Results 251 to 260 of about 7,205 (295)
Plasmid Curing of <i>Pseudoalteromonas haloplanktis</i> TAC125 Using Homologous Recombination and PTasRNA Gene Silencing. [PDF]
Bio ProtocSeverino A +4 more
europepmc +1 more source
Some of the next articles are maybe not open access.
Related searches:
Related searches:
Negative control of oriC plasmid replication by transcription of the oriC region
Molecular Genetics and Genomics, 1985We have demonstrated that the replication of the oriC plasmid, carrying the replication origin of the Escherichia coli chromosome, is inhibited by transcriptional readthrough from an oriC flanking region of the plasmid. This was drawn from an examination of the replication of an oriC plasmid, pKZ4, which bears the lacOP segment at the right-hand side ...
S Hiraga, Hiraga Sota
exaly +3 more sources
dam methylation and the initiation of DNA replication on oriC plasmids
Molecular Genetics and Genomics, 1989Plasmid DNA containing the replication origin of the Escherichia coli chromosome (oriC) has been shown to be inefficient as a template for DNA synthesis in vitro when isolated from dam mutants. Here, we extend this study to hemimethylated oriC plasmids and to replication in dam-3 mutant enzyme extracts.
Ahmed Landoulsi +2 more
exaly +3 more sources
Biochemical and Biophysical Research Communications, 1993
In an in vitro assay, the oriC DNA has been shown to bind to the outer membrane fraction only when it is hemimethylated (G.B. Ogden et al., Cell, 54, 127-135,1988). In this report, however, we demonstrated that a significant amount of the oriC DNA was recovered from the cells just before initiation with the oriC DNA being fully methylated. Formation of
Takao Kataoka +5 more
openaire +2 more sources
In an in vitro assay, the oriC DNA has been shown to bind to the outer membrane fraction only when it is hemimethylated (G.B. Ogden et al., Cell, 54, 127-135,1988). In this report, however, we demonstrated that a significant amount of the oriC DNA was recovered from the cells just before initiation with the oriC DNA being fully methylated. Formation of
Takao Kataoka +5 more
openaire +2 more sources
Molecular and General Genetics MGG, 1977
A precise genetic-physical map of the tna-ilv region at 82 min on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F' carrying the genes between aroE and ilv. A locus, designated het, which in its diploid state results in slow growth and heterogeneity of cell ...
K, von Meyenburg +3 more
openaire +4 more sources
A precise genetic-physical map of the tna-ilv region at 82 min on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F' carrying the genes between aroE and ilv. A locus, designated het, which in its diploid state results in slow growth and heterogeneity of cell ...
K, von Meyenburg +3 more
openaire +4 more sources
Proceedings of the 2005 Particle Accelerator Conference, 2006
The detection of and solution to a beam interference problem in the Oak Ridge Isochronous Cyclotron (ORIC) extraction system has yielded a 20% increase in the proton beam energy. The beam from ORIC was designed to be extracted before the v r = 1 resonance.
M.L. Mallory +5 more
openaire +1 more source
The detection of and solution to a beam interference problem in the Oak Ridge Isochronous Cyclotron (ORIC) extraction system has yielded a 20% increase in the proton beam energy. The beam from ORIC was designed to be extracted before the v r = 1 resonance.
M.L. Mallory +5 more
openaire +1 more source
An axial injection system for the ORIC
Proceedings of the 2003 Bipolar/BiCMOS Circuits and Technology Meeting (IEEE Cat. No.03CH37440), 2004An axial injection system has been designed for injection of high intensity beams of H/sup -/, D/sup -/, and He/sup ++/ into the Oak Ridge Isochronous Cyclotron (ORIC). The device consists of two high-voltage platforms, two external ion sources, a double-drift-tube beam bunching system, a spiral inflector and integrated beam analysis diagnostics and ...
G.D. Alton, Y. Zhang, B.A. Taturn
openaire +1 more source
Improvements in the Oric RF System
IEEE Transactions on Nuclear Science, 1965The Oak Ridge Isochronous Cyclotron has been operating since January 1963. Initial experience with the rf system was enlivened by destructive sparking and general instability at the upper end of the rf tuning range. A network of protective devices developed during the past two years almost eliminates spark damage and improves the stability of the ...
S. W. Mosko, N. F. Ziegler
openaire +1 more source
Asymmetric replication of an oriC plasmid in Escherichia coli
Molecular and General Genetics MGG, 1986Plasmid pTSO118 containing the Escherichia coli origin of replication, oriC, initiated replication simultaneously with the chromosome when temperature-sensitive host cells were synchronized by temperature shifts. Replicating intermediates of the plasmid as well as of the chromosome were isolated from the outer membrane fraction of the cell. Plasmid DNA
M, Yoshimoto, K, Nagai, G, Tamura
openaire +2 more sources