Results 251 to 260 of about 7,859 (293)

Asymmetric replication of an oriC plasmid in Escherichia coli

Molecular Genetics and Genomics, 1986
Plasmid pTSO118 containing the Escherichia coli origin of replication, oriC, initiated replication simultaneously with the chromosome when temperature-sensitive host cells were synchronized by temperature shifts. Replicating intermediates of the plasmid as well as of the chromosome were isolated from the outer membrane fraction of the cell. Plasmid DNA
G Tamura, Gakuzo Tamura, Kazuo Nagai
exaly   +3 more sources

Improvements in the Oric RF System

IEEE Transactions on Nuclear Science, 1965
The Oak Ridge Isochronous Cyclotron has been operating since January 1963. Initial experience with the rf system was enlivened by destructive sparking and general instability at the upper end of the rf tuning range. A network of protective devices developed during the past two years almost eliminates spark damage and improves the stability of the ...
S. W. Mosko, N. F. Ziegler
openaire   +1 more source

Fully Methylated oriC with Negative Superhelicity Forms an oriC-Membrane Complex before Initiation of Chromosome Replication

Biochemical and Biophysical Research Communications, 1993
In an in vitro assay, the oriC DNA has been shown to bind to the outer membrane fraction only when it is hemimethylated (G.B. Ogden et al., Cell, 54, 127-135,1988). In this report, however, we demonstrated that a significant amount of the oriC DNA was recovered from the cells just before initiation with the oriC DNA being fully methylated. Formation of
Takao Kataoka, Masaaki Wachi, Jun Nakamura, Shinyo Gayama, Makari Yamasaki, Kazuo Nagai   +5 more
openaire   +2 more sources

Oric Beam Energy Increase

Proceedings of the 2005 Particle Accelerator Conference, 2006
The detection of and solution to a beam interference problem in the Oak Ridge Isochronous Cyclotron (ORIC) extraction system has yielded a 20% increase in the proton beam energy. The beam from ORIC was designed to be extracted before the v r = 1 resonance.
M.L. Mallory, J.B. Ball, D.T. Dowling, E.D. Hudson, R.S. Lord, B.A. Tatum   +5 more
openaire   +1 more source

An axial injection system for the ORIC

Proceedings of the 2003 Bipolar/BiCMOS Circuits and Technology Meeting (IEEE Cat. No.03CH37440), 2004
An axial injection system has been designed for injection of high intensity beams of H/sup -/, D/sup -/, and He/sup ++/ into the Oak Ridge Isochronous Cyclotron (ORIC). The device consists of two high-voltage platforms, two external ion sources, a double-drift-tube beam bunching system, a spiral inflector and integrated beam analysis diagnostics and ...
G.D. Alton, Y. Zhang, B.A. Taturn
openaire   +1 more source

Origin of replication, oriC, of the Escherichia coli chromosome: Mapping of genes relative to R.EcoRI cleavage sites in the oriC region

Molecular and General Genetics MGG, 1977
A precise genetic-physical map of the tna-ilv region at 82 min on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F' carrying the genes between aroE and ilv. A locus, designated het, which in its diploid state results in slow growth and heterogeneity of cell ...
von Meyenburg, K, Hansen, F G, Nielsen, L D, Jørgensen, P, Jørgensen, Poul   +4 more
openaire   +3 more sources

The complex of oriC DNA with the DnaA initiator protein

Research in Microbiology, 1991
We describe several experimental approaches relating to the early steps in the initiation of DNA replication at oriC. 1) A matrix is given which enables calculatation of the relative affinity of DnaA boxes for DnaA protein; 2) base changes within single Dna A boxes in oriC have little effect on oriC function; 3) mutations which change the distance ...
W, Messer, B, Egan, H, Gille, A, Holz, C, Schaefer, B, Woelker   +5 more
openaire   +2 more sources

Development of an oriC vector for use in Mycoplasma synoviae

Journal of Microbiological Methods, 2014
Mycoplasma synoviae, an important poultry pathogen, belonging to the class Mollicutes, causes airsacculitis, synovitis, decreased egg production and produces significant economic losses. Efforts to determine M. synoviae virulence factors and their role in pathogenicity require suitable tools for genetic manipulation of this pathogen.
Muhammad A, Shahid, Marc S, Marenda, Philip F, Markham, Amir H, Noormohammadi   +3 more
openaire   +2 more sources

Magnet regulators for ORIC

Nuclear Instruments and Methods, 1962
Twenty-one current regulator systems were built, tested, and put in operation in the Oak Ridge Isochronous Cyclotron. Two motor-generator units rated at 800 and 1200 kW are regulated by transistor banks in the field circuits of the generators. Nineteen other magnet supplies are rectifier-magnetic amplifier systems with ratings ranging from 6 to 30 kW ...
openaire   +1 more source

dam methylation and the initiation of DNA replication on oriC plasmids

Molecular Genetics and Genomics, 1989
Plasmid DNA containing the replication origin of the Escherichia coli chromosome (oriC) has been shown to be inefficient as a template for DNA synthesis in vitro when isolated from dam mutants. Here, we extend this study to hemimethylated oriC plasmids and to replication in dam-3 mutant enzyme extracts.
Ahmed Landoulsi, Masamichi Kohiyama, R Kern   +2 more
exaly   +3 more sources