Results 41 to 50 of about 81,677 (304)

RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variability

Life Science Alliance, 2022
Over the last decades, organoids have been established from most of the tissue-resident stem and iPS cells. They hold great promise for our understanding of mammalian organ development, but also for the study of disease or even personalised medicine. In recent years, several reports hinted at intraculture organoid variability, but a systematic analysis
Kristin Gehling, Swati Parekh, Farina Schneider, Marcel Kirchner, Vangelis Kondylis, Chrysa Nikopoulou, Peter Tessarz   +6 more
openaire   +3 more sources

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

A brief history of organoids [PDF]

American Journal of Physiology-Cell Physiology, 2020
In vitro cell cultures are crucial research tools for modeling human development and diseases. Although the conventional monolayer cell cultures have been widely used in the past, the lack of tissue architecture and complexity of such model fails to inform the true biological processes in vivo.
Claudia Corrò, Laura Novellasdemunt, Vivian S.W. Li   +2 more
openaire   +2 more sources

From organoids to gastruloids

, 2017
Last year was hailed as ‘the year of the organoid’ as news of mini- brains, livers and intestines grown in the laboratory hit the headlines. Here, Dr David Turner explains the latest in vitro systems for modelling human development and disease, including
Turner, DA
core   +1 more source

Strategies for vascularization in kidney organoids

, 2022
The establishment of protocols for differentiating kidney organoids from human pluripotent stem cells (hPSCs) has potential for the application of kidney organoids in regenerative medicine. However, the primary obstacle to the regenerative application of
Yong Kyun Kim, Seo-Yeon Park
core   +1 more source

Development of Low-Cost CNC-Milled PMMA Microfluidic Chips as a Prototype for Organ-on-a-Chip and Neurospheroid Applications

Organoids
Improved in vitro models are needed to reduce costs and delays in central nervous system (CNS) drug discovery. The FDA Modernization Acts 2.0 and 3.0 require human-centered alternative testing methods to mitigate animal-based experiments and discovery ...
Sushmita Mishra, Ginia Mondal, Murali Kumarasamy   +2 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Development of 3D culture techniques for human brain organoids derivation

, 2022
reservedLo sviluppo neuronale dell’embrione umano non può essere richiamato con modelli in 2D, perché non presentano un’appropriata citoarchitettura e una eterogenea popolazione neuronale.
FRISON, ROBERTA
core  

Inhibition of the cGAS‐STING pathway ameliorates the premature senescence hallmarks of Ataxia‐Telangiectasia brain organoids

, 2021
Ataxia-telangiectasia (A-T) is a genetic disorder caused by the lack of functional ATM kinase. A-T is characterized by chronic inflammation, neurodegeneration and premature ageing features that are associated with increased genome instability, nuclear ...
Mackay-Sim, A, Hannah C. Leeson, Gómez-Inclán, C, Leeson, HC, Alan Mackay‐Sim, Ernst J. Wolvetang, Julio Aguado, Aguado, J, Harman K. Chaggar, Shaker, MR, Mohammed R. Shaker, Cecilia Gómez‐Inclán, Chaggar, HK, Wolvetang, EJ   +13 more
core   +1 more source

Circulating Tumor Cell‐Derived Organoids: Current Progress, Applications, and Future

MedComm – Future Medicine
Circulating tumor cells (CTCs) are malignant cells that detach from primary or metastatic tumors and enter the bloodstream. Organoids, as three‐dimensional in vitro models, can mimic the tumor microenvironment and histopathological characteristics ...
Tiantian Li, Biao Deng, Sheng Li, Yanxia Wu, Zhenghao Lu, Zhu Liang   +5 more
doaj   +1 more source