Results 21 to 30 of about 6,961 (161)

PCR-SSCP analysis of cancer related genes.

open access: yesSEIBUTSU BUTSURI KAGAKU, 2001
Single-strand DNA conformation polymorphism (SSCP) analysis has been used to detect alterations in relatively short DNA fragments such as genetic polymorphisms, mutations, deletions, and so on. Here we introduce other applications of SSCP analysis, using its capacity of separating alleles.
Masato Maekawa, Kokichi Sugano
openaire   +2 more sources

Paternal imprinting of the SLC22A1LS gene located in the human chromosome segment 11p15.5

open access: yesBMC Genetics, 2004
Background Genomic imprinting is an epigenetic chromosomal modification in the gametes or zygotes that results in a non-random monoallelic expression of specific autosomal genes depending upon their parent of origin.
Krishna Lingegowda   +3 more
doaj   +1 more source

SNP analysis of the inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene by a fluorescence-adapted SSCP method

open access: yesBMC Medical Genetics, 2002
Background Single-nucleotide polymorphisms (SNPs) are considered to be useful polymorphic markers for genetic studies of polygenic traits. Single-stranded conformational polymorphism (SSCP) analysis has been widely applied to detect SNPs, including point
Taniyama Matsuo   +4 more
doaj   +1 more source

Variant evidence of three genes of Potato vein yellow virus infecting Solanum tubersosum sp. using single strand conformational polymorphism.

open access: yesActa Biológica Colombiana, 2015
Potato yellow vein virus (PYVV) with tripartite RNA (ss+) genome is classified as Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield effect.
Karen Andrea CUBILLOS ABELLO   +1 more
doaj   +1 more source

PCR–SSCP: A Method for the Molecular Analysis of Genetic Diseases [PDF]

open access: yesMolecular Biotechnology, 2007
Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions ...
V Konstantinos, Kakavas   +9 more
openaire   +2 more sources

Evaluation of p53 Gene Mutations in Oral Lichen Planus Lesions in a Population From Iran

open access: yesClinical and Experimental Dental Research, Volume 11, Issue 5, October 2025.
ABSTRACT Objectives Oral lichen planus (OLP) is a chronic inflammatory disorder of the oral mucosa, genetic and molecular alterations, including mutations in the p53 tumor suppressor gene, have been implicated in OLP pathogenesis. However, its molecular mechanisms are not clearly understood.
Erfan Jokar   +6 more
wiley   +1 more source

DNA State Influences the Uptake and Persistence of Environmental DNA by Marine Sponge Natural Samplers

open access: yesEcology and Evolution, Volume 15, Issue 5, May 2025.
We present results from a mesocosm experiment testing the utility of marine sponges as natural samplers of eDNA and show the effect of DNA state on the capture and persistence of genetic material. We find a significant interaction between DNA state and sample type, with lower detection and concentration of extracellular DNA, compared to cellular DNA ...
Rosalie Dowell   +4 more
wiley   +1 more source

Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers

open access: yesCancer Medicine, Volume 14, Issue 8, April 2025.
ABSTRACT Background Genetic testing and sequencing technologies offer a comprehensive understanding of cancer genetics, providing rapid and cost‐effective solutions. In particular, these advanced technologies play an important role in assessing the complexities of the rare cancer types affecting several systems including the bone, endocrine, digestive,
Joviana Farhat   +4 more
wiley   +1 more source

Application of ubiquitin SSCP analysis in taxonomic studies within the subgenus Orinocarabus (Coleoptera: Carabidae: Carabus)

open access: yesEuropean Journal of Entomology, 2000
SSCP (single-strand conformation polymorphism) analyses of ubiquitin genes were used to investigate evolutionary relationships within the subgenus Orinocarabus of the genus Carabus. After SSCP electrophoresis of PCR-amplified ubiquitin copies, population-
Dieter SEDLMAIR   +2 more
doaj   +1 more source

Correlation of SNPs in Myeloid differentiation-2 (MD-2) gene with the susceptibility to clinical mastitis in Chinese Holstein dairy cows

open access: yesIndian Journal of Animal Sciences, 2021
Myeloid differentiation-2 (MD-2), as an essential component of the CD14-TLR4/MD-2 receptor complex, is critical in identifying bacterial Lipopolysaccharide (LPS) and activating innate immune responses.
JINGCHENG XU   +3 more
doaj   +1 more source

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