Results 91 to 100 of about 23,440 (247)

Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

Step-by-step protocol describing the workflow for ONT direct RNA sequencing including: - RNA extraction - mRNA poly(A) selection - library preparation for ONT direct RNA sequencing - ONT direct RNA data analysis
Anita Adami, Raquel Garza
openaire   +1 more source

Dynamics of Heparin Translocations Through Solid‐State Nanopores

ELECTROPHORESIS, EarlyView.
Carbohydrates can be detected with solid‐state nanopores, but their kinetics of voltage‐driven passage are fast. To better understand the dynamics of passage of these biomolecules and the role of electrophoresis and electroosmosis, we studied the translocation characteristics of heparin as a function of salt concentration, pH and voltage polarity ...
Kexin Zhao, Matthew Waugh, Kyle Briggs, Mohammad P. Jamshidi, Janelle Sauvageau, Vincent Tabard‐Cossa   +5 more
wiley   +1 more source

A Method for Filtering Abnormal Modified Base Calling in Oxford Nanopore Technologies Sequencing

Journal of Bioinformatics and Systems Biology
Abstract Background gene synthesis sequencing using the long-read Oxford Nanopore Technologies (ONT) provides a cost-effective option for gene synthesis quality control. Despite the advantage of using long reads, however, accurate base calling is influenced by modified bases.
Ruikai Jia, Yusha Wang, Hua Ye, Xiaoshu Ma   +3 more
openaire   +1 more source

Improving genetic diagnosis of hereditary tumor syndromes: From expanded gene panels to functional genomics

International Journal of Cancer, EarlyView.
Abstract Genetic tumor risk syndromes (genturis) contribute substantially to the overall cancer burden and provide opportunities for early detection, prevention, and individualized treatment. Yet, many affected individuals remain undiagnosed due to restrictive testing criteria and challenges in variant interpretation.
Mayra Sauer, Morghan C. Lucas, Vitus Prokosch, Thomas Keßler, Thomas Risch, Andreas Laner, Jan Henkel, Anna Benet‐Pagès, Ariane Hallermayr, Verena Steinke‐Lange, Elke Holinski‐Feder, Barbara Klink   +11 more
wiley   +1 more source

Gamma SARS‐CoV‐2 variant of concern infection repercussions on pregnancy outcomes: A translational cohort study

International Journal of Gynecology &Obstetrics, EarlyView.
Abstract Objective This study conduct viral genome sequencing among severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)‐infected pregnant and postpartum individuals, and investigates disease severity and maternal and perinatal outcomes considering variant of concern (VOC) and non‐VOC groups.
Guilherme M. Nobrega, Fabiana Granja, Mariene R. Amorim, Luciana S. Mofatto, Daniel A. Toledo‐Teixeira, Julia Forato, Priscilla P. Barbosa, Jose Paulo S. Guida, Adriana G. Luz, Giuliane J. Lajos, Carolina C. Ribeiro‐do‐Valle, Gisele A. Pedroso, Kamila C. S. Krywacz, Angelica Z. Schreiber, Giuliana D. Moreno, Rodrigo N. Angerami, Maria Luiza Moretti, Rodolfo C. Pacagnella, Renato T. Souza, Jose Guilherme Cecatti, Rodrigo G. Stabeli, Magnun N. N. Santos, Fernando R. Spilki, Ester C. Sabino, Nuno R. Faria, William M. de Souza, Jose Luiz Proenca‐Modena, Maria Laura Costa   +27 more
wiley   +1 more source

The plant microbiome: From ecological foundations to precision microbial engineering for sustainable agriculture

iMeta, EarlyView.
From ecological principles to precision engineering of plant microbiomes for sustainable agriculture. The plant holobiont integrates diverse microbial niches (including rhizosphere, endosphere, phyllosphere, seed, and aerial‐root mucilagesphere), host genetics (namely M genes), and host metabolites. Furthermore, molecular dialogues, including rhizobial
Mi Wei, Tengxiang Lian, Liying Chen, Lanxiang Wang, Junjie Ye, Xiaofang Yao, Shilong Duan, Ziyu Lu, Jinji Tu, Hongfu Li, Xin‐Yue Xu, Jie Zhou, Junliang He, Feiying Zhu, Paola Bonfante, Lam‐Son Phan Tran, Zhiqiang Pang, Xin Zhou, Zhihui Xu, Lin Zhang, Mengcen Wang, Xiaolin Wang, Chang‐Fu Tian, Yong‐Xin Liu, Kai Sun, Ertao Wang, Xianan Xie   +26 more
wiley   +1 more source

