process remains unclear.
Antonio Michelucci, Luigi Sforna, Riccardo Focaia, Maria Vittoria Leonardi, Angela Di Battista, Giorgia Rastelli, Simone Vespa, Simona Boncompagni, Manlio Di Cristina, Luigi Catacuzzeno   +9 more
doaj   +2 more sources

SARS-CoV-2 Orf3a protein interaction mapping using unnatural amino acid incorporation. [PDF]

Protein Eng Des Sel
Mapping transient protein–protein interactions remain a major challenge in studying viral host–pathogen interfaces. While some virus-host interactions are stable and readily captured, the majority are highly dynamic, reflecting the need for a small ...
Lundrigan E, Ahmed N, Pezacki JP.
europepmc   +4 more sources

Reprogramming of lipid metabolism by ORF3a-induced microlipophagy enhances biogenesis of SARS-CoV-2 replication organelle. [PDF]

PLoS Pathogens
Infection by positive-strand RNA viruses necessitates membrane expansion and elevated phospholipid biosynthesis, whereby fatty acids stored as triacylglycerols in lipid droplets (LDs) are mobilized to promote metabolic processes and membrane biogenesis ...
Zhifei Li, Xianfeng Hui, Miaomiao Zheng, Zhicheng He, Wenjing Huai, Binbin Ding, Meilin Jin, Yali Qin, Mingzhou Chen   +8 more
doaj   +2 more sources

SARS-CoV-2 ORF3a induces COVID-19-associated kidney injury through HMGB1-mediated cytokine production

mBio
The primary challenge posed by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is COVID-19-related mortality, often exacerbated by additional medical complications, such as COVID-19-associated kidney injuries (CAKIs). Up to half of
Chenyu Zhang, Volodymyr Gerzanich, Ruth Cruz-Cosme, Jiantao Zhang, Orest Tsymbalyuk, Cigdem Tosun, Bhargava Teja Sallapalli, Dongxiao Liu, Kaspar Keledjian, John C. Papadimitriou, Cinthia B. Drachenberg, Mohamed Nasr, Yanjin Zhang, Qiyi Tang, J. Marc Simard, Richard Y. Zhao   +15 more
doaj   +2 more sources

Proteome profiling of human lung epithelial cells unveils distinct patterns of protein expression in response to SARS-CoV-2 ORF3a

The Microbe
The COVID-19 pandemic caused by SARS-CoV-2 remains an ongoing global health concern. Understanding the roles of SARS-CoV-2 accessory proteins is essential for developing effective therapeutic strategies.
Apoorva, Astha Shukla, Atul Kumar, Saurabh Singh, Sunit K. Singh   +4 more
doaj   +2 more sources

Introduction of ORF3a-Q57H SARS-CoV-2 Variant Causing Fourth Epidemic Wave of COVID-19, Hong Kong, China [PDF]

Emerging Infectious Diseases, 2021
We describe an introduction of clade GH severe acute respiratory syndrome coronavirus 2 causing a fourth wave of coronavirus disease in Hong Kong. The virus has an ORF3a-Q57H mutation, causing truncation of ORF3b. This virus evades induction of cytokine,
Daniel K.W. Chu, Kenrie P.Y. Hui, Haogao Gu, Ronald L.W. Ko, Pavithra Krishnan, Daisy Y.M. Ng, Gigi Y.Z. Liu, Carrie K.C. Wan, Man-Chun Cheung, Ka-Chun Ng, John M. Nicholls, Dominic N.C. Tsang, Malik Peiris, Michael C.W. Chan, Leo L.M. Poon   +14 more
doaj   +2 more sources

Novel Vaccines Targeting the Highly Conserved SARS-CoV-2 ORF3a Ectodomain Elicit Immunogenicity in Mouse Models

Vaccines
Background: The majority of antigen-based SARS-CoV-2 (SCV2) vaccines utilized in the clinic have had the Spike protein or domains thereof as the immunogen.
Jacob Meza, Elizabeth Glass, Avinaash K. Sandhu, Yangchen Li, Styliani Karanika, Kaitlyn Fessler, Yinan Hui, Courtney Schill, Tianyin Wang, Jiaqi Zhang, Rowan E. Bates, Alannah D. Taylor, Aakanksha R. Kapoor, Samuel K. Ayeh, Petros C. Karakousis, Richard B. Markham, James T. Gordy   +16 more
doaj   +2 more sources

SARS-CoV-2 enhances lysosomal exocytosis and deacidifies lysosomes to facilitate viral release. [PDF]

mLife
Abstract The mechanism of SARS‐CoV‐2 egress predominantly governs the quantity and quality of progeny viruses, thereby significantly contributing to viral pathogenicity. However, the key factors influencing viral egress remain largely unclear. In this study, using transcription‐ and replication‐competent SARS‐CoV‐2 virus‐like‐particle (SARS‐CoV‐2 trVLP)
Qin F, Yan C, Liu Z, Wang D, Zhong H, Ding Q, Chen M, Zhang XE.   +7 more
europepmc   +2 more sources