Results 81 to 90 of about 207,238 (265)

RNAP-II molecules participate in the anchoring of the ORC to rDNA replication origins. [PDF]

PLoS ONE, 2013
The replication of genomic DNA is limited to a single round per cell cycle. The first component, which recognises and remains bound to origins from recognition until activation and replication elongation, is the origin recognition complex.
Maria D Mayan
doaj   +1 more source

Association of the Origin Recognition Complex with Heterochromatin and HP1 in Higher Eukaryotes [PDF]

Cell, 1997
The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes.
Pak, Daniel T.S, Pflumm, Michelle, Chesnokov, Igor, Huang, Da Wei, Kellum, Rebecca, Marr, Jacqueline, Romanowski, Piotr, Botchan, Michael R   +7 more
openaire   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA [PDF]

, 2013
26.03.14 KB.
Cecile Evrin, Kawakami, H, Li, H, Riera, A, Alejandra Fernández-Cid, Jingchuan Sun, Riera, A., Stillman, B, Stillman, B., Stefan A Samel, Christian Speck, Hironori Kawakami, Speck, C., Samel, S. A., Fernandez-Cid, A., Samel, AS, Speck, C, Sun, J., Sun, J, Fernandez-Cid, A, Huilin Li, Evrin, C., Kawakami, H., Evrin, C, Li, H., Alberto Riera, Bruce Stillman   +26 more
core   +1 more source

ATP bound to the origin recognition complex is important for preRC formation [PDF]

Proceedings of the National Academy of Sciences, 2001
The origin recognition complex (ORC) binds origins of replication and directs the assembly of a higher order protein complex at these sites. ORC binds and hydrolyzes ATP in vitro . ATP binding to the largest subunit of ORC, Orc1p, stimulates specific binding to origin DNA; however, the function of ATP hydrolysis by ...
R D, Klemm, S P, Bell
openaire   +2 more sources

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Characterisation of Xenopus Origin Recognition Complex [PDF]

, 2000
The Origin Recognition Complex (ORC) of S. cerevisiae plays a crucial role in recognition of origins of DNA replication within the yeast genome. In Xenopus egg extracts DNA replication initiates from random DNA sequences suggesting that origins of DNA ...
Tugal, Tamara
core  

Cell Cycle Dependent Regulation of the Origin Recognition Complex [PDF]

Cell Cycle, 2004
The eukaryotic origin recognition complex (ORC) not only selects the sites where prereplication complexes are assembled and DNA replication begins, it is the first in a series of multiple coherent pathways that determines when prereplication complexes are assembled. Data from yeast, frogs, flies and mammals present a compelling case that one or more of
openaire   +2 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source