Results 181 to 190 of about 769,066 (216)

Cider Apple native microbiota characterization by PCR-DGGE

2014
The surface microbiota of different recognized apple varieties used to elaborate a Protected Designation of Origin cider was studied to analyse the microbial diversity and its potential link to the microorganisms involved in the cider fermentation process.
Alonso, S., Laca, A., Rendueles, Manuel, Mayo Pérez, Baltasar, Díaz Fernández, José Mario   +4 more
openaire   +2 more sources

Analysis of the large bowel microbiota of colitic mice using PCR/DGGE

Letters in Applied Microbiology, 2005
To test combined polymerase chain reaction amplification of 16S rRNA gene sequences and denaturing gradient gel electrophoresis (PCR/DGGE) as an analytical method to investigate the composition of the large bowel microbiota of mice during the development of colitis.The colonic microbiota of formerly germfree interleukin 10 (IL-10)-deficient mice that ...
R, Bibiloni, M A, Simon, C, Albright, B, Sartor, G W, Tannock   +4 more
openaire   +2 more sources

Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE

Journal of Environmental Sciences, 2007
This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain, and found two dominant strains in ...
Qing, Hu, Min-Na, Dou, Hong-Yan, Qi, Xiang-Ming, Xie, Guo-Qiang, Zhuang, Min, Yang   +5 more
openaire   +2 more sources

Evaluation of PCR-DGGE methodology to monitor fungal communities on grapes.

Journal of applied microbiology, 2009
Some fungi present on the surface of grapes may have a negative effect on the quality of wine. The aim of this study was to evaluate PCR-denaturing gradient gel electrophoresis (PCR-DGGE), for the establishment of fungal community profiles from grapes, in order to monitor fungi potentially involved in wine defects.A fragment of the beta-tubulin gene ...
Laforgue, Rémi, Guerin, L., Pernelle, J.J., Monnet, C., Dupont, J., Bouix, Marielle   +5 more
openaire   +4 more sources

PCR-DGGE analysis of aspirates from chronic rhinosinusitis patients

International Congress Series, 2006
Abstract Middle meatus aspirates from patients with chronic rhinosinusitis were analysed by bacterial culture and non-culture techniques. PCR-denaturing gradient gel electrophoresis detected a greater bacterial diversity than culture methods. There was evidence of substantial changes in the predominant bacteria over time and two species of non ...
Dan Power, Jeremy Burton, Chris Chilcott, Patrick Dawes, John Tagg   +4 more
openaire   +1 more source

Side effects of antibiotics on the intestinal microflora by PCR-DGGE.

Pakistan journal of pharmaceutical sciences, 2013
Antibiotics are recommended for use in the treatment of infectious diseases, but the side effects have not been thoroughly investigated, especially on the intestinal tract. Fluoroquinolones, macrolides and β-lactams were tested the side effects on the intestinal microbflora of mice in this study.
Li, Xinli, Wu, Dachang, Zhang, Cuili, Xin, Yi   +3 more
openaire   +1 more source

[Application of PCR-dGGE in selective activation of stratal bacteria].

Huan jing ke xue= Huanjing kexue, 2010
With the development of molecular biology, the research on microflora in the environment is accelerated and a new research method for oil reservoir microflora is provided. Based on the methods of PCR-DGGE, the change of the structure of dominant microbial population in selectiveactivation of stratal bacteria was studied.
Mu-Tai, Bao, Bing, Wang, Qing-Guo, Chen, Guang-Jun, Gao, Xi-Ming, Li   +4 more
openaire   +1 more source

PCR DGGE and RT-PCR DGGE show diversity and short-term temporal stability in the Clostridium coccoides-Eubacterium rectale group in the human intestinal microbiota.

FEMS microbiology ecology, 2006
As the Clostridium coccoides-Eubacterium rectale (Erec; clostridial phylogenetic cluster XIVa) group is one of the major groups of the human intestinal microbiota, DNA- and RNA-based population analysis techniques (denaturing gradient gel electrophoresis; DGGE) were developed and applied to assess the diversity and temporal stability (6 months-2 years)
Maukonen, Johanna, Mättö, Jaana, Satokari, Reetta, Söderlund, Hans, Mattila-Sandholm, Tiina, Saarela, Maria   +5 more
openaire   +2 more sources

PCR-DGGE DIFFERENTIATION OF SACCHAROMYCES SENSU STRICTO STRAINS

2004
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus/S.
MANZANO, Marisa, Luca, Cocolin, Benedetta, Longo, COMI, Giuseppe   +3 more
openaire   +1 more source

Polymorphism at the porcine LEPR gene detected by PCR‐DGGE

Animal Genetics, 1997
M, Kopecný, A, Stratil, S, Cepica
openaire   +2 more sources