Results 1 to 10 of about 85,597 (305)

Effect of the Matrix and Target on the Accurate Quantification of Genomic and Plasmid DNA by Digital Polymerase Chain Reaction

open access: yesAgriculture, 2023
In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) and digital PCR (dPCR) affect the accuracy and reliability of ...
Nengwu Si   +8 more
doaj   +1 more source

A Label-Free Electrochemical Impedance Genosensor Coupled with Recombinase Polymerase Amplification for Genetically Modified Maize Detection

open access: yesAgriculture, 2022
As the cultivation scale of genetically modified (GM) crops strongly increases, a convenient DNA assay is highly demanded in resource-limited areas. A label-free electrochemical impedance (EI) genosensor using gold carbon dots (GCDs) was developed with ...
Dandan Cui   +8 more
doaj   +1 more source

Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example

open access: yesFood Chemistry: Molecular Sciences, 2022
Basic data for the safety assessment of transgenic line involves the molecular characterization of the integration site of exogenous DNA, flanking sequences, copy number, and unintended plasmid backbone residues.
Hanwen Zhang   +5 more
doaj   +1 more source

Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation

open access: yesEcotoxicology and Environmental Safety, 2023
2,4-Dinitrotoluene (2,4-DNT) as a common industrial waste has been massively discharged into the environment with industrial wastewater. Due to its refractory degradation, high toxicity, and bioaccumulation, 2,4-DNT pollution has become increasingly ...
Wen-Hui Zhang   +14 more
doaj   +1 more source

A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

open access: yesCommunications Biology, 2022
Coupling paired-end whole-genome sequencing with droplet digital PCR enabled precise identification of a transgene insertion in the genetically modified rice event G281 on chromosome 3 and the potential for exploring the native gene integration.
Wenting Xu   +6 more
doaj   +1 more source

Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

open access: yesFoods, 2022
Certified reference materials (CRMs) is one of the critical requirements in a quantitative analytical method, such as in the quantification of genetically modified (GM) contents in food/feed products.
Yanan Meng   +3 more
doaj   +1 more source

The Genetically Modified Organism Conflict [PDF]

open access: yesPlant Physiology, 2000
Few readers of this journal will have failed to notice the recent uproar, particularly in Great Britain, concerning the release of genetically modified organisms (GMOs). In response to the demands of activists, European governments have restricted the import and release of GMOs, and activists ...
openaire   +2 more sources

Scientific Opinion on a request from the European Commission related to the prolongation of prohibition of the placing on the market of genetically modified oilseed rape event GT73 for import, processing and feed uses in Austria [PDF]

open access: yesEFSA Journal, 2013
Following a request from the European Commission, the Panel on Genetically Modified Organisms of the European Food Safety Authority (EFSA GMO Panel) evaluated the documentation provided by Austria to support the prolongation of the safeguard clause ...
EFSA Panel on Genetically Modified Organisms (GMO)
doaj   +1 more source

Scientific Opinion on a request from the European Commission related to the safeguard clause notified by Greece on genetically modified maize MON 810 according to Article 23 of Directive 2001/18/EC [PDF]

open access: yesEFSA Journal, 2012
<p>Following a request of the European Commission, the European Food Safety Authority’s Panel on Genetically Modified Organisms (EFSA GMO Panel) evaluated the documentation submitted by Greece in support of its request for the prohibition of the ...
EFSA Panel on Genetically Modified Organisms (GMO)
doaj   +1 more source

Scientific Opinion on application (EFSA-GMO-NL-2010-78) for the placing on the market of herbicide-tolerant, increased oleic acid genetically modified soybean MON 87705 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto [PDF]

open access: yesEFSA Journal, 2012
<p>This scientific opinion is a risk assessment of the genetically modified, herbicide-tolerant, increased oleic acid soybean MON 87705 for food and feed uses, import and processing.
EFSA Panel on Genetically Modified Organisms (GMO)
doaj   +1 more source

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