Rapid detection of MDR–Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens [PDF]
Asian Pacific Journal of Tropical Biomedicine, 2014 Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant based on polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique that analyzes the katG, rpoB genes.
Imani Fooladi Abbas Ali +3 more
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Molecular Characteristics of Phytophthora katsurae Using PCR-SSCP Analysis
Research in Plant Disease, 2011 Phytophthora katsurae is the fungus responsible for chestnut ink disease. The objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis of rDNA-ITS region, elongation factor 1 alpha gene and β-tubulin gene ...
Sun Keun Lee +5 more
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3′ Overhangs Influence PCR-SSCP Patterns [PDF]
BioTechniques, 2000 Rachel Forrest +2 more
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Improved Resolution of Asymmetric-PCR SSCP Products [PDF]
BioTechniques, 1997 Richard M. Pokorny +3 more
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High-Throughput Asymmetric-PCR SSCP Analysis Using Well-Controlled Temperature Conditions
BioTechniques, 1996 Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled.
Masaharu Kiyama, Takeshi Fujita
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Single nucleotide polymorphism rs7908486 of the TCF7L2 gene is highly associated with obesity in the Iraqi population [PDF]
Archives of Biological Sciences, 2021 This study was conducted to assess the possible association between polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA was obtained from 158 obese subjects and 142 controls.
Mohammed Amera K. +2 more
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Genotyping of Salmonella enterica strains from animal and human origin using three molecular techniques [PDF]
Iraqi Journal of Veterinary Sciences, 2022 This study aims to characterize different Salmonella enterica subsp molecularly. enterica strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry from Santander and Tolima regions using PCR-RFLP, PCR-ribotyping ...
Juan S. Cruz-Méndez +2 more
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Detection and isolation of nuclear haplotypes by PCR‐SSCP [PDF]
Molecular Ecology, 1997 SSCP (single‐strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated
Ortí, G, Hare, MP, Avise, JC
openaire +4 more sources
Single-Strand Conformation Polymorphism Fingerprint Method for Dictyostelids
Frontiers in Microbiology, 2021 Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not
Phongthana Pasookhush +10 more
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Molecular typing of Brucella species by PCR-RFLP and SSCP
Indian Journal of Animal Sciences, 2016 PCR-RFLP and PCR-SSCP help to understand variabilities in Brucella spp. genome which in turn assist in planning epidemiological strategies for control of brucellosis in animal population and thereby human transmission.
M V HEMADE, R S GANDGE
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