Results 241 to 250 of about 5,800,592 (273)

Oxford Nanopore MinION Sequencing and Genome Assembly

open access: yesGenomics, Proteomics and Bioinformatics, 2016
Abstract The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for ...
Francesca Giordano, Zemin Ning
exaly   +4 more sources

Assessing the performance of the Oxford Nanopore Technologies MinION

open access: yesBiomolecular Detection and Quantification, 2015
The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can produce data in real-time.
Thomas W Laver, David J. Studholme
exaly   +4 more sources

Oxford Nanopore sequencing: new opportunities for plant genomics? [PDF]

open access: yesJournal of Experimental Botany, 2020
Abstract DNA sequencing was dominated by Sanger’s chain termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can
Rod J Snowdon   +2 more
exaly   +5 more sources

Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe

2020
Recent years have seen great progresses in third-generation sequencing. New commercial platforms from Oxford Nanopore Technologies (ONT) can generate ultra-long reads from single-molecule nucleic acid fragments of kilobases up to megabases, exceeding the limitation of short reads and dependency on template amplification suffered by the previous ...
Ming, He, Xu, Chi, Jie, Ren
openaire   +2 more sources

1D Genome Sequencing on the Oxford Nanopore MinION

Current Protocols in Human Genetics, 2017
AbstractToday's short‐read sequencing instruments can generate read lengths between 50 bp and 700 bp depending on the specific instrument. These high‐throughput sequencing approaches have revolutionized genomic science, allowing hundreds of thousands of full genomes to be sequenced, and have become indispensable tools for many researchers. With greater
Sara, Goodwin   +2 more
openaire   +2 more sources

A first look at the Oxford Nanopore MinION sequencer

Molecular Ecology Resources, 2014
AbstractOxford Nanopore's third‐generation single‐molecule sequencing platform promises to decrease costs for reagents and instrumentation. After a 2‐year hiatus following the initial announcement, the first devices have been released as part of an early access program. We explore the performance of this platform by resequencing the lambda phage genome,
Alexander S, Mikheyev, Mandy M Y, Tin
openaire   +2 more sources

Oxford Nanopore raises $140 million

Chemical & Engineering News, 2018
The British DNA sequencer company Oxford Nanopore Technologies has raised $140 million from a group of investors. The company sells the MinION, a popular handheld gene sequencer that can be plugged into a laptop to read DNA sequences in remote locations, including aboard the International Space Station.
exaly   +2 more sources

Approaches to Whole Mitochondrial Genome Sequencing on the Oxford Nanopore MinION

Current Protocols in Human Genetics, 2019
AbstractTraditional approaches for interrogating the mitochondrial genome often involve laborious extraction and enrichment protocols followed by Sanger sequencing. Although preparation techniques are still demanding, the advent of next‐generation or massively parallel sequencing has made it possible to routinely obtain nucleotide‐level data with ...
Roxanne R, Zascavage   +5 more
openaire   +2 more sources

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