Results 71 to 80 of about 11,306,965 (261)

Reproducibility and accuracy of bacterial methylome profiling using Oxford Nanopore Technologies nanopore sequencing platform

Microbial Genomics
Background. Oxford Nanopore Technologies (ONT) enables direct methylome profiling using the Dorado basecaller, but the comparative performance of ONT-only versus hybrid-assembly reference-based methylation calling, particularly regarding inter-operator variability, remains understudied.
Taylor Schababerle, Omar Hayat, Jaeone Jung, Minh Le, Isabella Polic, Hanan Wees, Micah Bhatti, Samuel Shelburne, Xiaojun Liu, Awdhesh Kalia   +9 more
openaire   +2 more sources

Resolving MiSeq-Generated Ambiguities in HLA-DPB1 Typing by Using the Oxford Nanopore Technology [PDF]

The Journal of Molecular Diagnostics, 2019
The technical limitations of current next-generation sequencing technologies, combined with an ever-increasing number of human leukocyte antigen (HLA) alleles, form the basis for the additional ambiguities encountered at an increasing rate in clinical practice.
Jamie L, Duke, Timothy L, Mosbruger, Deborah, Ferriola, Nilesh, Chitnis, Taishan, Hu, Nikolaos, Tairis, David J, Margolis, Dimitri S, Monos   +7 more
openaire   +2 more sources

A Personalized Haplotype‐Resolved Near‐Gapless Genome Framework for Somatic Variant Discovery in Hepatocellular Carcinoma

Advanced Science, EarlyView.
A patient‐specific, haplotype‐resolved genome framework improves detection and interpretation of somatic variants in hepatocellular carcinoma. Using multi‐platform sequencing, the personalized assembly resolves complex regions including centromeres and MHC/HLA loci, enhances structural variant discovery, and links regulatory alterations to allele ...
Jiazheng Lin, Yao Xiao, Shuangzi Luo, Yifan Li, Wenfei Zhang, Xiaoyu Zhang, Xinyi Wang, Di Wu, Jieru Hong, Yongkun Luan, Xun Huang, Yixiu Wang, Ningbo Wu, Xiaoxiao Hu, Fei Zhao, Wei Li, Xiao Luo, Bi Shi   +17 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer [PDF]

, 2016
Abstract Nanopore sequencing instruments measure the change in electric current caused by DNA transiting through the pore. In experimental and prototype nanopore sequencing devices it has been shown that the electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine.
Simpson, Jared T, Workman, Rachael, Zuzarte, P. C., David, Matei, Dursi, L. J., Timp, Winston   +5 more
openaire   +1 more source

Atmospheric Water Harvesting Through Polymer Hydrogel Sorbents: Materials Chemistry, Design Strategies, and Applications

Advanced Science, EarlyView.
Polymer‐based hydrogels have emerged as promising materials for atmospheric water harvesting (AWH) due to their high water affinity, tunable network structure, and excellent moisture adsorption‐desorption performance. Functional hydrogel systems enable efficient water capture and controlled release under low‐energy conditions, offering a sustainable ...
Swagata Datta, Sushmit Sen, Nilanjan Dey, Amrita Chatterjee, Ananya Sadhu, Sudipta Sarkar, Pradip K. Maji   +6 more
wiley   +1 more source

Whole genome sequencing in animal health: applications, challenges, and future directions

BMC Veterinary Research
WGS is revolutionizing animal health diagnostics, offering unprecedented opportunities for pathogen detection and biomarker identification, while offering increased depth of data, in comparison to more targeted approaches such as amplicon sequencing ...
Johannes Charlier, Alistair Antonopoulos, Bart J.G. Broeckx, Aaron Pomerantz, Veronica L Fowler, Volodimir Olexiouk, Sebastiaan Theuns, Sven Arnouts   +7 more
doaj   +1 more source

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

Methylome Profiling of a Deuterostome Invertebrate Using Oxford Nanopore Technology ( ONT )

Molecular Ecology Resources
ABSTRACT DNA methylation is crucial for genome regulation and provides key insights into the interaction between genetics and environmental factors, offering valuable perspectives for ecological research. However, knowledge of DNA methylation patterns in nonmodel invertebrates remains limited.
Sarah Lok Ting Kwong, Alyssa Maree Budd, Julia Yun‐Hsuan Hung, Cecilia Villacorta‐Rath, Sven Uthicke   +4 more
openaire   +2 more sources

Site Data from an Earthwork Survey and Archaeological Evaluation at Oxford North, Wolvercote, Oxford 2020

, 2021
This collection comprises site data (images, gis and reports) from an earthwork survey, field evaluation and test pitting on land at Wolvercote, North Oxford, undertaken by Oxford Archaeology (OA) and commissioned by EDP.
Oxford Archaeology (South)
core   +1 more source