Results 21 to 30 of about 6,961 (161)
PCR-SSCP analysis of cancer related genes.
Single-strand DNA conformation polymorphism (SSCP) analysis has been used to detect alterations in relatively short DNA fragments such as genetic polymorphisms, mutations, deletions, and so on. Here we introduce other applications of SSCP analysis, using its capacity of separating alleles.
Masato Maekawa, Kokichi Sugano
openaire +2 more sources
Paternal imprinting of the
Background Genomic imprinting is an epigenetic chromosomal modification in the gametes or zygotes that results in a non-random monoallelic expression of specific autosomal genes depending upon their parent of origin.
Krishna Lingegowda +3 more
doaj +1 more source
Background Single-nucleotide polymorphisms (SNPs) are considered to be useful polymorphic markers for genetic studies of polygenic traits. Single-stranded conformational polymorphism (SSCP) analysis has been widely applied to detect SNPs, including point
Taniyama Matsuo +4 more
doaj +1 more source
Potato yellow vein virus (PYVV) with tripartite RNA (ss+) genome is classified as Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield effect.
Karen Andrea CUBILLOS ABELLO +1 more
doaj +1 more source
PCR–SSCP: A Method for the Molecular Analysis of Genetic Diseases [PDF]
Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions ...
V Konstantinos, Kakavas +9 more
openaire +2 more sources
Evaluation of p53 Gene Mutations in Oral Lichen Planus Lesions in a Population From Iran
ABSTRACT Objectives Oral lichen planus (OLP) is a chronic inflammatory disorder of the oral mucosa, genetic and molecular alterations, including mutations in the p53 tumor suppressor gene, have been implicated in OLP pathogenesis. However, its molecular mechanisms are not clearly understood.
Erfan Jokar +6 more
wiley +1 more source
We present results from a mesocosm experiment testing the utility of marine sponges as natural samplers of eDNA and show the effect of DNA state on the capture and persistence of genetic material. We find a significant interaction between DNA state and sample type, with lower detection and concentration of extracellular DNA, compared to cellular DNA ...
Rosalie Dowell +4 more
wiley +1 more source
ABSTRACT Background Genetic testing and sequencing technologies offer a comprehensive understanding of cancer genetics, providing rapid and cost‐effective solutions. In particular, these advanced technologies play an important role in assessing the complexities of the rare cancer types affecting several systems including the bone, endocrine, digestive,
Joviana Farhat +4 more
wiley +1 more source
SSCP (single-strand conformation polymorphism) analyses of ubiquitin genes were used to investigate evolutionary relationships within the subgenus Orinocarabus of the genus Carabus. After SSCP electrophoresis of PCR-amplified ubiquitin copies, population-
Dieter SEDLMAIR +2 more
doaj +1 more source
Myeloid differentiation-2 (MD-2), as an essential component of the CD14-TLR4/MD-2 receptor complex, is critical in identifying bacterial Lipopolysaccharide (LPS) and activating innate immune responses.
JINGCHENG XU +3 more
doaj +1 more source

