Results 11 to 20 of about 5,280 (145)

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets [PDF]

Nature Communications, 2018
Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to ...
Dragomir B. Krastev, Stephen J. Pettitt, James Campbell, Feifei Song, Barbara E. Tanos, Stoyno S. Stoynov, Alan Ashworth, Christopher J. Lord   +7 more
doaj   +2 more sources

Functions of PARylation in DNA Damage Repair Pathways [PDF]

Genomics, Proteomics & Bioinformatics, 2016
Protein poly ADP-ribosylation (PARylation) is a widespread post-translational modification at DNA lesions, which is catalyzed by poly(ADP-ribose) polymerases (PARPs).
Huiting Wei, Xiaochun Yu
doaj   +2 more sources

New insight into the significance of KLF4 PARylation in genome stability, carcinogenesis, and therapy

EMBO Molecular Medicine, 2020
KLF4 plays a critical role in determining cell fate responding to various stresses or oncogenic signaling. Here, we demonstrated that KLF4 is tightly regulated by poly(ADP‐ribosyl)ation (PARylation).
Zhuan Zhou, Furong Huang, Indira Shrivastava, Rui Zhu, Aiping Luo, Michael Hottiger, Ivet Bahar, Zhihua Liu, Massimo Cristofanilli, Yong Wan   +9 more
doaj   +2 more sources

TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylation [PDF]

Epigenetics & Chromatin, 2022
Background Poly(ADP-ribosyl)ation (PARylation), a posttranslational modification introduced by PARP-1 and PARP-2, has first been implicated in DNA demethylation due to its role in base excision repair. Recent evidence indicates a direct influence of PARP-
Anja Tolić, Mirunalini Ravichandran, Jovana Rajić, Marija Đorđević, Miloš Đorđević, Svetlana Dinić, Nevena Grdović, Jelena Arambašić Jovanović, Mirjana Mihailović, Nataša Nestorović, Tomasz P. Jurkowski, Aleksandra S. Uskoković, Melita S. Vidaković   +12 more
doaj   +2 more sources

Mammalian N1-adenosine PARylation is a reversible DNA modification

Nature Communications, 2022
Poly-ADP-ribosylation (PARylation) is a well-known posttranslational modification of proteins. Here the authors show that beyond proteins also mammalian single-stranded DNA is PARylated in vitro and in vivo.
Michael U. Musheev, Lars Schomacher, Amitava Basu, Dandan Han, Laura Krebs, Carola Scholz, Christof Niehrs   +6 more
doaj   +2 more sources

PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation

Nature Communications, 2021
TOP1 resolves DNA supercoils by forming cleavage complexes (TOP1cc) that are trapped by TOP1 inhibitors. Here the authors provide insights into the mechanistic understanding of TOP1cc PARylation, showing that inhibition of PARG results in stabilization ...
Yilun Sun, Jiji Chen, Shar-yin N. Huang, Yijun P. Su, Wenjie Wang, Keli Agama, Sourav Saha, Lisa M. Jenkins, John M. Pascal, Yves Pommier   +9 more
doaj   +2 more sources

PARylation of BRCA1 limits DNA break resection through BRCA2 and EXO1 [PDF]

Cell Reports, 2023
Summary: The nucleolytic processing (resection) of a DNA double-strand break (DSB) is a critical step to repair the lesion by homologous recombination (HR).
Samuele Lodovichi, Roberto Quadri, Sarah Sertic, Achille Pellicioli   +3 more
doaj   +2 more sources

Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation [PDF]

Nature Communications
The intracellular ATP-ribosyltransferases PARP1 and PARP2, contribute to DNA base excision repair (BER) and DNA demethylation and have been implicated in epigenetic programming in early mammalian development.
Simon D. Schwarz, Jianming Xu, Kapila Gunasekera, David Schürmann, Cathrine B. Vågbø, Elena Ferrari, Geir Slupphaug, Michael O. Hottiger, Primo Schär, Roland Steinacher   +9 more
doaj   +3 more sources

NAT10-mediated crosstalk between acetylation and PARylation enhances the function of PARP1 promoting primary resistance in esophageal cancer [PDF]

Cellular and Molecular Life Sciences
Resistance to platinum-based drugs represents a significant challenge in the clinical treatment of esophageal cancer. Particularly, some esophageal cancer patients exhibit primary resistance to platinum-based drugs. Our study revealed that the level of N-
Yake Chen, Sizhen Hou, Boyuan Jiang, Yang Yang, Yisi Fan, Xinyue Huang, Fengxiang Liu, Wenjing An, Qiaocen Geng, Jie Yin, Huiqin Zheng, Kexin Guo, Ting Li, Wenwen Zhao, Zhijian Deng, Di Han, Heng Li, Yu Song   +17 more
doaj   +2 more sources

ALS-FUS mutations cause abnormal PARylation and histone H1.2 interaction, leading to pathological changes

Cell Reports
Summary: The majority of severe early-onset and juvenile cases of amyotrophic lateral sclerosis (ALS) are caused by mutations in the FUS gene, resulting in rapid disease progression.
David Vílchez
exaly   +3 more sources