Results 21 to 30 of about 11,551 (206)
Memorias do Instituto Oswaldo Cruz, 2006 Mutations in the rpoB locus confer conformational changes leading to defective binding of rifampin (RIF) to rpoB and consequently resistance in Mycobacterium tuberculosis.
Bahram Nasr Isfahani +3 more
doaj +1 more source
Emerging Infectious Diseases, 2001 The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results.
Miriam Bobadilla-del-Valle +8 more
doaj +1 more source
Polymorphisms in swine candidate genes for meat quality detected by PCR-SSCP
Italian Journal of Animal Science, 2010 In order to identify SNPs in seven candidate genes for meat quality, PCR-SSCP experiments were carried out. Statistical analyses in a population of 240 individuals identified the animals constituting the extreme tails of the Gaussian distribution for ...
Stefania Chessa +4 more
doaj +1 more source
Identification of Myostatin Gene C.960delg Locus Polymorphism in Indonesian Local Sheep by Using Pcr-sscp Method [PDF]
, 2011 Myostatin (MSTN) is a protein inhibit muscle growth. This protein is a member of a superfamily ofmolecules called transforming growth factors beta (TGF-b).
Andreas, E. (E) +4 more
core +1 more source
PCR Mutagenesis-Based Method for Generation of Positive Controls for SSCP Analysis
BioTechniques, 1997 We have developed a primer-mediated PCR mutagenesis-based method for the generation of positive controls to test the sensitivity of single-strand conformation polymorphism (SSCP) or any other PCRbased mutation screening method. This technique is based on
A. Ruiz +4 more
doaj +1 more source
PCR-SSCP analysis of cancer related genes.
SEIBUTSU BUTSURI KAGAKU, 2001 Single-strand DNA conformation polymorphism (SSCP) analysis has been used to detect alterations in relatively short DNA fragments such as genetic polymorphisms, mutations, deletions, and so on. Here we introduce other applications of SSCP analysis, using its capacity of separating alleles.
Masato Maekawa, Kokichi Sugano
openaire +2 more sources
BMC Medical Genetics, 2002 Background Single-nucleotide polymorphisms (SNPs) are considered to be useful polymorphic markers for genetic studies of polygenic traits. Single-stranded conformational polymorphism (SSCP) analysis has been widely applied to detect SNPs, including point
Taniyama Matsuo +4 more
doaj +1 more source
Acta Biológica Colombiana, 2015 Potato yellow vein virus (PYVV) with tripartite RNA (ss+) genome is classified as Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield effect.
Karen Andrea CUBILLOS ABELLO +1 more
doaj +1 more source
Common promoter variant in cyclooxygenase-2 represses gene expression: evidence of role in acute-phase inflammatory response [PDF]
, 2002 Objective: Cyclooxygenase (COX)-2 is a key regulatory enzyme in the synthesis of prostanoids associated with trauma and inflammation. We investigated the COX-2 gene for functional variants that may influence susceptibility to disease.
Brull, D.J. +6 more
core +1 more source
PCR–SSCP: A Method for the Molecular Analysis of Genetic Diseases [PDF]
Molecular Biotechnology, 2007 Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions ...
V Konstantinos, Kakavas +9 more
openaire +2 more sources