A comparison of the performance and application differences between manual and automated patch-clamp techniques. [PDF]
Curr Chem Genomics, 2012 The patch clamp technique is commonly used in electrophysiological experiments and offers direct insight into ion channel properties through the characterization of ion channel activity.
Yajuan X, Xin L, Zhiyuan L.
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In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives
Sensors, 2021 Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp ...
Asako Noguchi +2 more
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A novel type of nicotinic receptor in the rat central nervous system characterized by patch-clamp techniques [PDF]
Journal of Neuroscience, 1990 We present a functional characterization of a neuronal nicotinic receptor in the CNS using patch-clamp techniques and a preparation of acutely isolated neurons from the medial habenular nucleus of 10- to 20- d-old rats.
Christophe Mulle, JP Changeux
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Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience [PDF]
Journal of Venomous Animals and Toxins including Tropical DiseasesThe effect of peptide toxins on voltage-gated ion channels can be reliably assessed using electrophysiological assays, such as the patch-clamp technique.
Jessica Rojas-Palomino +5 more
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The properties of 5‐HT3 receptors in clonal cell lines studied by patch‐clamp techniques [PDF]
British Journal of Pharmacology, 1989 1 The characteristics of transmembrane currents evoked by 5‐hydroxytryptamine (5‐HT) in the neuroblastoma x Chinese hamster brain cell line NCB‐20 and neuroblastoma clonal cell line N1E‐115 have been studied under voltage‐clamp conditions by the whole ...
Jeremy J. Lambert +3 more
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Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience
Frontiers in Neural Circuits, 2015 Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal ...
Can eTao +3 more
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Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function [PDF]
Frontiers in Pharmacology, 2017 The chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the endoplasmic reticulum,
Arnaud Billet +3 more
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Patch-clamp technique to study mitochondrial membrane biophysics [PDF]
Journal of General Physiology, 2023 Mitochondria are double-membrane organelles crucial for oxidative phosphorylation, enabling efficient ATP synthesis by eukaryotic cells. Both of the membranes, the highly selective inner mitochondrial membrane (IMM) and a relatively porous outer membrane (OMM), harbor a number of integral membrane proteins that help in the transport of biological ...
Anshu Kumari, Dung M. Nguyen, Vivek Garg
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Analysis of central synaptic transmission with the slice-patch-clamp technique.
Folia Pharmacologica Japonica, 2003 More than ten years have passed since the slice-patch-clamp technique was established as a powerful method for the analysis of central synaptic transmission. Although this technique was restricted only to young animal preparations, we can now apply it to several synapses in slices obtained from adult animals, owing to recent advances in optics or ...
Toshihiko Momiyama
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Patch Clamp Techniques for Studying Ionic Channels in Excitable Membranes
Annual Review of Physiology, 1984 Availability of techniques largely influences the choice of preparations in electrophysiological research. To study basic mechanisms of electrical excita bility, usually the squid axon or giant snail neurons are used for technical reasons, or more simply, because they are large. For other reasons one would very often prefer to use mammalian cells, but
Sakmann, B., Neher, E.
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