Results 21 to 30 of about 8,453 (201)

Dynamics of nuclear export of pre-ribosomal subunits revealed by high-speed single-molecule microscopy in live cells

iScience, 2023
Summary: We present a study on the nuclear export efficiency and time of pre-ribosomal subunits in live mammalian cells, using high-speed single-molecule tracking and single-molecule fluorescence resonance energy transfer techniques.
Samuel L. Junod, Mark Tingey, Joseph M. Kelich, Alexander Goryaynov, Karl Herbine, Weidong Yang   +5 more
doaj   +1 more source

Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages

iScience, 2022
Summary: Podosomes, an important actin-based adhesive architecture, play critical roles in cell migration and matrix invasiveness. Here, we elucidate the ultrastructural organization and regulation of podosome clusters in primary macrophages.
Fen Hu, Donglan Zhu, Hao Dong, Ping Zhang, Fulin Xing, Wan Li, Rui Yan, Jun Zhou, Ke Xu, Leiting Pan, Jingjun Xu   +10 more
doaj   +1 more source

Hsp70 exhibits a liquid-liquid phase separation ability and chaperones condensed FUS against amyloid aggregation

iScience, 2022
Summary: Hsp70 is a key molecular chaperone in the protein quality control system to safeguard protein homeostasis in cells. Previous studies have shown that Hsp70 chaperones TDP-43, a pathogenic protein associated with amyotrophic lateral sclerosis (ALS)
Yichen Li, Jinge Gu, Chen Wang, Jiaojiao Hu, Shenqing Zhang, Cong Liu, Shengnan Zhang, Yanshan Fang, Dan Li   +8 more
doaj   +1 more source

Infection Control Practices for Vascular Access Management in Hemodialysis: Results From a Nationwide Survey of Japanese National University Hospitals

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Bloodstream infections due to repeated vascular access (VA) puncture and circuit connections remain major concerns in hemodialysis. Therefore, we examined current practices for glove, disinfectant, and personal protective equipment (PPE) use according to VA type in national university hospitals in Japan.
Aiko Yamada, Makoto Harada, Tadahiko Kikugawa, Takeshi Nakata, Koji Hashimoto, Tsuneo Konta, Yuji Kamijo   +6 more
wiley   +1 more source

A nucleation distance sets the cell size-dependent P-body assembly in yeast

iScience
Summary: Cells rely on diverse intracellular structures, including phase-separated granules, to regulate biological processes. Processing bodies (P-bodies) are membraneless cytoplasmic condensates that mediate mRNA degradation and storage. Although their
Xuefang Gu, Hamza El Alami, Dexin Shen, Xing Quan, Tatsuhisa Tsuboi   +4 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics

iScience
Summary: Lamellipodia are sheet-like protrusions essential for cell migration and endocytosis, but their ultrastructural dynamics remain poorly understood because conventional electron microscopy lacks temporal resolution.
Hironori Inaba, Tsuyoshi Imasaki, Kazuhiro Aoyama, Shogo Yoshihara, Hiroko Takazaki, Takayuki Kato, Hidemasa Goto, Kaoru Mitsuoka, Ryo Nitta, Takao Nakata   +9 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Myosin cluster dynamics determines epithelial wound ring constriction

iScience
Summary: Collection of myosin motors and actin filaments can self-assemble into submicrometric clusters under the regulation of RhoA. Emergent dynamics of these clusters have been reported in a variety of morphogenetic systems, ranging from Drosophila to
Alka Bhat, Rémi Berthoz, Simon Lo Vecchio, Coralie Spiegelhalter, Shigenobu Yonemura, Olivier Pertz, Daniel Riveline   +6 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source