Results 51 to 60 of about 23,440 (247)

The first genome assembly of fungal pathogen Pyrenophora tritici-repentis race 1 isolate using Oxford Nanopore MinION sequencing

BMC Research Notes, 2021
Objectives The assembly of fungal genomes using short-reads is challenged by long repetitive and low GC regions. However, long-read sequencing technologies, such as PacBio and Oxford Nanopore, are able to overcome many problematic regions, thereby ...
Paula Moolhuijzen, Pao Theen See, Caroline S. Moffat   +2 more
doaj   +1 more source

Identifying N6-Methyladenosine Sites in HepG2 Cell Lines Using Oxford Nanopore Technology

International Journal of Molecular Sciences, 2023
RNA modifications, particularly N6-methyladenosine (m6A), are pivotal regulators of RNA functionality and cellular processes. We analyzed m6A modifications by employing Oxford Nanopore technology and the m6Anet algorithm, focusing on the HepG2 cell line.
Viktoriia A. Arzumanian, Ilya Y. Kurbatov, Konstantin G. Ptitsyn, Svetlana A. Khmeleva, Leonid K. Kurbatov, Sergey P. Radko, Ekaterina V. Poverennaya   +6 more
openaire   +2 more sources

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

Atypical hepatitis B surface antigen escape mutations in an HIV-positive patient in South Africa

Southern African Journal of Infectious Diseases
A 44-year-old woman with human immunodeficiency virus (HIV), chronic kidney disease and chronic diarrhoea presented with worsening gastrointestinal symptoms and renal impairment.
Menzi B. Nkosi, Marije van Schalkwyk, Tongai G. Maponga, Yoliswa Z. Chili, Devon Muir, Paula R. Delport, Susan S. Hugo, Zahiera Ismail, Jantjie Taljaard, Gert U. van Zyl, Wolfgang Preiser   +10 more
doaj   +1 more source

Reproducibility and accuracy of bacterial methylome profiling using Oxford Nanopore Technologies nanopore sequencing platform

Microbial Genomics
Background. Oxford Nanopore Technologies (ONT) enables direct methylome profiling using the Dorado basecaller, but the comparative performance of ONT-only versus hybrid-assembly reference-based methylation calling, particularly regarding inter-operator variability, remains understudied.
Taylor Schababerle, Omar Hayat, Jaeone Jung, Minh Le, Isabella Polic, Hanan Wees, Micah Bhatti, Samuel Shelburne, Xiaojun Liu, Awdhesh Kalia   +9 more
openaire   +2 more sources

Resolving MiSeq-Generated Ambiguities in HLA-DPB1 Typing by Using the Oxford Nanopore Technology [PDF]

The Journal of Molecular Diagnostics, 2019
The technical limitations of current next-generation sequencing technologies, combined with an ever-increasing number of human leukocyte antigen (HLA) alleles, form the basis for the additional ambiguities encountered at an increasing rate in clinical practice.
Jamie L, Duke, Timothy L, Mosbruger, Deborah, Ferriola, Nilesh, Chitnis, Taishan, Hu, Nikolaos, Tairis, David J, Margolis, Dimitri S, Monos   +7 more
openaire   +2 more sources

An Ion‐Based Strategy Toward Synergistic Surface Functionalization Combining the Osteogenic Properties and NIR‐Mediated Antibacterial Activity of PEEK

Advanced Materials, EarlyView.
A multifunctional bioactive PEEK‐based implant (Mn@p/MBG‐SPEEK) is engineered to combat implant‐associated infections while promoting osteogenesis. The hybrid surface activates PI3K/Akt/mTOR and AP‐1 signaling pathways for osteogenic differentiation, while Mn coordination induces electronic coupling that narrows the HOMO‐LUMO energy gap and enhances ...
Zhiyan Xu, Ondřej Adam, Marek Doubrava, Ana M. Beltrán, Ivo Dlouhý, Xin Liu, Baiyan Sui, Aldo R. Boccaccini   +7 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer [PDF]

, 2016
Abstract Nanopore sequencing instruments measure the change in electric current caused by DNA transiting through the pore. In experimental and prototype nanopore sequencing devices it has been shown that the electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine.
Simpson, Jared T, Workman, Rachael, Zuzarte, P. C., David, Matei, Dursi, L. J., Timp, Winston   +5 more
openaire   +1 more source

Aggregation‐Controlled Vat Dyeing of Meta‐Aramid Fibers: Mechanistic Insights and Suppression of Surface Dye Deposition

Advanced Materials Interfaces, EarlyView.
Supramolecular aggregation governs the interfacial adsorption, diffusion, and fixation of anthraquinone‐based vat dyes in meta‐aramid fibers. By correlating reduction chemistry, aggregate formation, and fiber microstructure, a multiscale mechanism for aggregation‐controlled dyeing is established, providing an effective strategy to suppress surface dye ...
Jisiyuan Qin, Yahui Xia, Shiqi Hu, Yongmin Wu, Mohammad Raza Miah, Ruizhe Lu, Yunhan Tang, Genyang Cao, Sakil Mahmud   +8 more
wiley   +1 more source