Results 71 to 80 of about 25,431 (290)

Comparison of R9.4.1/Kit10 and R10/Kit12 Oxford Nanopore flowcells and chemistries in bacterial genome reconstruction

, 2023
Complete, accurate, cost-effective, and high-throughput reconstruction of bacterial genomes for large-scale genomic epidemiological studies is currently only possible with hybrid assembly, combining long- (typically using nanopore sequencing) and short ...
Street, teresa, Stoesser, Nicole, Crook, derrick, Rodger, gillian, Sanderson, Nicholas, Kapel, Natalia, Peto, Timothy, Lipworth, samuel, Webster, Hermione   +8 more
core   +1 more source

Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer [PDF]

, 2016
Abstract Nanopore sequencing instruments measure the change in electric current caused by DNA transiting through the pore. In experimental and prototype nanopore sequencing devices it has been shown that the electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine.
Simpson, Jared T, Workman, Rachael, Zuzarte, P. C., David, Matei, Dursi, L. J., Timp, Winston   +5 more
openaire   +1 more source

T2T Genome Assembly and Multi‐Omics Data Reveal Terrestrial Adaptation and Mucus Biosynthesis in Tropical Leatherleaf Slug (Laevicaulis alte)

Advanced Science, EarlyView.
A gap‐free genome assembly and multi‐omics comparison of the terrestrial slug Laevichaulis alte with an aquatic relative reveal that expansion of the VEGF family orchestrates mucus production, lipid metabolism, and immune defense—highlighting key molecular innovations for conquering life on land.
Gang Wang, Shaofang He, Zhongkai Wang, Aobo Pang, Xiaoli Sun, Rongchen Liu, Fang Wang, Sisi Chen, Zhijuan Bian, Dongcheng Wei, Liuhan Wu, Siyao Zhao, Qiuting Ji, Yiqi Sun, Niying Sun, Yushinta Fujaya, Boping Tang, Kianann Tan, Daizhen Zhang, Lianfu Chen   +19 more
wiley   +1 more source

Whole genome sequencing in animal health: applications, challenges, and future directions

BMC Veterinary Research
WGS is revolutionizing animal health diagnostics, offering unprecedented opportunities for pathogen detection and biomarker identification, while offering increased depth of data, in comparison to more targeted approaches such as amplicon sequencing ...
Johannes Charlier, Alistair Antonopoulos, Bart J.G. Broeckx, Aaron Pomerantz, Veronica L Fowler, Volodimir Olexiouk, Sebastiaan Theuns, Sven Arnouts   +7 more
doaj   +1 more source

Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis clade species as a test case [PDF]

PeerJ, 2018
Whole genome sequence comparisons have become essential for establishing a robust scheme in bacterial taxonomy. To generalize this genome-based taxonomy, fast, reliable, and cost-effective genome sequencing methodologies are required.
Mami Tanaka, Sayaka Mino, Yoshitoshi Ogura, Tetsuya Hayashi, Tomoo Sawabe   +4 more
doaj   +2 more sources

Resolving MiSeq-Generated Ambiguities in HLA-DPB1 Typing by Using the Oxford Nanopore Technology [PDF]

The Journal of Molecular Diagnostics, 2019
The technical limitations of current next-generation sequencing technologies, combined with an ever-increasing number of human leukocyte antigen (HLA) alleles, form the basis for the additional ambiguities encountered at an increasing rate in clinical practice.
Jamie L, Duke, Timothy L, Mosbruger, Deborah, Ferriola, Nilesh, Chitnis, Taishan, Hu, Nikolaos, Tairis, David J, Margolis, Dimitri S, Monos   +7 more
openaire   +2 more sources

Identifying N6-Methyladenosine Sites in HepG2 Cell Lines Using Oxford Nanopore Technology

International Journal of Molecular Sciences, 2023
RNA modifications, particularly N6-methyladenosine (m6A), are pivotal regulators of RNA functionality and cellular processes. We analyzed m6A modifications by employing Oxford Nanopore technology and the m6Anet algorithm, focusing on the HepG2 cell line.
Viktoriia A. Arzumanian, Ilya Y. Kurbatov, Konstantin G. Ptitsyn, Svetlana A. Khmeleva, Leonid K. Kurbatov, Sergey P. Radko, Ekaterina V. Poverennaya   +6 more
openaire   +2 more sources

Sustainable Carbon Fibers Enable Stable Long‐Term Lithium Metal Deposition for Prospective Zero‐Excess Lithium Metal Batteries

Advanced Energy Materials, EarlyView.
This work presents lightweight, lignin‐derived carbon fiber current collectors that enable controlled lithium deposition. Structural defects and intermediate‐sized pores stabilize pre‐nucleation quasi‐metallic lithium clusters, promoting uniform lithium plating and stripping.
Samantha L. S. Southern, Jonathon Cottom, Jana B. Fritzke, Emma N. Antonio, Zhenyu Guo, Bhavini Patel, Romain Tort, Ifan E. L. Stephens, Camilla Di Mino, Clare P. Grey, Rhodri Jervis, Emilia Olsson, Heather Au, Maria‐Magdalena Titirici   +13 more
wiley   +1 more source

Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies [PDF]

, 2020
Metagenomic sequence data from defined mock communities is crucial for the assessment of sequencing platform performance and downstream analyses, including assembly, binning and taxonomic assignment.
Klenk, Hans-Peter, Woyke, Tanja, Clum, Alicia, Lee, Juna, Daum, Christopher, Sevim, Volkan, Everroad, R Craig, Singer, Esther, Göker, Markus, Hundley, Hope, O’Malley, Ronan, Bebout, Brad M, Murray, Alison E, Zane, Matthew, Detweiler, Angela M, Lindemann, Stephen R, Cheng, Jan-Fang, Egan, Robert, Lee, Janey, Copeland, Alex, Pett-Ridge, Jennifer   +20 more
core   +1 more source

Methylome Profiling of a Deuterostome Invertebrate Using Oxford Nanopore Technology ( ONT )

Molecular Ecology Resources
ABSTRACT DNA methylation is crucial for genome regulation and provides key insights into the interaction between genetics and environmental factors, offering valuable perspectives for ecological research. However, knowledge of DNA methylation patterns in nonmodel invertebrates remains limited.
Sarah Lok Ting Kwong, Alyssa Maree Budd, Julia Yun‐Hsuan Hung, Cecilia Villacorta‐Rath, Sven Uthicke   +4 more
openaire   +2 more sources