Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno‐associated Virus Delivery
Current Protocols, Volume 6, Issue 10, October 2026.Abstract This protocol describes a method for endogenous protein tagging in postmitotic primary neurons using homology‐independent targeted integration (HITI), a CRISPR/Cas9‐based approach that exploits non‐homologous end joining (NHEJ). Unlike homology‐directed repair (HDR), HITI does not require cell division, making it uniquely suited for ...
Lorena Benedetti +3 more
wiley +1 more source
IL-9 Is Essential for ILC2-Mediated but Not Th2- and Th17-Cell-Mediated Allergic Airway Inflammation in Mice. [PDF]
Eur J ImmunolSidiropoulos ND +4 more
europepmc +1 more source
Food Safety and Health, Volume 4, Issue 4, Page 908-922, October 2026.ABSTRACT Currently, small‐molecular‐weight collagen peptides are a research hotspot in functional factor development due to their high bioavailability and diverse bioactivities. In this study, gelatin was extracted from tilapia (Oreochromis niloticus) scales by hot water extraction, followed by pepsin hydrolysis and ultrafiltration to obtain the < 3 ...
Ya‐ru Liu +5 more
wiley +1 more source
Quality Improvement of Porcine Bone Broth via Enzyme-Driven Flavor Release and Structural Modification. [PDF]
J Food SciSong S +6 more
europepmc +1 more source
Extraction of Antimicrobial Peptides From Fava Bean Protein Hydrolyzate by Phosphorylated Biochar
Food Safety and Health, Volume 4, Issue 4, Page 969-983, October 2026.Antimicrobial peptides were extracted by functional biochar in one step. The enriched peptides demonstrated significantly enhanced antibacterial activity. ABSTRACT The extraction of antimicrobial peptides (AMPs) from plant proteins remains challenging due to their low abundance, structural diversity, and the need for mild, efficient, and selective ...
Xiang‐Yu Huang +8 more
wiley +1 more source
Enzymatic extraction and response surface method optimization for efficient extraction of bioactive peptides and functional activities from <i>Leucopaxillus giganteus</i>. [PDF]
Front NutrZhang C, Guo Y, Guo S, Li Y, Xu L.
europepmc +1 more source
Protocol for pH correction of FRET-based fluorescent biosensor imaging in dissociated Drosophila neurons. [PDF]
STAR ProtocPrice MS, Rastegari E, Venkatachalam K.
europepmc +1 more source
Glia, Volume 74, Issue 10, October 2026.Cell type–specific EVs were isolated from mouse brain and profiled by proteomics. Each EV subtype exhibited proteomic signatures consistent with the specialized functions of its cell of origin. Astrocyte‐derived EVs (ADEVs) were enriched for the GlialCAM/MLC1 network and GPCRs.
Alba M. Lucart‐Sanchez +7 more
wiley +1 more source
Journal of Biomedical Materials Research Part A, Volume 114, Issue 10, October 2026.ABSTRACT Articular cartilage (AC) regeneration remains a major challenge in musculoskeletal tissue engineering. Cartilage extracellular matrix (ECM)‐derived scaffolds have shown promise as tissue‐specific biomaterials, but the extent to which the fabrication process can be tuned to modulate scaffold pore architecture and mechanics and how this might ...
Giovanni Gonnella +2 more
wiley +1 more source
Comparison of the Efficiency of the Carie-Care and Bromelain Gel, as Chemomechanical Caries Removal Techniques in Primary Teeth: Randomized Controlled Clinical Trial. [PDF]
Int J Clin Pediatr DentAggarwal N +5 more
europepmc +1 more source