Results 171 to 180 of about 25,431 (290)

O52: Diagnostic benefits of long-read genome sequencing for short-read negative rare disease cases

Genetics in Medicine Open
Alan Beggs, Tina Truong, Wanqing Shao, Bryan Leland, Kshitij Amar, Mehmet Bugrahan Duz, Nathaniel Edisis, Casie Genetti, Médéric Jeanne, Pratiksha Pradhan, Priyesh Rughani, Luca Schierbaum, Shirlee Shril, Rebecca Stubbs, Malika Sud, Kenney Tuyen, Carly Tyer, Talia Tzadikario, Scott Hickey, Sissel Juul, Piotr Sliz, Monica Wojcik, Friedhelm Hildebrandt, Catherine Brownstein, Miten Jain, Wendy Chung   +25 more
doaj   +1 more source

A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing

Medical and Veterinary Entomology, EarlyView.
Insectary‐reared mosquitoes were simultaneously assessed for species identification, bloodmeal source analysis and Plasmodium detection, using a single multiplexed sequencing run on the MinION sequencing platform. The method was applied to 150 insectary‐reared mosquitoes, representing nine species blood‐fed with five vertebrate hosts and 40 samples for
E. Abby Rogers, Dieunel Derilus, Christopher Sandi, Lisa Reimer, Audrey Lenhart, Lucy Mackenzie Impoinvil   +5 more
wiley   +1 more source

Fine‐tuning quantitative agronomic traits by manipulating gene copy number in rice

New Phytologist, EarlyView.
Although plant pan‐genome studies have revealed extensive copy number variations, their phenotypic consequences remain poorly understood. Here, we manipulated the copy number of OsMADS18 in rice (Oryza sativa) cv ‘Hitomebore’ using the CRISPR/Cas9 system.
Chihiro Nomura, Hiroyuki Kanzaki, Eiko Kanzaki, Motoki Shimizu, Kaori Oikawa, Hiroe Utsushi, Kazue Ito, Yusaku Sugimura, Ryohei Terauchi, Akira Abe   +9 more
wiley   +1 more source

Long‐term surveillance reveals hybridization by nuclear reassortment and intercontinental spread as major evolutionary drivers in wheat yellow rust

New Phytologist, EarlyView.
Documented incursions of Puccinia striiformis 2009–2023 based on sequential resampling of MLGs/pathogen races on different continents. Summary Evolutionary forces affecting crop pathogens, including hybridization and long‐distance dispersal (LDD), may have strong implications for food security and sustainable plant disease control at global scales ...
Mogens Støvring Hovmøller, Tine Thach, Julian Rodriguez‐Algaba, Jens Grønbech Hansen, Marcel Meyer, David P. Hodson, Kumarse Nazari, Robert F. Park, Rita Tam, Mareike Möller, Benjamin Schwessinger, John P. Rathjen, Paula Silva, Venancio Riella, Annemarie Fejer Justesen   +14 more
wiley   +1 more source

Sequencing the genomic DNA of Anodonta anatina using Oxford nanopore technology

, 2020
Freshwater mussels are members of phylum Mollusca, which live in freshwater habitats such as lakes and rivers. Freshwater mussels are essential ecologically in the aquatic ecosystems, they have a high capacity for water purification and play a significant role in calcium recycling.
openaire   +1 more source

Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding

New Phytologist, EarlyView.
Strategies for introducing genome edits into a wheat breeding program and their effects on cultivar longevity. Summary Bread wheat (Triticum aestivum) production is increasingly threatened by biotic and abiotic stresses. Developing varieties with improved stress tolerance and desirable end‐use qualities is crucial for meeting growing global demand ...
Abdulrahman Alhabsi, Francisco Miguel Ayala, Jiawei Pan, Yan L. Wang, Amira M. I. Mourad, Peter Dracatos, Brande B. H. Wulff, Yagiz Alagoz   +7 more
wiley   +1 more source

Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil. [PDF]

Sci Rep, 2023
Stevens BM, Creed TB, Reardon CL, Manter DK.   +3 more
europepmc   +1 more source

Accessory regions and horizontal gene transfer shape the evolution of clonal Colletotrichum nymphaeae infecting strawberry

New Phytologist, EarlyView.
Genetic diversity of strawberry‐infecting Colletotrichum isolates. Summary Rapid adaptation in fungal plant pathogens is often attributed to sexual recombination, yet many important pathogens are largely clonal. We investigated how genetic and phenotypic diversity arises in the predominantly asexual fungus Colletotrichum nymphaeae, the main cause of ...
Joris A. Alkemade, Alan G. Buddie, Anthony Kermode, Timothy G. Barraclough   +3 more
wiley   +1 more source

Evaluation of the accuracy of bacterial genome reconstruction with Oxford Nanopore R10.4.1 long-read-only sequencing

Whole genome reconstruction of bacterial pathogens has become an important tool for tracking transmission and antimicrobial resistance gene spread, but highly accurate and complete assemblies have largely only historically been achievable using hybrid ...
Hopkins, Katie, Stoesser, Nicole, Colpus, Matthew, Parker, Melody, Sanderson, Nicholas, Lipworth, Samuel, Crook, Derrick   +6 more
core   +1 more source

Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and Chopper. [PDF]

PLoS One
Schimke KD, Vollmers C.
europepmc   +1 more source