Comparison of Illumina and Oxford Nanopore Technology systems for the genomic characterization of Streptococcus pneumoniae

Microbiology Spectrum
ABSTRACT Whole-genome sequencing (WGS) is an invaluable tool that enables high-resolution genotyping to precisely identify bacterial strains. It is particularly significant for highly pathogenic bacteria such as Streptococcus pneumoniae
Fatima Dakroub, Fata Akl, Alissar Zaghlout, Jose Rita Gerges, Nancy Hourani, Celina F. Boutros, George F. Araj, Ghassan M. Matar, Antoine Abou Fayad, Ghassan S. Dbaibo, Nada Ghosn, Nour Nahouli, Razane El Rifai, Zeina Mohsen, Rita Feghali, Tamima Jisr, Nour Zaatary, Emma Abboud, Marwan Osman, Elie Bou Raad, Hadi El Amin, Hicham Abdo, Rouba Chaker, Jacques E. Mokhbat, Mohamad El-Zaatari, Elie Salem Sokhn, Raymond Rohban, Elie Khoury, Nour Youssef, Danielle Fayad, Marc Finianos, Amena A. Rizk, Mireille Lteif, Bassam El Hafi, Mohammad Bahij Moumneh, Sera Chamseddine, Zeina El Houry, Magda Haj, Ahmad Chmaisse, Samer Bou Karroum, Zeinab El Zein, Yorgo Zehlani, Carine Hedari, Moza Hammadi, Zainab Ali, Imad Akel, Randa Saad, Sarah Hasan, Alice Recho, Mariam Saadeddine, Najwa Radwan, Christian Sadaka   +51 more
openaire   +3 more sources

Microbial DNA methylation links rumen transcriptional activity to methane emission divergence in dairy cows

iMeta, EarlyView.
Genome‐resolved methylomics uncovers the first DNA methylation atlas of the dairy cow rumen microbiome. Microbial DNA methylation profiles diverge between low‐ and high‐ methane‐emitting dairy cows despite limited taxonomic differences. Differential methylation is linked to transcriptional variation in genes involved in carbon metabolism, hydrogen ...
Xinyue Zhang, Guirong Xu, Liwei Zhao, Ye Tao, Jiawei Zhang, Cameron E. F. Clark, Yangyi Hao, Zhijun Cao, Shengli Li, Wei Wang   +9 more
wiley   +1 more source

Laparoscopic Methylene Blue Photodynamic Therapy for Intraabdominal Infection: A Preclinical Feasibility Study in a Rabbit Model of Perforated Appendicitis

Lasers in Surgery and Medicine, EarlyView.
ABSTRACT Objectives Perforated appendicitis commonly results in intra‐abdominal infection requiring prolonged antibiotic therapy. Intraoperative antimicrobial adjuncts are limited, and photodynamic therapy (PDT) may provide a targeted, resistance‐independent approach.
Timothy M. Baran, Korry T. Wirth, Matthew M. Byrne, Benjamin Gertsen, Eric Y. Wang, Darrian S. Hawryluk, Martin S. Pavelka, Nicole A. Wilson   +7 more
wiley   +1 more source

From Uncertainty to Pathogenicity: Resolving a CSF1R Variant of Uncertain Significance Using Long‐Read Transcriptomics

Movement Disorders, EarlyView.
Abstract Background CSF1R‐related disorder (CSF1R‐RD) is a severe autosomal dominant leukoencephalopathy characterized by progressive cognitive, neuropsychiatric, and motor decline. Although genetic testing is widely available, numerous likely pathogenic variants in CSF1R frequently remain classified as variants of uncertain significance (VUS ...
Charles Wade, Kylie Montgomery, Gabriela E. Jones, Peter Sellars, Akram A. Hosseini, Simon Paine, Henry Houlden, Mina Ryten, David S. Lynch   +8 more
wiley   +1 more source