Results 61 to 70 of about 11,306,965 (261)

Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires

Nature Communications
Borgs are huge extrachromosomal elements (ECE) of anaerobic methane-consuming “Candidatus Methanoperedens” archaea. Here, we used nanopore sequencing to validate published complete genomes curated from short reads and to reconstruct new genomes.
Marie C. Schoelmerich, Lynn Ly, Jacob West-Roberts, Ling-Dong Shi, Cong Shen, Nikhil S. Malvankar, Najwa Taib, Simonetta Gribaldo, Ben J. Woodcroft, Christopher W. Schadt, Basem Al-Shayeb, Xiaoguang Dai, Christopher Mozsary, Scott Hickey, Christine He, John Beaulaurier, Sissel Juul, Rohan Sachdeva, Jillian F. Banfield   +18 more
doaj   +1 more source

Combined use of Oxford Nanopore and Illumina sequencing yields insights into soybean structural variation biology

BMC Biology, 2022
Background Structural variants (SVs), including deletions, insertions, duplications, and inversions, are relatively long genomic variations implicated in a diverse range of processes from human disease to ecology and evolution.
Marc-André Lemay, Jonas A. Sibbesen, Davoud Torkamaneh, Jérémie Hamel, Roger C. Levesque, François Belzile   +5 more
doaj   +1 more source

Assessing the Use of Oxford Nanopore Technologies Sequencing for Detection of Stable Wolbachia Bacterial Strain in Symbiosis in Wild Anopheles Mosquitoes From the WHO African Region. [PDF]

Environ Microbiol
This study assesses Oxford Nanopore Technology sequencing to detect Wolbachia in Anopheles mosquitoes. To do this, samples were analysed using the ONT field sequencing kit and sequenced using a minION flow cell and the ONT 16S barcoding kit and Rapid barcoding kit sequenced using Flongle flow cells.
Chatterley L, Hull N, Hughes I, Sougoufara S, Meek O, Ceyran M, Dhokiya V, Bandibabone J, Adala C, Cunningham E, Awandu SS, Kaminski-Nenkam H, Nkondjio A, Hughes GL, Heinz E, Walker T.   +15 more
europepmc   +2 more sources

Confinement in Metal‐Organic Frameworks as a Route to Harnessing Liquid Barocalorics in the Solid‐State

Advanced Functional Materials, EarlyView.
Encapsulation of solid–liquid barocalorics (BC) within MOFs harnesses their colossal BC performance whilst allowing active control of BC properties through BC‐MOF interactions. ABSTRACT Barocaloric (BC) effects at liquid–vapor transitions in hydrofluorocarbons drive most commercial technologies used for heating and cooling in the heating, ventilation ...
Ming Zeng, Frederic Rendell‐Bhatti, Eamonn T. Connolly, Yang Wang, Clàudia Pérez‐Junyent, Josep‐Lluís Tamarit, Ross S. Forgan, Pol Lloveras, David Boldrin   +8 more
wiley   +1 more source

Consistent ultra-long DNA sequencing with automated slow pipetting

BMC Genomics, 2021
Background Oxford Nanopore Technologies’ instruments can sequence reads of great length. Long reads improve sequence assemblies by unambiguously spanning repetitive elements of the genome.
Trent M. Prall, Emma K. Neumann, Julie A. Karl, Cecilia G. Shortreed, David A. Baker, Hailey E. Bussan, Roger W. Wiseman, David H. O’Connor   +7 more
doaj   +1 more source

Iterative Selection of DNA Nanostructures for Cellular Uptake

Advanced Materials, EarlyView.
DNA nanostructures are promising cell targeting delivery vehicles for therapeutics, but the targeting behavior is not fully understood. In this study, libraries of DNA nanostructures that can be amplified and sequenced are combined with cellular uptake as a selection pressure to iteratively refine DNA structures taken up in cells to better understand ...
Anjali Rajwar, Lisa Eichhorn, Jakub Palacka, Sandra Ly, Erik Benson   +4 more
wiley   +1 more source

The first genome assembly of fungal pathogen Pyrenophora tritici-repentis race 1 isolate using Oxford Nanopore MinION sequencing

BMC Research Notes, 2021
Objectives The assembly of fungal genomes using short-reads is challenged by long repetitive and low GC regions. However, long-read sequencing technologies, such as PacBio and Oxford Nanopore, are able to overcome many problematic regions, thereby ...
Paula Moolhuijzen, Pao Theen See, Caroline S. Moffat   +2 more
doaj   +1 more source

Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies

mSystems, 2021
Advanced microbiome analysis relies on sequencing of short DNA fragments from microorganisms like bacteria, fungi, and viruses. More recently, long fragment DNA sequencing of 3rd generation sequencing has gained increasing importance and can be rapidly conducted within a few hours due to its potential real-time sequencing.
Ammer-Herrmenau, Christoph, Pfisterer, Nina, van den Berg, Tim, Gavrilova, Ivana, Amanzada, Ahmad, Singh, Shiv, Khalil, Alaa, Alili, Rohia, Belda, Eugeni, Clement, Karine, Abd El Wahed, Ahmed, Gady, Elsagad Eltayeb, Haubrock, Martin, Beißbarth, Tim, Ellenrieder, Volker, Neesse, Albrecht   +15 more
openaire   +6 more sources

Revolutionising Agricultural Sustainability: New ‘Furrow Tillage’ can Mitigate Short‐Term Soil‐to‐Atmosphere CO2 Flux and Promote Soil‐Plant‐Microbe Health

Advanced Science, EarlyView.
Furrow tillage resolves the conventional‐vs.‐no‐tillage trade‐off by simultaneously cutting CO2 efflux to 2.0–3.0 g C m−2 d−1 and unlocking high nutrient availability for the rice rhizosphere. This scalable agronomic solution strengthens soil health, enhances plant physiology, reshapes microbial metabolism, and shifts paddy systems toward a net ...
Arnab Majumdar, Munish Kumar Upadhyay, Argha Ghosh, Rakesh Biswas, Ioly Kotta Loizou, Martin Buck, Mark Tibbett, Biswajit Giri, Debojyoti Moulick, Manoj Kumar Jaiswal, Tarit Roychowdhury   +10 more
wiley   +1 more source

Atypical hepatitis B surface antigen escape mutations in an HIV-positive patient in South Africa

Southern African Journal of Infectious Diseases
A 44-year-old woman with human immunodeficiency virus (HIV), chronic kidney disease and chronic diarrhoea presented with worsening gastrointestinal symptoms and renal impairment.
Menzi B. Nkosi, Marije van Schalkwyk, Tongai G. Maponga, Yoliswa Z. Chili, Devon Muir, Paula R. Delport, Susan S. Hugo, Zahiera Ismail, Jantjie Taljaard, Gert U. van Zyl, Wolfgang Preiser   +10 more
doaj   +1 more source