Results 41 to 50 of about 38,690 (242)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Photocatalytic Versus Stoichiometric Hydrogen Generation Using Mesoporous Silicon Catalysts: The Complex Role of Sacrificial Reagents

Advanced Functional Materials, EarlyView.
This study highlights the importance of accounting for stoichiometric hydrogen produced when utilizing Si photocatalysts. The stoichiometric contribution is sacrificial reagent dependent and decreases with increasing sterics around the catalyst surface.
Sarrah H. Putwa, Sarah A. Martell, Berthold Reis, Simona Schwarz, Mita Dasog   +4 more
wiley   +1 more source

Defect Analysis of the β– to γ–Ga2O3 Phase Transition

Advanced Functional Materials, EarlyView.
The role of defects at all the relevant stages of the β$\beta$‐ to γ$\gamma$‐Ga2O3 polymorph transition is investigated using a multi method approach. The positron annihilation spectroscopy based results show that the defect density decreases after the transition, and that changes in defect configuration within the γ phase occur with increasing ...
Umutcan Bektas, Maciej O. Liedke, Huan Liu, Fabian Ganss, Maik Butterling, Nico Klingner, René Hübner, Ilja Makkonen, Andreas Wagner, Gregor Hlawacek   +9 more
wiley   +1 more source

The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics, 2019
This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers.
Amani Alghalayini, Alvaro Garcia, Thomas Berry, Charles G. Cranfield   +3 more
doaj   +1 more source

Harnessing Non‐Covalent Protein–Protein Interaction Domains for Production of Biocatalytic Materials Systems

Advanced Functional Materials, EarlyView.
Non‐covalent protein–protein interactions mediated by SH3, PDZ, or GBD domains enable the self‐assembly of stable and biocatalytically active hydrogel materials. These soft materials can be processed into monodisperse foams that, once dried, exhibit enhanced mechanical stability and activity and are easily integrated into microstructured flow ...
Julian S. Hertel, Maria Alessandra Martini, Marius Stoeckle, Annika J. Weber, Kersten S. Rabe, Christof M. Niemeyer   +5 more
wiley   +1 more source

Assessing Transcriptome Quality in Patch-Seq Datasets

Frontiers in Molecular Neuroscience, 2018
Patch-seq, combining patch-clamp electrophysiology with single-cell RNA-sequencing (scRNAseq), enables unprecedented access to a neuron's transcriptomic, electrophysiological, and morphological features.
Shreejoy J. Tripathy, Shreejoy J. Tripathy, Lilah Toker, Lilah Toker, Claire Bomkamp, B. Ogan Mancarci, B. Ogan Mancarci, Manuel Belmadani, Manuel Belmadani, Paul Pavlidis, Paul Pavlidis   +10 more
doaj   +1 more source

Peptide Sequencing With Single Acid Resolution Using a Sub‐Nanometer Diameter Pore

Advanced Functional Materials, EarlyView.
To sequence a single molecule of Aβ1−42–sodium dodecyl sulfate (SDS), the aggregate is forced through a sub‐nanopore 0.4 nm in diameter spanning a 4.0 nm thick membrane. The figure is a visual molecular dynamics (VMD) snapshot depicting the translocation of Aβ1−42–SDS through the pore; only the peptide, the SDS, the Na+ (yellow/green) and Cl− (cyan ...
Apurba Paul, Archith Rayabharam, Lisa Almonte, Eveline Rigo, Joshy Joseph, Ashutosh Kumar, Zhuxin Dong, Narayana Aluru, Gregory Timp   +8 more
wiley   +1 more source

Identification of a novel Gnao-mediated alternate olfactory signaling pathway in murine OSNs

Frontiers in Cellular Neuroscience, 2016
It is generally agreed that in olfactory sensory neurons (OSNs), the binding of odorant molecules to their specific olfactory receptor (OR) triggers a cAMP-dependent signaling cascade, activating cyclic-nucleotide gated (CNG) channels.
Paul eScholz, Julia eMohrhardt, Fabian eJansen, Benjamin eKalbe, Claudia eHaering, Katharina eKlasen, Hanns eHatt, Sabrina eOsterloh   +7 more
doaj   +1 more source

A Smart Bio‐Battery Facilitates Diabetic Bone Defect Repair Via Inducing Macrophage Reprogramming and Synergistically Modulating Bone Remodeling Coupling

Advanced Functional Materials, EarlyView.
This research presents a novel implantable bio‐battery, GF‐OsG, tailored for diabetic bone repair. GF‐OsG generates microcurrents in high‐glucose conditions to enhance vascularization, shift macrophages to the M2 phenotype, and regulate immune responses.
Nanning Lv, Haifu Sun, Wenxiang Tang, Yonggang Li, Zhonglai Qian, Lihui Hong, Chong Chen, Hongye Li, Jiaxiang Bai, Yusen Qiao, Mingming Liu   +10 more
wiley   +1 more source

The contribution of delta subunit-containing GABAA receptors to phasic and tonic conductance changes in cerebellum, thalamus and neocortex.

Frontiers in Neural Circuits, 2013
We have made use of the delta subunit-selective allosteric modulator DS2 (4-chloro-N-[2-(2-thienyl)imidazo[1,2-a]pyridine-3-yl benzamide) to assay the contribution of delta-GABAARs to tonic and phasic conductance changes in the cerebellum, thalamus and ...
Stephen G Brickley, Zhiwen eYe, Thomas P McGee, Catriona M Houston   +3 more
doaj   +1 more source