Results 91 to 100 of about 11,306,965 (261)

Long‐Read Pan‐Cancer Transcriptomics Unravel Distinct Alteration Trends Between Gene and Isoform Expression in Tumorigenesis

iMetaMed, EarlyView.
Long‐read profiling of 144 tumor‐normal pairs identifies isoform‐level cancer dysregulation independent of gene changes. Our scoring system prioritizes isoform‐driven prognostic genes across cancers. ABSTRACT Tumorigenesis involves transcriptomic alterations at both gene and isoform levels.
Yuying Ding, Bo Wu, Dan Zhou, Qiang Li, Dingru Li, Mengyun Cai, Zhiyan Chen, Caimei Zhong, ChuanLe Xiao, Yuhua Deng   +9 more
wiley   +1 more source

Improving droplet interface platforms for nanopore sensing

Nanopore sensors have emerged since their conception 3 decades ago as a versatile, bio- inspired tool by which to conduct single-molecule investigations of DNA, and more recently proteins, RNA, methylated DNA and other polymers.
Rogers-Simmonds, Daisy
core   +1 more source

Sorghum T2T pangenome highlights comprehensive genomic variation

iMetaOmics, EarlyView.
Three newly assembled telomere‐to‐telomere (T2T) genomes representing wild, landrace, and transformable germplasm were integrated with seven publicly available T2T genomes to construct a graph‐based pangenome. Comparative analyses revealed extensive centromere divergence, including distinct CEN137 monomer and CEN274 dimer organization, and showed that ...
Lihua Wang, Chuanzheng Wei, Hope O. Okunbor, Xiaofei Deng, Die Hu, Yongfei Wang, Junli Du, Ephrem Habyarimana, Yongfu Tao, Yi‐Hong Wang, Jieqin Li   +10 more
wiley   +1 more source

Multi‐omics–driven precision medicine

iMeta, EarlyView.
Multi‐omics‐driven precision medicine (MODPM) provides a multiscale, continuously learnable framework that integrates genomics, epigenomics, transcriptomics, proteomics, metabolomics, microbiome profiles, and clinical data. Powered by artificial intelligence and foundation models, MODPM enables cross‐modal representation learning, contextual modeling ...
Huibo Li, Zhe Zhao, Yifan Zhang, Yao Ma, Gaofei Hu, Min Zeng, Zhanqun Yang, Zixuan Zhao, Xin Zhou, Wei Hu, Yuxuan Sun, Meng Su, Jun Li, Matthew Whiteman, Wei Fu, Chao Zhong, Lemin Zheng, Long Chen, Hairong Lv, Rongsheng Zhao, Yi Zhun Zhu   +20 more
wiley   +1 more source

Nanopore sequencing in veterinary medicine: from concepts to clinical applications

Frontiers in Cellular and Infection Microbiology
Oxford Nanopore Technologies (ONT) stands at the forefront of third-generation sequencing, utilizing a nanopore sequencing approach to achieve high-throughput DNA and RNA sequencing. This technology offers several key advantages, including real-time data
Maria Chaves, Amro Hashish, Amro Hashish, Iryna V. Goraichuk, Leonardo Cardia Casserta, Megan C. Mears, Eman Gadu, Eman Gadu, Abhijeet Bakre, Ellen Ruth Alexander Morris, Mostafa M. S. Shelkamy, Mostafa M. S. Shelkamy, Swathi Nadendla, Daniel R. Perez, Mohamed El-Gazzar   +14 more
doaj   +1 more source

High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell

Nature Communications, 2018
Long-read sequencing technologies facilitate efficient and high quality genome assembly. Here Michael et al. achieve a fast reference assembly for Arabidopsis thaliana KBS-Mac-74 accession using the handheld Oxford Nanopore MinION sequencer and consumer ...
Todd P. Michael, Florian Jupe, Felix Bemm, S. Timothy Motley, Justin P. Sandoval, Christa Lanz, Olivier Loudet, Detlef Weigel, Joseph R. Ecker   +8 more
doaj   +1 more source

Icarust, a real-time simulator for Oxford Nanopore adaptive sampling [PDF]

The data underlying this article will be shared on reasonable request to the corresponding author.[Motivation]: Oxford Nanopore Technologies (ONT) sequencers enable real-time generation of sequence data, which allows for concurrent analysis during a run.
Munro, Rory, Payne, Alexander, Payne, Alex, Wibowo, Satrio, Loose, Matthew   +4 more
core   +1 more source

Deciphering transcriptome complexity via long‐read sequencing

iMeta, EarlyView.
Long‐read sequencing is transforming transcriptomics from gene‐level quantification to isoform‐resolved interpretation by directly resolving full‐length transcript structures, transcription in repetitive regions, and linked molecular features. This review provides an end‐to‐end roadmap covering sequencing platforms, library preparation strategies ...
Chuwen Xu, Jia Li, Chenxi Yin, Keying Li, Tian Wang, Shiwen Gao, Yue Yu, Cheng Chang, Ye Wang, Guoqing Tong, Liang Gong, Wen Hu, Guoliang Chai, Yilai Han, Qian Qin, Suoqin Jin, Xiufen Zou, Bo Li, Tianyuan Zhang, Ana Conesa, Yunhao Wang, Dingjie Wang   +21 more
wiley   +1 more source

Oxford Nanopore Technology-Based Identification of an Acanthamoeba castellanii Endosymbiosis in Microbial Keratitis

Microorganisms
(1) Background: Microbial keratitis is a serious eye infection that carries a significant risk of vision loss. Acanthamoeba spp. are known to cause keratitis and their bacterial endosymbionts can increase virulence and/or treatment resistance and thus significantly worsen the course of the disease.
Sebastian Alexander Scharf, Lennart Friedrichs, Robert Bock, Maria Borrelli, Colin MacKenzie, Klaus Pfeffer, Birgit Henrich   +6 more
openaire   +3 more sources

Gut Enterococcus faecium derived leucic acid mediates the antiobesity effect of dietary fiber

iMeta, EarlyView.
This study identified Enterococcus faecium as a gut bacterium with anti‐obesity activity, which alleviated adipocyte hypertrophy, dyslipidemia, and hepatic steatosis by converting leucine into leucic acid (LEA) to regulate mitochondrial oxidative phosphorylation.
Baifei Hu, Qianru Gao, Junmin Hui, Mengqiong Wang, Haiyan Gao, Kai Li, Junping Zheng, Zhe Dai, Songlin Liu, Hongtao Liu   +9 more
wiley   +1 more source