Results 121 to 130 of about 11,306,965 (261)

Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections

Background/Objectives: The diagnosis of prosthetic joint infection remains difficult. Microbiological cultures frequently have false-positive and false-negative results.
Charlotte Hulme, Cool, Paul, Hollie Wilkinson, Hulme, Charlotte, Wright, Karina, Perry, Jade, Niall Steele, Townsend, Rob, McCarthy, Helen S., Rob Townsend, Helen S. McCarthy, Steele, Niall, Jade Perry, Wilkinson, Hollie, Benjamin Burston, Burston, Benjamin, Karina Wright, Paul Cool   +17 more
core   +1 more source

Comparative Analysis of Stress Adaptation in the Yeast Microbiome of Cactus

Yeast, EarlyView.
Yeasts and related fungi isolated from cacti or nearby non‐cactus plants were characterized phenotypically and genomically, revealing differences that hint at modes of adaptation to the cactus host. ABSTRACT Together with other fungi, yeasts make up a significant component of the plant microbiome. As the planet warms, cacti expand their range.
Alya N. Hussain, Kevin T. Fotso, Michael A. McMurray   +2 more
wiley   +1 more source

Characterization of the dynamic microbiome evolution across thrips species

Insect Science, EarlyView.
Comprehensive survey of the microbiome in thrips. The dominant bacterial genera found in thrips include intracellular ones, such as Wolbachia and Spiroplasma, and extracellular ones, including Serratia, Pantoea, and Acinetobacter. We isolated and sequenced high‐quality genomes of two dominant symbionts, Pantoea dispersa and Serratia marcescens.
Xiaodi Hu, Zhaohui Shi, Yangwei Gao, Hanze Zheng, Lin Lin, Jian‐Ping Chen, Yong Chen, Chuan‐Xi Zhang, Yiyuan Li   +8 more
wiley   +1 more source

Decoding Dual Regulatory Layers: Integrating Differential Expression and Alternative Splicing Dynamics in the Evolution of Laryngeal Echolocation Across Chiropteran Lineages

Integrative Zoology, EarlyView.
We integrated short‐read and long‐read RNA‐seq data from cochlear tissues of echolocating and non‐echolocating bats to disentangle transcriptional and post‐transcriptional regulation. Echolocating bats showed neural‐function enrichment among differentially expressed genes, while alternatively spliced genes were linked to epigenetic regulation. Overlaps
Jianyu Wu, Daoyuan Hu, Min Chen, Xiuguang Mao   +3 more
wiley   +1 more source

RNA sequencing data from the CLL cell line HG3 (HG-3) by Oxford Nanopore Technologies based direct RNA long-read sequencing.

This is the sequencing data from direct RNA long-read sequencing by Oxford Nanopore Technologies of the chronic lymphocytic leukemia cell line HG3 (HG-3). Total RNA was extracted using the RNAeasy kit (Qiagen, Hilden, Germany). Sequencing libraries were
https://orcid.org/, Richard Rosenquist Brandell   +1 more
core   +1 more source

DNA origami nanopores: developments, challenges and perspectives

, 2014
DNA nanotechnology has enabled the construction of DNA origami nanopores; synthetic nanopores that present improved capabilities for the area of single molecule detection.
Hernández-Ainsa, Silvia, Keyser, Ulrich F.   +1 more
core   +1 more source

Evolutionary and Epidemiological Characterization of Multidrug‐Resistant Escherichia coli Isolated From Pangolins in China

Integrative Zoology, EarlyView.
ABSTRACT Malayan pangolin populations have fallen to a critically endangered level, and illegal trafficking of wild pangolins is a global problem. Little is known about antimicrobial‐resistant bacteria (MDRB) harbored by pangolins, although pangolins are frequently consumed as food or used in traditional medicine. In this study, we report for the first
Yawen Wang, Xue Wang, Jianan Zhao, Jun Zeng, Shijia Hu, Fang Ji, Guoying Dong, Hongxuan He, Chengmin Wang   +8 more
wiley   +1 more source

Rapid Culture-Independent Detection of Fish Pathogens Using Oxford Nanopore Technologies: Case-Based Insights Across Multiple Species and Tissues

Pathogens
Rapid and accurate diagnosis of infectious diseases in aquaculture is essential for preventing major economic and ecological losses. Traditional culture-based methods focus on isolation of individual pathogens, and often are burdened with extended ...
Konrad Wojnarowski, Paulina Cholewińska, Dongqing Zhao, Yoshikazu Hasegawa, Daniela Denk, Dušan Palić   +5 more
doaj   +1 more source

Methodological development of human telomere sequencing with the Oxford Nanopore Technologies

, 2023
Human DNA is protected with telomeres, which consist of the repetitive DNA-sequence TTAGGG. A 3’-overhang folds back and in combination with the shelterin complex protects the ends of the DNA. Telomeres shorten over time due to the end replication problem in cell proliferation which eventually leads to senescence or apoptosis, making the telomeres an ...
openaire   +1 more source

de novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer

, 2017
International audienceBackground: Oxford Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single-molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter ...
Cruaud, Corinne, Stefan Engelen, Odette Beluche, Anne Friedrich, Payen, Emilie, Liti, Gianni, Wincker, Patrick, Lemainque, Arnaud, Caradec, Claudia, Aury, Jean-Marc, Sébastien Faye, Benjamin Istace, Engelen, Stefan, Schacherer, Joseph, Faye, Sébastien, Corinne Cruaud, Patrick Wincker, d'Agata, Léo, Claudia Caradec, Joseph Schacherer, Gianni Liti, Friedrich, Anne, Arnaud Lemainque, Beluche, Odette, Davidas, Sabrina, Léo d'Agata, Emilie Payen, Istace, Benjamin, Sabrina Davidas, Jean-Marc Aury   +29 more
core   +1 more source