Purification and Characterization of theSchizosaccharomyces pombe Origin Recognition Complex [PDF]
Journal of Biological Chemistry, 2002 Ray Y. Chuang +3 more
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Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
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The origin recognition complex: from simple origins to complex functions [PDF]
Genes & Development, 2002 Eukaryotic DNA replication is an intricate process that requires the coordinate and tightly regulated action of numerous molecular machines. Eukaryotic cells must choose hundreds if not thousands of origins of replication and assemble multiple proteins or protein complexes at these sites. During S phase, these complexes are activated in a reproducible,
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International Journal of General Medicine, 2021 Shaohua Chen,1,2 Ziyao Jin,3 Linfeng Xin,4 Lv Lv,2 Xuemei Zhang,3 Yizhen Gong,5 Jianlun Liu1 1Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nangning, People’s Republic of China; 2Department of Breast and Thyroid Surgery ...
Chen S +6 more
doaj
In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization [PDF]
, 2013 The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2–7 onto DNA. Helicase loading involves two MCM2–7 hexamers that assemble into a double hexamer around
A. Fernandez-Cid +47 more
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Structural basis for origin recognition complex 1 protein-silence information regulator 1 protein interaction in epigenetic silencing [PDF]
, 2005 The interaction between silence information regulator 1 protein (Sir1p) and origin recognition complex 1 protein (Orc1p), the largest subunit of the origin recognition complex, plays an important role in the establishment of transcriptional silencing at ...
Hsu, H. C., Stillman, B., Xu, R. M.
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RNA‐dependent recruitment of the origin recognition complex [PDF]
The EMBO Journal, 2008 The origin recognition complex (ORC) has an important function in determining the initiation sites of DNA replication. In higher eukaryotes, ORC lacks sequence-specific DNA binding, and the mechanisms of ORC recruitment and origin determination are poorly understood.
Julie, Norseen +5 more
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Cdc6 ATPase activity regulates ORC center dot Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication [PDF]
, 2007 DNA replication, as with all macromolecular synthesis steps, is controlled in part at the level of initiation. Although the origin recognition complex ( ORC) binds to origins of DNA replication, it does not solely determine their location.
Speck, C., Stillman, B.
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The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]
, 2012 Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Anton Gartner +56 more
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The origin recognition complex is dispensable for endoreplication in Drosophila [PDF]
Proceedings of the National Academy of Sciences, 2008 The origin recognition complex (ORC) is an essential component of the prereplication complex (pre-RC) in mitotic cell cycles. The role of ORC as a foundation to assemble the pre-RC is conserved from yeast to human. Furthermore, in metazoans ORC plays a key role in determining the timing of replication initiation and origin usage ...
So Young Park, Maki Asano
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