Protein hydrolysates in cell culture: Toward multi‐omics characterization
Biotechnology Progress, EarlyView.While protein hydrolysates are widely used in cell culture applications, they remain undefined and variable products. Multi‐omic characterization evaluating composition and function can transition hydrolysates toward semi‐defined media components.
Michelle Combe +3 more
wiley +1 more source
Exploring papain for root canal disinfection: Antibiofilm efficacy and biocompatibility in vitro
Indian Journal of Dental ResearchIntroduction: Safer, biocompatible alternatives to NaOCl for endodontic irrigation are being explored; papain (a plant protease) could be one. Methods: E. faecalis biofilms on dentin discs treated with 4% papain, 2.5% NaOCl, or saline.
Manuel S. Thomas +2 more
doaj +1 more source
Differences between cultured cortical neurons by trypsin and papain digestion
Ibrain, 2022 The objective of this study was to compare the efficiency of trypsin and papain in neuronal digestion and determine which enzyme is more efficient. Cortical tissues were obtained from Sprague–Dawley (SD) rats. According to the different digestive enzymes,
Chang‐Yan Hu +3 more
doaj +1 more source
How to Reduce Wheat Allergens and FODMAPs in Foods: A Critical Review of Patents
Cereal Chemistry, EarlyView.ABSTRACT Background and Objectives Supported by scientific evidence, this critical review of the patent literature examined methods to reduce wheat allergens and FODMAPs in foods, which may trigger digestive disorders and other health problems. The following topics were covered: proteolytic enzymes, proteolytic bacteria and yeasts, separation, plant ...
Pierre Gélinas, Jérémie Théolier
wiley +1 more source
Detrimental Effect of Low‐Magnitude High‐Frequency Vibration in an Ex Vivo Model of Intervertebral Disc Degeneration Despite Estrogen Treatment [PDF]
JOR SpineLow‐magnitude high‐frequency vibration (LMHFV), with or without 17β‐oestradiol (E2), exacerbated papain‐induced bovine intervertebral disc degeneration, reducing cell viability, impairing matrix production, and increasing inflammation. E2 showed no protective effect against papain‐induced degeneration or LMHFV.
Jansen J +9 more
europepmc +2 more sources
Pengaruh Ph, Suhu, dan Reusability dalam Matriks Kitosan Mg Terhadap Aktivitas Immobilisasi Enzim Papain [PDF]
, 2018 Immobilization of papain enzyme is a process where by enzymes are physically placed in a certain place or space so that their catalytic activity remains and can be used repeatedly.
Gitasetiatias, Alfatkah +1 more
core
Matrix modification for enhancing the transport properties of the human cartilage endplate to improve disc nutrition. [PDF]
, 2019 Poor solute transport through the cartilage endplate (CEP) impairs disc nutrition and could be a key factor that limits the success of intradiscal biologic therapies.
Dolor, Aaron +5 more
core +2 more sources
Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning
Cytoskeleton, EarlyView.ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham +3 more
wiley +1 more source
Isolation and Characterization of Papain From The Latex of Papaya (Carica papaya L)
Indonesian Journal of Chemical Research, 2015 The papain enzyme was isolated from the latex of papaya and it was purified by ammonium sulfat fractionation and dialysis methods. Determination of papain enzyme activity showed that the highest activity was found in the 80% fraction with a specific ...
Dominggus Malle +2 more
doaj
Nanomaterials, 2020 Increasing attention has been recently paid to the development of nanocomposite materials for food application as new tool to enhance the mechanical and thermal properties of polymers.
Ilaria Benucci +3 more
doaj +1 more source