Results 131 to 140 of about 11,306,965 (261)

Microbiome Insights Into Zoonotic Risk at Wildlife–Human Interfaces in a Transitioning Landscape in Thailand

Integrative Zoology, EarlyView.
This study shows how ecological transitions, such as reforestation in Southeast Asia, reshape host–microbe–pathogen dynamics at the wildlife–domestic interface. By integrating long‐read microbiome sequencing, ecological gradients, and statistical and network analysis, we identify host species that disproportionately contribute to pathogen diversity and
Pauline Van Leeuwen, Chuanpot Thinphovong, Vincent Sluydts, Alix Nicolas, Anamika Kritiyakan, Areeya Kriengudom, Pipat Soisook, Kittipong Chaisiri, Serge Morand, Johan Michaux   +9 more
wiley   +1 more source

Emergence of Dengue Virus Serotype 2 Cosmopolitan Genotype, Colombia

Emerging Infectious Diseases
Using Oxford Nanopore technologies and phylogenetic analyses, we sequenced and identified the cosmopolitan genotype of dengue virus serotype 2 isolated from 2 patients in the city of Villavicencio, Meta department, Colombia. This identification suggests
David Martínez, Marcela Gómez, Carolina Hernández, Marina Muñoz, Sandra Campo-Palacio, Marina González-Robayo, Marcela Montilla, Norma Pavas-Escobar, Juan David Ramírez   +8 more
doaj   +1 more source

Oxford Nanopore Technologies: long-read sequencing for the enhancement of diagnostic analysis of rare monogenic diseases

openLa diagnosi di malattie monogeniche rare ad oggi rimane spesso incompleta a causa delle limitazioni delle tecniche di sequenziamento short-read. Pertanto, una soluzione possibile è l’utilizzo in ambito diagnostico di tecniche di sequenziamento long-
DALL'ARMI, ELENA
core  

From fluke to fragment: A multifaceted method for molecular sex identification and mitochondrial haplotyping from environmental DNA samples

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) analyses have become a powerful tool for non‐invasive biodiversity monitoring, yet the applicability of population‐genetic approaches to environmental samples remains largely unexplored. Even when genetic traces originate from a single individual, low target DNA concentrations and amplification or sequencing artefacts ...
Lauren Kelly Rodriguez, Sandra Schallhart, Philipp Hobmeier, Thomas G. Curran, Sergi Pérez‐Jorge, Rui Prieto, Cláudia Oliveira, Mónica A. Silva, Bettina Thalinger   +8 more
wiley   +1 more source

Feasibility of using Oxford Nanopore Technology for population genomics in Fagus sylvatica

We conducted a pilot study to evaluate the feasibility of using Oxford Nanopore Technology (ONT) to analyze genetic variation among cohorts of European beech (Fagus sylvatica L.) individuals in the context of a long-term monitoring program. Oxford Nanopore guarantees support of reference chemistry for long-term studies, and ONT offers potential to ...
Guerra, Toja, Coq--Etchegaray, Domitille, Li, Cheng, Schuman, Meredith C.   +3 more
openaire   +5 more sources

Enhanced fish detection sensitivity in aquatic systems using environmental RNA

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental RNA (eRNA) can potentially augment the biological monitoring ability of the established environmental DNA (eDNA) application in conservation. Despite faster degradation, environmental RNA could offer unique advantages: it may help reduce the legacy signal compared to eDNA, provide insights into physiological status through gene ...
Ana Ramón‐Laca, Ramón Gallego, Carmen Duque, José L. J. Ledesma, Ignacio Doadrio, Kim M. Parsons, Krista M. Nichols, Samantha Engster, Abigail Wells, Ana Riesgo   +9 more
wiley   +1 more source

Systematic detection of Oxford Nanopore adapter and barcode contamination in GenBank

BMC Genomics
Background Oxford Nanopore Technologies (ONT) workflows attach synthetic adapters and barcode oligonucleotides to DNA fragments for priming and multiplexing.
Florencia Martino, Abraham J. Kandathil, Steven J. Clipman   +2 more
doaj   +1 more source

EMITS: Expectation–maximization abundance estimation for fungal ITS communities from long‐read sequencing

Methods in Ecology and Evolution, EarlyView.
Abstract As long‐read amplicon sequencing becomes routine for fungal metabarcoding, species‐level abundance estimation from ITS amplicons remains limited by naive best‐hit classification. Assigning each read to its single highest‐scoring reference misattributes reads among closely related species that share similar ITS sequences, and divides the reads ...
Aaron O'Brien, Catalina Lagos, Kiara Fernández, Bárbara Ojeda, Pilar Parada   +4 more
wiley   +1 more source

Integrated Analysis of HER2 Expression and ERBB2 Alterations in Bladder Cancer

Cancer Science, EarlyView.
Integrated analysis of 445 bladder cancers showed that HER2 membrane expression and ERBB2 amplification are distinct features. HER2 expression overlapped with NECTIN4 expression, whereas ERBB2 amplification was enriched in the LumU subtype and associated with poor prognosis.
Kensuke Hirosuna, Kazuki Nishimura, Moritoshi Sakamoto, Satoshi Tokushige, Keita Nakamori, Tomohisa Matsunaga, Yuki Yoshikawa, Takeshi Tsutsumi, Ryoichi Maenosono, Takuya Tsujino, Masahiko Ajiro, Haruhito Azuma, Akihide Yoshimi, Kazumasa Komura   +13 more
wiley   +1 more source

Cross-Platform Evaluation of Established NGS-Based Metabarcoding Methods for Detecting Food Fraud in Pistachio Products

Foods
Next Generation Sequencing is a constantly evolving technology whose applicability is increasingly expanding into the field of routine food analysis.
Sina Rammouz, Jochen Riehle, Ansgar Ferner, Markus Fischer, Christian Schäfers   +4 more
doaj   +1 more source