Rethinking origin licensing [PDF]
eLife, 2017 Human cells that lack a subunit in their origin recognition complex are viable, which suggests the existence of alternative mechanisms to initiate DNA replication.
Stephen P Bell
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Mobile origin-licensing factors confer resistance to conflicts with RNA polymerase
Cell Reports, 2022 Summary: Fundamental to our understanding of chromosome duplication is the idea that replication origins function both as sites where MCM helicases are loaded during the G1 phase and where synthesis begins in S phase.
Karl E Duderstadt +2 more
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AlphaFold-guided phylogenetic analyses suggest surprising heterogeneity in metazoan replication origin licensing mechanisms [PDF]
EMBO JournalDNA replication initiation is a tightly regulated process that requires the coordinated assembly of replication machineries throughout the genome. During the first step of initiation, origin licensing, the MCM replicative helicase motor is loaded onto ...
Franziska Bleichert
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Rapid DNA replication origin licensing protects stem cell pluripotency [PDF]
eLife, 2017 Complete and robust human genome duplication requires loading minichromosome maintenance (MCM) helicase complexes at many DNA replication origins, an essential process termed origin licensing. Licensing is restricted to G1 phase of the cell cycle, but G1
Jacob Peter Matson +10 more
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Efficiency and equity in origin licensing to ensure complete DNA replication [PDF]
Biochemical Society Transactions, 2021 The cell division cycle must be strictly regulated during both development and adult maintenance, and efficient and well-controlled DNA replication is a key event in the cell cycle. DNA replication origins are prepared in G1 phase of the cell cycle in a process known as origin licensing which is essential for DNA replication initiation in the ...
Liu Mei, Jeanette Gowen Cook
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A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6 [PDF]
Nature Communications, 2022 Cryo-EM structures of S. cerevisiae ORC bound to DNA and Cdc6 reveal an autoinhibited conformation and suggest a mechanism of origin licensing control in response to CDK phosphorylation.
Jan Marten Schmidt +5 more
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Nucleosome-directed replication origin licensing independent of a consensus DNA sequence [PDF]
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
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Human CST suppresses origin licensing and promotes AND-1/Ctf4 chromatin association [PDF]
Life Science Alliance, 2019 This study suggests a novel role of the telomere-associated human CST complex in suppressing origin licensing, through interactions with MCM, while promoting the recruitment of AND-1 and DNA polymerase alpha for replisome formation. Human CTC1-STN1-TEN1 (
Yilin Wang +4 more
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Cdc6 is sequentially regulated by PP2A-Cdc55, Cdc14, and Sic1 for origin licensing in S. cerevisiae [PDF]
eLife, 2022 Cdc6, a subunit of the pre-replicative complex (pre-RC), contains multiple regulatory cyclin-dependent kinase (Cdk1) consensus sites, SP or TP motifs. In Saccharomyces cerevisiae, Cdk1 phosphorylates Cdc6-T7 to recruit Cks1, the Cdk1 phospho-adaptor in S
Jasmin Philip +7 more
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Aneuploidy alleviates the cell proliferation defect caused by mutations affecting origin licensing in Saccharomyces cerevisiae. [PDF]
PLoS GeneticsAlthough aneuploidy is generally detrimental to the survival and growth of normal cells, it can be beneficial under certain stress conditions, such as those caused by harmful mutations.
Christophe de La Roche Saint-André
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