Results 171 to 180 of about 769,066 (216)

PCR–DGGE differentiation of strains of Saccharomyces sensu stricto

Antonie van Leeuwenhoek, 2004
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S.
M. MANZANO, COCOLIN, Luca Simone, B. LONGO, G. COMI   +3 more
openaire   +3 more sources

Use of the PCR-DGGE method in forensic microbiology

Issues of Forensic Science, 2016
The latest reports on the subject of forensic microbiology indicate that it is possible to link a perpetrator of an offence to the evidence, based on molecular analysis of microbiological material. The microbiome of human epidermis is known to be species- and individual-specific.
Aleksandra Ziembińska-Buczyńska, Krzysztof Kraśnicki   +1 more
openaire   +1 more source

Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments

Journal of Microbiological Methods, 2008
Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not
Ronald John Parkes, Andrew J. Weightman, Gordon Webster   +2 more
exaly   +2 more sources

Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE

Current Microbiology, 2009
Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens.
Akihito, Endo, Y, Futagawa-Endo, L M T, Dicks   +2 more
openaire   +2 more sources

PCR-DGGE analysis of bacterial population attached to the bovine rumen wall

Folia Microbiologica, 2010
We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken ...
Lukáš, F. (Filip), Šimůnek, J. (Jiří), Mrázek, J. (Jakub), Kopečný, J. (Jan)   +3 more
openaire   +3 more sources

Microbial diversity in lake sediments detected by PCR-DGGE

Frontiers of Biology in China, 2008
In this study, PCR-denaturing gradient gel electrophoresis (DGGE) was applied to analyze the microbial communities in lake sediments from Lake Xuanwu, Lake Mochou in Nanjing and Lake Taihu in Wuxi. Sediment samples from seven locations in three lakes were collected and their genomic DNAs were extracted.
Xinqing Zhao, Liuyan Yang, Can Chen, Lin Xiao, Lijuan Jiang, Zhe Ma, Haowei Zhu, Zhenyang Yu, Daqiang Yin   +8 more
openaire   +1 more source

Application of PCR-DGGE for Identification Microflora of Kulen

2002
The microbiology of fermented sausages is varied and complex. The type of microflora that develops is often closely related to the rippening technique utilized. Manufacturing of fermented sausages has a long tradition in croatian region Slavonija. Kulen sausage is an indigenous croatian product.
Orlić, Sandi, Redžepović, Sulejman
openaire   +2 more sources

Microbiological characterisation of Robiola di Roccaverano cheese using PCR–DGGE

Food Microbiology, 2008
The aim of this study was the microbiological characterisation of a typical Italian cheese "Robiola di Roccaverano" with Protected Designation of Origin (PDO). Fresh and ripened robiola samples were collected from four artisanal and one industrial producer.
BONETTA, SILVIA, BONETTA, Sara, CARRARO, Elisabetta, RANTSIOU, KALLIOPI, COCOLIN, Luca Simone   +4 more
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Diversity of bacterial population of table olives assessed by PCR-DGGE analysis

Food Microbiology, 2012
Nocellara Etnea and Geracese table olives are produced according to traditional process, in which lactic acid bacteria (LAB) and yeasts are the dominant microorganisms. With the aim to evaluate the effect of selected starter cultures on dynamics of bacterial population during fermentation and on growth/survival of Listeria spp.
RANDAZZO, CINZIA LUCIA, Ribbera A, Pitino I, Romeo FV, CAGGIA, Cinzia   +4 more
openaire   +3 more sources

การวิเคราะห์ความหลากหลายของประชากรจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบีโดยใช้เทคนิค PCR-DGGE

ศึกษาผลของภาระบรรทุกสารอินทรีย์และความเร็วไหลขึ้นควบคู่ไปกับ การศึกษาความหลากหลายของกลุ่มจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบี โดยแบ่งการทดลองออกเป็น 3 ช่วงคือ ช่วงที่ 1 ศึกษาประสิทธิภาพของระบบอีจีเอสบีที่ภาระบรรทุกสารอินทรีย์ต่างกัน คือ 10, 20, 30 และ 40 กก.ซีโอดี/ลบ.ม.-วัน โดยใช้ความเร็วไหลขึ้น 3 และ 4 ม./ชม.
openaire   +1 more source