Results 151 to 160 of about 25,431 (290)

rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences

Yeast, EarlyView.
This work introduces a novel approach for analysing long repetitive genomic sequences, combining a method to isolate large DNA molecules from individual chromosomes. We also describe the bioinformatics tool rDNAmine, which enables the isolation of long‐repeat sequences from reads obtained using the Oxford Nanopore method, as well as their preliminary ...
Agnieszka Czarnocka‐Cieciura, Natalia Gumińska   +1 more
wiley   +1 more source

Characterization of the dynamic microbiome evolution across thrips species

Insect Science, EarlyView.
Comprehensive survey of the microbiome in thrips. The dominant bacterial genera found in thrips include intracellular ones, such as Wolbachia and Spiroplasma, and extracellular ones, including Serratia, Pantoea, and Acinetobacter. We isolated and sequenced high‐quality genomes of two dominant symbionts, Pantoea dispersa and Serratia marcescens.
Xiaodi Hu, Zhaohui Shi, Yangwei Gao, Hanze Zheng, Lin Lin, Jian‐Ping Chen, Yong Chen, Chuan‐Xi Zhang, Yiyuan Li   +8 more
wiley   +1 more source

Decoding Dual Regulatory Layers: Integrating Differential Expression and Alternative Splicing Dynamics in the Evolution of Laryngeal Echolocation Across Chiropteran Lineages

Integrative Zoology, EarlyView.
We integrated short‐read and long‐read RNA‐seq data from cochlear tissues of echolocating and non‐echolocating bats to disentangle transcriptional and post‐transcriptional regulation. Echolocating bats showed neural‐function enrichment among differentially expressed genes, while alternatively spliced genes were linked to epigenetic regulation. Overlaps
Jianyu Wu, Daoyuan Hu, Min Chen, Xiuguang Mao   +3 more
wiley   +1 more source

Feasibility of using Oxford Nanopore Technology for population genomics in Fagus sylvatica

We conducted a pilot study to evaluate the feasibility of using Oxford Nanopore Technology (ONT) to analyze genetic variation among cohorts of European beech individuals in the context of a long-term monitoring program. This is desirable due to guarantees by ONT to support reference chemistry for long-term studies and the potential to recover ...
Guerra, Toja, Coq--Etchegaray, Domitille, Li, Cheng, Schuman, Meredith C.   +3 more
openaire   +4 more sources

Addressing false negatives and positives in eDNA studies

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) has revolutionized the study of ecology and biodiversity, enabling species detection without direct observation in the environment. However, the implications of detection errors, specifically false positives and false negatives, are poorly reported in eDNA studies, significantly hindering our understanding of the ...
Amal Bajaffer, Lutfi Afiq‐Rosli, Kah Kheng Lim, Sofia Frappi, Michelle‐Nicole Havlik, Diego E. Rivera Rosas, Elisa Laiolo, Carlos Angulo‐Preckler, Carlos M. Duarte   +8 more
wiley   +1 more source

GAA‐FGF14 Ataxia Is a Frequently Overlooked Cause of Sporadic Adult‐Onset Ataxia

Clinical Genetics, EarlyView.
GAA‐FGF14 ataxia is a frequent cause of both familial and sporadic cerebellar ataxia. If symptoms are consistent, targeted testing of the FGF14 locus should be considered as a first‐line approach, as the diagnostic yield is up to 50%. ABSTRACT GAA‐FGF14 ataxia (spinocerebellar ataxia 27B, SCA27B), identified in 2023, is a major cause of adult‐onset ...
Eva‐Maria Kraus, Johannes Lenz, Pauline Ploettner, Patricia Duffek, Jost‐Julian Rumpf, Rami Abou Jamra, John Wiedenhoeft, Denny Popp   +7 more
wiley   +1 more source

Long‐read sequencing‐based atlas of tissue‐specific expression of DNM1L transcript variants

The FEBS Journal, EarlyView.
Targeted long‐read sequencing resolves full‐length DNM1L (Drp1) isoforms and reveals conserved, tissue‐specific expression patterns across human and mouse tissues. Functional assays show that Drp1 isoforms differ in their ability to drive mitochondrial fission, independent of abundance, with specific exons modulating activity.
Feng Yan, Jafar S. Jabbari, Naomi X. L. Ling, Anne M. Kong, Jia Q. Truong, Marnie L. Maddock, Ayeshah A. Rosdah, Christopher G. Langendorf, Lifang Zhang, Jarmon G. Lees, Sebastian Bass‐Stringer, Danise Ann Onda, Alice Pébay, Mirella Dottori, Kim Loh, Jessica K. Holien, Sean Lal, Nadia M. Davidson, Jonathan S. Oakhill, Shiang Y. Lim   +19 more
wiley   +1 more source

Whole Genome Assembly and Annotation of Two Invasive Sitona Species

Journal of Applied Entomology, EarlyView.
ABSTRACT Weevils are a highly diversified taxon, comprising about 70,000 described species, including many of high economic importance. Here, we present whole genome assemblies and annotations of two Sitona species (Coleoptera: Curculionidae: Entiminae).
Mandira Katuwal, Upendra Raj Bhattarai, Craig B. Phillips, Neil J. Gemmell, Eddy Dowle   +4 more
wiley   +1 more source

Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids

Journal of Fish Biology, EarlyView.
Abstract Methods using environmental nucleic acids have become highly effective for monitoring aquatic biodiversity, with an array of suitable use cases, including metrics for fisheries assessment. Traditional methods for assessing fish populations often rely on invasive techniques with limited spatial and temporal coverage.
Ana Ramón‐Laca, Krista M. Nichols, Kim M. Parsons, Ramón Gallego, David R. Vieites, Ignacio Doadrio, Ana Riesgo   +6 more
wiley   +1 more source

Double and single stranded detection of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore sequencing

Communications Biology
5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles in regulating gene expression. Using whole genomic DNA from mouse cerebellum, we benchmark 5mC and 5hmC detection by Oxford Nanopore ...
Dominic Oliver Halliwell, Floris Honig, Stefan Bagby, Sandipan Roy, Adele Murrell   +4 more
doaj   +1 more source